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Articles by H.M.A. Hassan
Total Records ( 11 ) for H.M.A. Hassan
  H.M.A. Hassan , M.M. El-Moniary , Y. Hamouda , Eman F. El-Daly , Amani W. Youssef and Nafisa A. Abd El-Azeem
  A growth experiment was conducted to study productive performance, carcass characteristics and some blood parameters of broiler chicks fed corn-soybean meal diets with 3 levels of Moringa oleifera leaves meal (MOLM) under heat stress conditions. Two hundred and eighty one day old chicks were randomly assigned to four treatments. The 1st treatment fed a commercial basal diet as a control, while, the other treatments 2, 3 and 4 were fed the commercial basal diet supplemented with MOLM (0.1, 0.2 and 0.3%, respectively). The results showed that body weight gain was increased significantly (p<0.05) as the level of MOLM increase; also, the feed intake had the same trend. Feed conversion ratio was recorded better values as the level of MOLM increase. The levels of MOLM had no significant effect on carcass relative weight, liver, gizzard, heart, abdominal fat, breast and thigh. Haemoglobin (Hb) was increased with increase the level of MOLM, while, haematocrit (Ht) values did not affect. Heterophil/Lymphocyte (H/L) ratio was decreased by increase the level of MOLM. Plasma total protein increased significantly (p<0.05) with increase the level of MOLM. Albumin did not affect while, globulin increased significantly (p<0.05) with increase the level of MOLM. AST decreased significantly (p<0.05) while, ALT did not affect with adding MOLM levels. Thyroid hormones (T3 and T4) were significantly (p<0.05) increased with increase MOLM level while, T3/T4 ratio did not affect. It could be concluded that addition of Moringa oleifera leaves meal up to 0.3% improved broiler performance, physiological parameters and enhanced the ability to resist heat stress conditions of broilers fed corn-soybean meal diet.
  H.M.A. Hassan , A. Samy , A.E. El-Sherbiny , M.A. Mohamed and M.O. Abd-Elsamee
  Background: The environmental issues related to the presence of phosphorus (P) in poultry excreta have led the researchers to manipulate the diet of poultry in order to decrease the P excretion without having any negative impact on the performance of birds. Presently, added minerals are used as nanoparticles in order to increase absorption and subsequent decreased presence in poultry excreta. Therefore, an experiment was conducted to study the effect of dietary nano-dicalcium phosphate (NDCP) compared to conventional dicalcium phosphate (CDCP) on performance and excreted calcium (Ca) and P in broiler chicken. Materials and Methods: Two hundred and eighty one day-old male broiler chicks were divided into seven treatment groups for a period of 26 days. Seven experimental diets were formulated having three levels of either CDCP or NDCP at 1.75, 1.31 and 0.88% and a lower level of NDCP at 0.44%. Thus, these diets contained 100, 75, 50 and 25% of the recommended non-phytate P i.e., 0.45%. The diet having 1.75% CDCP (100% recommended non-phytate P) served as a control diet. Every dietary treatment had 4 replicates of 10 chicks each. Broiler performance, Ca and P excretion were studied. Results: Birds fed different levels of NDCP gained significant more body weight (p<0.05) and utilized feed more efficiently than the control group (1.75% CDCP). Decreasing levels of CDCP led to decrease in body weight gain and impaired feed conversion ratio compared to the control group. Values of body weight gain and feed intake increased by about 25 and 10%, respectively, feed conversion ratio improved by about 12% for birds fed NDCP compared to those fed CDCP. Level of dietary DCP significantly (p<0.001) affected Ca and P excretion while source of DCP significantly (p<0.001) affected P excretion but had no effect on (p>0.05) Ca excretion. Feeding 0.44% NDCP in the diet decreased the excreted Ca and P by 50.74 and 46.24%, respectively, compared to the control. Conclusion: It could be concluded that using NDCP in broiler diets allow successfully to reduce the dietary DCP by 75%. Diet formulated containing only 25% of the required non phytate P in form of NDCP could be used instead of 100% CDCP. Also, using dicalcium phosphate in nanoparticle size allow to reduce the excreted Ca and P by about 50% which reduce the impact of poultry on environmental pollution.
