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Articles by H.L. Zhou
Total Records ( 4 ) for H.L. Zhou
  S. Guan , H.L. Zhou , G.Y. Hou , D.J. Wang , T.S. Xu , D.F. Wang , L.G. Shi , G. Rong and M. Li
  This study investigated the H-FABP mRNA expression levels in Hainan Black goats. Males from 1-4 months of age beyond adult animals were selected. Three animals of each age were slaughtered to collect samples from heart skeletal muscle (Longissimus dorsi muscle, psoas major muscle, biceps femoris muscle) adipose tissue liver and kidney. The total RNA was obtained to investigate the H-FABP mRNA expression levels by real-time PCR. The results showed that the H-FABP mRNA expression levels in heart and skeletal muscle have the same basic trends at different growth periods. The highest H-FABP mRNA expression in heart and skeletal muscle appeared at 2 months of age and it was extreme higher than those at other ages (p<0.01). The lowest H-FABP mRNA expression level in these tissues were at 1 month old. H-FABP mRNA expression level in adipose tissue was different from those in heart and skeletal muscle. The highest H-FABP mRNA expression in adipose tissue appeared at adult age and its expression was extreme higher than those in younger ages (p<0.01). In addition, the H-FABP mRNA expression pattern in liver was different from the other tissues. The expression in the liver at 1 month of age was significantly higher than those at 2-4 months of age. However, the highest level occurred at adult age. The differences in H-FABP mRNA expression level at 3 and 4 months old were not significant in liver, heart, skeletal muscle and adipose tissue. On the other hand, H-FABP mRNA expression level in heart was the highest and significantly higher than other tissues at all ages. But, H-FABP mRNA expression level in liver was extreme higher than those in skeletal muscle and adipose tissue at 1 month old age (p<0.01). Besides, H-FABP mRNA expression in adipose tissue appears to increase as the age increase.
  S. Guan , H.L. Zhou , G.Y. Hou , D.J. Wang , T.S. Xu , D.F. Wang , L.G. Shi , G. Rong and M. Li
  Phylogenetic and genetic diversity of four local goat breeds were analyzed by mitochondrial Hypervariable Region (HVR) in 89 individuals from Chinese tropical zone. A total of 36 haplotypes which defined 64 polymorphic sites were found in the study. Comparing with the published mtDNA control region sequences, two mtDNA lineages (A and B) were identified by the phylogenetic analysis in which lineage A was 57.30%, lineage B was 42.70%, haplotype A was 47.22%, haplotype B was 36.11%. The interest in the study was that the proportion of lineage B was up to 80.95% in Hainan black goat, lineage A was only 19.05%. The genetic diversity showed that Hainan black goat had the lowest variability. The average diversity and nucleotide diversity were 0.957±0.007 and 0.02887±0.00256, respectively. A mantel test and the Analysis of Molecular Variance (ANOVA) indicated that there was no significant geographical structuring in Chinese tropical zone goat breeds. Mismatch analysis showed that haplogroup A and B had not experienced population expansion events. According to the study and previous evidence, researchers speculated that lineage B of goat breed might originate from Hainan Island of China.
  T.S. Xu , J.B. Liu , D.W. Yao , H.F. Cai , H. Chen , H.L. Zhou and X.Y. Lan
  Due to the significant influence of GH, PRL and PIT1 mutations on production traits, Prophet of PIT1 (PROP1) regulating them is considered as an important and potential candidate gene for goat growth traits by Marker Assisted Selection (MAS). Using Hin6I PCR-RFLP, this study verified the AF453512: g.1795C >T resulting in GCG (79Ala)>GTA (79Val) within PROP1 gene in Hainan Black Goat (HNBG). Three genotypes (CC, CT and TT) were observed and their frequencies were 0.310, 0.493 and 0.197, respectively. The allelic frequencies of allele C and T were 0.556 and 0.444, respectively in analyzed population with Hardy-weinberg equilibrium (p>0.05). Combining with the reported genotypic frequencies at Hin6I locus in cashmere goat breeds (IMWC and SBWC), dairy goat breeds (Sa, LS and GZ), genotypic frequency of HNBG population showed significant differences when compared with other goat breeds (p<0.001) as well as significant differences of allelic frequencies between HNBG and other breeds were revealed (p<0.001), implying that the allele T possibly was associated with the meat traits and growth traits. Association analysis showed significant relationships between Hin6I polymorphisms and Body Length (BL), Chest Circumferene (ChC), Chest Width (ChW) and Cannon Circumference Index (CaCI) ((p<0.05), suggesting that the PROP1 gene produced significant influences on growth trait. The individuals with genotype CT demonstrated better Body Length (BL), Chest Circumferene (ChC) and Cannon Circumference Index (CaCI) (p<0.05) when compared with those of individuals with genotype CC.
  H.L. Zhou , S. Guan , G.Y. Hou , D.J. Wang , T.S. Xu , D.F. Wang , L.G. Shi , G. Rong and M. Li
  The genetic diversity and relationships of Hainan Black goat breed among 8 populations were studied using 14 microsatellite markers. In the 14 microsatellite loci, the number of alleles per locus ranged from 15 (BMS1248) to 44 (BMS2258) and the Ho values ranged from 0.003 (BM6404) to 0.923 (BM6444) among the 8 groups, the mean Polymorphic Information Content (PIC) of all the primers detected for all groups showed high values (0.892). For all the goat groups, the mean number of alleles ranged from 9.577 (Baisha) to 16.571 (Saya), the average observed heterozygotes, expected heterozygosity, Polymorphism Information Content (PIC), allelic richness and effective number of alleles was 0.402, 0.866, 0.838, 7.625 and 12.425, respectively. Most of the 14 loci were in Hardy-Weinberg equilibrium (p>0.05) except locus BMS2258 (p<0.0001) and all the 8 populations were in Hardy-Weinberg equilibrium (p>0.05) too. The percentage of variation among the 8 goat groups only amounted to 5.11%, the remaining existed among individuals within populations (58.12%) and within individuals (36.77%). The genetic differentiation pattern and genetic relationships among the 8 populations displayed some degree of consistency with their geographical locations expect Sanya and Linshui regions and suggested that exist a certain inbreeding between SY and CM. The Fis indicated some degree inbreeding existed in Changjiang goat group and there was no significant among the 8 populations. This study will help to interpret the genetic characters of Hainan Black goat and benefit to the future conservation programs.
 
 
 
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