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Articles by H.R. Kavousi
Total Records ( 2 ) for H.R. Kavousi
  H.R. Kavousi , M. Farsi and F. Shahriari
  The secondarily homothallic life cycle of the white button mushroom that results in scarcity of uninucleate basidiospores (homokaryons) in its progeny, is the most important impediment for genetic improvement of the commercial strains. Identification of homokaryons for breeding programs of Agaricus bisporus (button mushroom) is, therefore, crucial. Verifying homokaryons through fruiting trial is time consuming and unreliable. In this study, ability of RAPD markers, compared to morphological characters for identification of homokaryon isolates, was investigated. Based on morphological characters, 42 isolates were screened and exposed to RAPD markers. The results showed that RAPD markers could discriminate homokaryons from heterokaryons, based on number of bands generated. The numbers of band in homokaryons were significantly less than those of heterokaryons. Results also showed that cluster analysis, based on average of band number generated, could separate homokaryon from heterokaryon isolates. It is suggested that RAPDs could be used to identify hyomokaryons from heterokaryons for breeding program of A. bisporus.
  H.R. Kavousi , H. Marashi , J. Mozafari and A.R. Bagheri
  The fungal disease, ascochyta blight, caused by Ascochyta rabiei is a major yield limiting factor of chickpea (Cicer arietinum L.) around the world. Expression analysis of genes induced in general defense response can provide clues to elucidate major defense mechanisms against pathogen infection in chickpea plants. The role of key phenylpropanoid pathway enzymes response to Ascochyta rabiei in chickpea was studied under greenhouse conditions using a reverse transcription and semi-quantitative polymerase chain reaction (SQ-PCR). Transcript accumulation of four genes encoding phenylalanine ammonia-lyase (PAL), chalcon synthase (CHS), isoflavone reductase (IFR) and Flavanone 3-Hydroxylase (F3H) induced in response to race 3 of A. rabiei was compared in resistant and susceptible genotypes. Results obtained in this study showed that in resistant genotype all 4 phenylpropanoid pathway genes: PAL, CHS, IFR and F3H were rapidly up regulated 6 h after inoculation with race 3 of A. rabiei. However, transcripts of PAL and IFR genes were rapidly accumulated in both resistant and susceptible cultivars. Therefore, induction of key enzymes of phenylpropanoid pathway appeared to be an important defense mechanism of chickpea plants against A. rabiei.
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