  M.A. Mohamed , H.M.A. Hassan , A. Samy , M.O. Abd-Elsamee and A.E. El-Sherbiny
  Background: A broiler experiment was carried to study the effect of using nano dicalcium phosphate (NDCP) compared with the conventional dicalcium phosphate (CDCP) on carcass characteristics and bone measurements. Materials and Methods: Seven groups of one day-old (Ross 308) male broilers were fed on seven experimental diets. Diets were formulated to contain three levels of CDCP or NDCP being, 1.75, 1.31 and 0.88% and a less level of NDCP being 0.44%. These levels supplied 100, 75, 50 or 25% of the recommended dietary available P requirement, correspondingly. Diet of 1.75% CDCP served as a control. The Ca: P ratio was kept 2:1 in all the diets. At the 26th day of age, carcass characteristics and tibia bone parameters were measurements. Results: No significant differences were detected on liver, heart and gizzard weights (% of live body weight) among all treatments while carcass weight represents live body weight. Using NDCP instead of CDCP showed significant (p<0.001) increase in the measured bone parameters. Birds fed 0.44% dietary NDCP showed comparable values of tibia weight, length, width and breaking strength as those fed 1.75% CDCP. The NDCP increased tibia ash, Ca and P% by 4.61, 3.62 and 4.28%, respectively, compared to CDCP. The results of bone mineral density reflected the values obtained for tibia ash, Ca and P%. Conclusion: It could be concluded that using NDCP instead of CDCP improved all the measured bone parameters. Diets formulated containing only 25% of the required available P level in form of NDCP could be used instead of using 100% of the requirements in form of CDCP. The dietary dicalcium phosphat level could be successfully decreased from 1.75-0.44% when used in form of nano particle size. Dicalcium phosphate in nanoparticle size was of about 400% as available as the conventional dicalcium phosphate.
  M.O. Abd-Elsamee , A.E. El-Sherbiny , H.M.A. Hassan , A. Samy and M.A. Mohamed
  A broiler growth experiment was conducted using 360 one-day old Ross 308 chicks to study the effect of reducing dietary phosphorus and adding phytase enzyme. Two diets were formulated in starting period (1-20 days): a control diet contained 0.50% Available Phosphorus (AP) and a low P diet contained 0.40% AP. Such low P diet was fed without or with phytase supplementation (500 IU kg-1). At the growing period (21-35 days) every group of birds of the first period was divided into two sub-groups. Two grower diets were formulated, a control diet contained 0.40% AP and a low P diet contained 0.30% AP. The low AP diet was offered with phytase supplementation (500 IU kg-1). Growth performance, bone parameters and P excretion were measured at 20 and 35 day of age. No significant differences on chick performance among dietary treatments during the starting and growing periods were observed. The best FCR value was recorded for birds fed 0.50% AP diet in the first period then grown on 0.30% AP diet+phytase. Reducing dietary AP content did significantly (p<0.05) affect bone parameters at 20 and 35 day of age. Addition of phytase did alleviate such effect. Tibia Ca and P content significantly (p<0.001) increased by phytase supplementation. Phosphorus excretion decreased more than 20% at staring period and more than 30% at growing period when broilers were fed low P diets supplemented with phytase. The results showed pronounced beneficial effect regarding the excreted P. Supplementing phytase enzyme to broiler diets renders the dietary phosphorus contents more available to the birds. Therefore, the amount of supplemental phosphorus could be remarkably reduced. It could be concluded that reducing dietary P level and using phytase enzyme could limit quantity of excreted P from broilers without adverse effect on performance. This reduces such impact in environmental pollution.
