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Articles by H.R. Etebarian
Total Records ( 2 ) for H.R. Etebarian
  A. Mikani , H.R. Etebarian , H. Aminian and A. Alizadeh
  Five isolates KW, 16P, 8, 11 and 7 of Pseudomonas fluorescens and fungicide thiabendazole were evaluated for control of gray mold of apple caused by Botrytis mali. Dual culture, cell free metabolite and volatile test showed that all five isolates of Pseudomonas fluorescens tested inhibited growth of the pathogen. Inhibition varied from 28.9-85.7% in dual culture, from 62-94.6% in cell free metabolite and from 45.9-87.9% in volatile test. Apples were wounded and inoculated with a 20 μL Botrytis mali (105 conidia mL-1) combined with a range of concentration of fungicide. Thiabendazole controlled gray mold by over 83% at 5 μg mL-1. Pseudomonas fluorescens isolates prevented B. mali decay from expanding to more than 49.4% at 20°C. Thiabendazole was combined with bacteria to control gray mold of apple. Mixing of thiabendazole and bacterial isolates provide more than 90.2% control at concentrations 5 μL mL-1 and 107 CFU mL-1, respectively, on Golden delicious apples. A combination of thiabendazole and Pseudomonas fluorescens isolate 11 at concentration 10 μg mL-1 and 1x107 CFU mL-1, respectively controlled gray mold by 100%. Thiabendazole was not bactericidal and had no effect on five isloates of Pseudomonas fluorescens.
  H.R. Etebarian
  Biological control of causal pathogen (Macrophomina phaseolina) was investigated by four strains of Streptomyces. Dual culture and cellophane overlay technique were used in vitro assay. All antagonist - host combination were carried out in 4 replicates. Colony area was recorded daily, compared with controls and percentage of growth inhibition was calculated. Glasshouse studies were performed to test the ability of Streptomyces strains incorporated to the soil as a rate of 10g kg-1 potting mix. In other experiment seeds of melon were soaked in Streptomyces suspension and planted in infected soil with pathogen. Streptomyces strains significantly inhibited mycelial growth of Macrophomina phaseolina in dual culture. Streptomyces strains, STL, A20, and A15 inhibited the growth of pathogen by 88.16 to 89.3% and mycelial growth of M. phseolina was reduced 69.99% by Streptomyces strain A22. Cell free metabolites produced by 4 strains of Streptomyces reduced colony area by 80.14-99.66%. The results of glasshouse experiment indicated that when soil of pots inoculated with pathogen + Streptomyces or Streptomyces alone, percentage of healthy plants were significantly greater than in those of pathogen control (p<0.05). Similar results were obtained in seed treatment test.
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