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Articles by H.P. Ju
Total Records ( 2 ) for H.P. Ju
  H.H. Musa , G.H. Chen , K.H. Wang , B.C. Li , D.M. Mekki , J.T. Shu and H.P. Ju
  Serum cholesterol, lipoprotein concentration and carcass characteristics data were taken in 30 fat and 40 lean chickens. Breeds were differed significantly (p<0.001) in carcass traits and non significantly (p>0.05) in carcass portions. Color density and pH were also differed significantly (p<0.05), whereas water holding capacity (WHC) and shear force value were non significant (p>0.05). Both breeds showed positive correlation for carcass traits, while abdominal fat was negatively related with breast muscle in lean breed. Significant difference (p<0.05) were observed in cholesterol and LDL and non significant (p>0.05) in triglyceride, VLDL and HDL level. Cholesterol and triglyceride were respectively related with LDL and VLDL. HDL was observed negatively related with triglyceride and cholesterol. Sex was effect significantly (p<0.05) in triglycerides and VLDL level and non significantly (p>0.05) in cholesterol, HDL and LDL level in fat breed. However, lean breed observed non significant difference (p>0.05) for lipoprotein concentrations. Correlation of lipoprotein concentrations and carcass trait were determined. In both breeds lipoprotein concentrations were negatively related with carcass traits. LDL was positively related with breast muscle and abdominal fat weight and triglyceride with live weight in fat breed. In lean breed cholesterol and LDL were presented positive correlation with all carcass traits.
  H.H. Musa , J.H. Cheng , X.S. Wu , H.P. Ju , D.M. Mekki and G.H. Chen
  Present study was focus to compare LDL receptor mRNA expression, total cholesterol, triglyceride, lipoprotien levels and abdominal fat weight in genetically fat and lean chickens. Genetically lean (Rugao) and fat (Anka) chickens were reared in the same environmental condition, at 12 weeks of age samples of liver tissue were collected and abdominal fat weight was determined. Similarly, total cholesterol, triglycerides and high density lipoprotein were assayed using a commercial enzymatic kit, very low density lipoprotein and low density lipoprotein were estimated using the Friedewald equation. Total RNA from liver tissues were isolated using the standard Trizol methods and then total RNA was reverse transcribed by moloney murine Leukemia virus. Semi-quantitative RT-PCR was developed to quantify the levels of LDL receptor mRNA expression. The level of LDL receptor expression was significantly (p< 0.05) difference between lean and fat chicken. In addition, lean and fat chickens were significantly differed on triglyceride, very low density lipoprotein and abdominal fat weight. The expression of LDL receptor mRNA in liver of fat chicken was negatively correlated with abdominal fat weight. However, in lean chicken was negatively correlated with total cholesterol, triglyceride, lipoprotein concentration, abdominal fat weight and percentage of abdominal fat weight. In addition, within two breeds LDL receptor mRNA expression in liver was negatively correlated with low density lipoprotein, abdominal fat weight and percentage of abdominal fat weight.
 
 
 
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