  Amani W. Youssef , H.M.A. Hassan , H.M. Ali and M.A. Mohamed
  This study was conducted to evaluate the effect of various commercial feed additives on performance and egg quality of laying hens. These additives included probiotics (Protexin® and Clostat®), symbiotic (Diamond®) and organic acids (Galliacid®). A number of 180 HL Brown hens (27 wks of age) were divided into 5 treatment groups (6 replicates of 6 birds, each). Groups were assigned to 5 experimental diets: a basal diet of no additive (control), the basal diet supplemented with either 0.01% Protexin®, 0.05% Clostat®, 0.06% Diamond® or 0.06% Galliacid®. Data of layer performance and egg quality were obtained during 12 weeks experimental period. Supplementation of probiotics or symbiotic recorded higher (p>0.05) egg production than the control but organic acids supplementation significantly (p<0.05) increased egg production by 9.94%. Egg weight slightly improved (p>0.05) by dietary treatments. Supplementation of probiotics, symbiotic and organic acids significantly (p<0.01) increased egg mass. The best egg mass value was recorded for birds fed diet supplemented with organic acids. Feed conversion ratio improved (p>0.05) by dietary treatments. Adding probiotics, symbiotic or organic acids did not significantly affect shape index, yolk index, yolk %, SWUSA, Haugh unit or specific gravity. Addition of probiotics or organic acids showed significant (p<0.05) increase in shell thickness and yolk color. It could be concluded that these additives caused improvement in performance and egg quality of laying hen. More studies are needed to explain the effects of different sources and levels of these additives on performance and egg quality of laying hens.
  H.M.A. Hassan , Amani W. Youssef , Eman F. El- Daly , Nafisa A. Abd El- Azeem , Eman R. Hassan and M.A. Mohamed
  An experiment was designed to evaluate the effect of using three different commercial preparations of Direct-Fed Microbials (DFM) being: A mixture of Enterococcus faecium (Protexin®, DFM1), a mixture of Bacillus subtilis (Clostat®, DFM2) and Saccharomyces cerevisiae yeast cells with its fermentation metabolites (Diamond®, DFM3) on performance, carcass characteristics, caecum bacterial count and changes on ileum histology of broilers. A number of 200 Cobb 500 broiler chicks were fed on 4 dietary treatments from 10-36 days of age: Abasal corn-soybean meal diet served as a control treatment with no supplements or supplemented with the recommended levels of the tested products. No significant differences were detected on Body Weight Gain (BWG) among treatments. Chicks fed the control diet consumed more (p<0.05) feed than those fed DFM supplemented diets. No significant differences on Feed Intake (FI) were observed among birds fed the different examined DFM. Birds fed the control diet recorded the worst values (p<0.01) of Feed Conversion Ratio (FCR) among the dietary treatments. Addition of DFM to broiler diets improved efficiency of feed utilization (FCR values) by about 6%. Addition of DFM to broiler diets decreased Escherichia coli and Clostridium spp. count in the ceacum. Also, feeding DFM supplemented diets stimulated histological changes in the ileum villi (height and width along with the number of crypts). These changes reflected a positive effect of the tested products on intestinal efficiency which may explain the improvement in FCR of broilers. It could be concluded that birds fed DFM supplemented diets utilized feed more efficiently than those fed the control diet. The DFM that contain Enterococcus faecium, Bacillus subtilis or Saccharomyces cerevisiae yeast cells with its fermentation metabolites, exert the same effects upon the studied parameters.
