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Articles by H.M. Salleh
Total Records ( 3 ) for H.M. Salleh
  I.A. Noorbatcha , N. Samsudin and H.M. Salleh
  Phytases catalyze the hydrolysis of inorganic phosphate from phytic acid and are able to improve the nutritional quality of phytate rich diet. Monogastric animal such as poultry and fish have lack of significant activity to hydrolyze phytate that contribute to elimination of beneficial nutrient for growth therefore contribute to land pollution, eutrophication of ground water and aquatic environment. Besides, it leads to the negative effect on vitamin utilization that lead to the emaciation, retarded growth and reproductive failure to animal. Due to the importance of, microbial sources for the commercial production of phytases, we have selected waste water bacterium phytase as the subject of interest in this study. In silico experiment is used to identify and examine the active site of waste water bacterium phytase. The factors influencing the ligand binding strength in the active site is analyzed and computational site directed mutagenesis experiments were carried out to evaluate the effects of mutations on the binding strength. Multiple mutations of M216R/E219R/H17A, M216R/E219R/F254E and some other multiple mutations showed improvement in the binding strength, primarily due to the addition of hydrogen bond with the adjacent residues. Automated docking based on genetic algorithm is used to dock the phytate in the active site and Partial Mean Force (PMF) scoring is used to calculate the strength of the binding before and after mutation.
  I.A. Noorbatcha , M.A. Hadi , A.F. Ismail and H.M. Salleh
  The use of hemicellulolytic enzymes has recently attracted considerable interest as a substitute for chlorine chemicals in pulp bleaching. The challenges in incorporating enzyme to the bleaching system are to have a stable and active xylanase at high temperatures and higher alkaline pH conditions. In this study, we have used computational methods to analyze the structural factors responsible for the activity and themostability of Bacillus circulans xylanase which have been identified as one of the promising xylanase source to treat the pulp before bleaching it through the conventional bleaching sequences. Simulated point mutation shows that arginine substitution potentially increased the number of hydrogen bond; correlate with the xylanase activity and some changes in amino acid sequence specifically to the α-helix and β-sheet appeared to be promising in improving thermostability of xylanase. This in silico method results can be used to develop new efficient xylanase for pulp bleaching industry.
  M. Mel , M.I.A. Karim and H.M. Salleh
  This study is focused on the evaluation of the volumetric mass transfer coefficient (kLa) of E. coli fermentation producing β-glucuronidase enzyme in different bioreactor operating conditions. The operating conditions used were according to the Taguchi’s method design using three factors of fermentation viz., temperature, agitation speed and air flow rate at two concentration levels. From the four experiments conducted, experimental run number two with agitation of 300 rpm, airflow rate of 2 vvm and temperature 41°C generate the highest Optical Density (OD 660 nm) of 2.313 for cell count and also having an equivalent kLa value of 0.0281 min-1 or 1.686 h-1. This condition was found to be an effective point for upscaling in bigger bioreactor for E. coli fermentation to produce the enzyme.
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