  A.E. El-Sherbiny , H.M.A. Hassan , M.O. Abd-Elsamee , A. Samy and M.A. Mohamed
  An experiment was conducted using 300 growing Ross 308 broilers from 23 to 40 days of age. Six dietary treatments were formulated. A basal diet contained 1.48% Dicalcium Phosphate (DCP) was used as a control diet (diet 1). Diets 2 and 3 contained 50% and 25% of the DCP of diet 1 (0.74 and 0.37%, respectively), while diet 4 was formulated without DCP. Diets 3 and 4 were fed without or with supplemented phytase enzyme (500 U/kg). Every dietary treatment was fed to 5 replicates (10 chicks each). The results showed no significant differences between birds fed diets containing 1.48 % DCP or 0.74% DCP on BWG, FI and FCR. Reducing dietary DCP level to 0.37% slightly decreased BWG compared with birds fed 0.74% DCP with inferior value of FCR. When DCP was removed from the diet BWG significantly (p<0.01) decreased and FCR recorded worth value. Addition of 500 U phytase/kg to diet 3 of 0.37% DCP significantly enhanced BWG (p<0.01), feed intake and FCR (p<0.05). Addition of phytase to the diet of no DCP did improve neither BWG nor FCR. Decreasing dietary DCP did not significantly affect length, weights and width of tibia either with or without phytase supplementation. However, birds fed the highest level of dietary DCP showed the highest values of tibia weight and length among the different groups. Tibia breaking strength (kgf) significantly (p<0.001) decreased as dietary DCP level decreased. Addition of phytase significantly (p<0.001) improved tibia breaking strength and tibia ash %. Addition of phytase to diet of low DCP did increase tibia Ca and P to reach values comparable with those of the control diet. Decreasing dietary DCP showed significant (p<0.001) decrease in the excreted Ca and P. Addition of phytase to diets of low or no DCP also decreased (p<0.001) the excreted Ca and P. This means that phytase increased the utilization of dietary Ca and P. The excreted Ca and P decreased by 41.22% and 55.26%, respectively, when birds were fed diet of no DCP compared to those fed the control diet. Also, addition of phytase enzyme to diets of low or no DCP decreased the excreted percentage of Ca and P. It could be concluded that reducing dietary P level and using phytase enzyme could be used to limit quantity of P excreted from broilers. This reduce such impact in environmental pollution.
  M.A. Mohamed , Eman F. El- Daly , Nafisa A. Abd El- Azeem , Amani W. Youssef and H.M.A. Hassan
  An experiment was designed to evaluate the effects of using organic acids as an alternative to antibiotic growth promoter on performance of broiler chicks. Carcass characteristics, histological changes of ileum and immune related organs (bursa, thymus and spleen) along with intestinal bacteria count were also studied. A number of 150 Cobb broiler chicks were fed on three dietary treatments: a basal corn-soybean meal diet served as a control treatment with no supplements or supplemented with either 0.025% Bacitracin Methylene Disalicylat (BMD, antibiotic) or 0.06% Galliacid® (organic acids) from 10 to 36 days of age. The results showed that birds fed antibiotic or organic acids gained significant (p<0.05) more body weight than those fed the control diet. No significant differences were detected among treatments on feed intake while feed conversion ratio (FCR) values were significantly (p<0.001) differ. Addition of organic acids or antibiotic did improve FCR by about 9 and 4%, respectively. These results indicated that birds fed either organic acids or antibiotic supplemented diets utilized feed more efficiently than those fed the control diet. Carcass characteristics were not affected by dietary treatments, while the addition of antibiotic or organic acids significantly (p<0.01) increased spleen and bursa weight (% live body weight). Addition of organic acids was more effective than antibiotic on decreasing intestinal count of Escherichia coli and appearance of Colostridium perfringers. Organic acids as alternative to the antibiotic growth promoter have stimulated some histological change in histology of the villi and the immune related organs. Performance and feed efficiency are closely interrelated with the quantitative microbial load of the gut, the morphological structure of the intestinal wall and the activity of the immune system. In conclusion, dietary inclusion of organic acids increased growth performance and improved intestinal health and morphology of broiler chicks. It could be successfully used as alternative to antibiotic growth promoters in broiler diets or as a tool of controlling intestinal pathogenic bacteria.
  Nafisa A. Abd El-Azeem , Eman F. El-Daly , H.M.A. Hassan , Amani W. Youssef and M.A. Mohamed
  An experiment was conducted to study the effect of feeding different commercial preparations of direct-fed microbials (DFM) used as growth promoters on weight and histology of immune related organs (bursa, thymus and spleen) in broilers. Two hundred unsexed 10 days old Cobb broiler chicks were individually weighed and divided into 4 groups (5 replicates of 10 chicks, each) and fed 4 different experimental diets. A mixture of Enterococcus faecium (Protexin®, DFM1), a mixture of Bacillus subtilis (Clostat®, DFM2) and Saccharomyces cerevisiae yeast cells with its fermentation metabolites (Diamond®, DFM3) were supplemented to broiler diets and compared with the basal diet which served as control. Bursa, thymus and spleen were taken from birds at 36 days of age. The results showed significant (P<0.01) increase in spleen and bursa weight (relative to live body weight) in birds fed DFM supplemented diets compared with those fed the control diet of no supplement. Addition of DFM enhanced the activity of bursal follicles and may improve the bursa activity and caused improvement in thymus and spleen structure compared with the control. In conclusion, DFM supplemental levels have stimulated some histological change in the immune related organs which may result in improvement of chick immunity.
  M.A. Mohamed , H.M.A. Hassan , Amani W. Youssef and Sawsan M. Gad
  Growth experiment was carried out to examine the effect of feeding broilers on finisher diet (23 to 39 days of age) contained low levels of trace mineral premix. Two hundred male Ross broiler chicks were divided into four treatment groups, five replicates each. Four corn-soybean finisher diets were formulated to cover all the nutrient requirement and the trace mineral premix (TMP) was added at 100, 75, 50 or 25% of the recommended level. Performance, carcass characteristics, tibia ash, mineral excretion and economic efficiency were measured. The results showed that reducing trace mineral content in finisher diets did not affect chick performance (p>0.05). There were no significant differences in body weight gain, feed intake and feed conversion ratio. Also, carcass characteristics and tibia ash content did not significantly affect by lowering level of dietary TMP. Mineral excretion significantly (p<0.05) decreased with reduced trace mineral levels. Economic efficiency slightly improved with reducing dietary trace mineral content. It could be demonstrated that it is possible to reduce level of dietary TMP in finisher broiler diets with no adverse effects on performance, carcass characteristics or tibia ash content. It could help not only to maintain general performance, but also to reduce any potential environmental pollution for sustainable poultry production.
  H.M.A. Hassan , A. Samy , Amani W. Youssef and M.A. Mohamed
  Background and Objective: Several feed additives have been used to improve feed efficiency and growth performance of broiler. This growth experiment aimed to study the effect of using different feed additives compared to Bacitracin Methylene Disalicylate antibiotic growth promoter on growth performance and carcass traits. Methodology: Three hundred broiler chicks were divided into six treatment groups (5 replicates, 10 birds each treatment). A basal corn-soybean meal diet was formulated. Treatment 1 was basal diet without additives (as a control group); treatments 2, 3, 4, 5 and 6 were the control diet supplemented with 0.025% Bacitracin Methylene Disalicylate (antibiotic), 0.05% Saltose (probiotic), 0.05% Clostat (probiotic), 0.05% Clostri-stop (probiotic) or 0.1% Sangrovit (phytobiotic), respectively. Results: The results showed that supplementation of different feed additives or antibiotic significantly (p<0.001) improved body weight gain (BWG) and feed conversion ratio (FCR) in the finisher period (from 26-35 days of age) and the overall period (1-35 days) compared with the control (without additives). Results of carcass traits showed that there was a significant (p<0.05) increase in carcass weight and dressing percentage of broilers fed antibiotic or feed additives supplemented diets compared to those fed the control diet. However, internal organs were not affected by supplementation. Conclusion: Using probiotics or phytobiotics in broiler diet as feed additives appeared to be superior compared to antibiotic growth promoter. It could be concluded that, addition of feed additives containing Bacillus sp., Clostridium butyricum (probiotics) or Sanguinarine (phytobiotics) to broiler diets could significantly improve growth performance and carcass traits more efficiently and safely than antibiotic growth promoter. These could be good alternatives to antibiotic growth promoters in broiler diets.
 
 
 
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