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Articles
by
H.K. Lim |
Total Records (
2 ) for
H.K. Lim |
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H.K. Lim
,
K. Choi
,
P.K. Mandal
,
O. Baatartsogt
,
C.H. Lee
,
J.H. Lee
and
H.B. Kim
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This study was carried out to investigate the differentially
expressed genome between S. gallinarum infected and uninfected
control in the spleen lymphocytes of Ross broiler chicks using microarray
analysis. GeneChip Chicken Genome Array containing 32,773 transcripts
corresponding to over 28,000 chicken genes for simultaneous expression
was used. The signal intensity of each gene was normalized and expressed
in fold change. A large numbers of genes were found with differential
expression majority of which are still unknown in chicken genome. Thirty
one known genes were found to have differential expression of which, 25
were up-regulated and 5 were down regulated. Majority of the up-regulated
genes belong to immune response system viz., IL8, IL1B, IL10, IL18,
IL17A, IL15, transferrin, IFNg, TLR2, TNFRSF1b, TNFRSF15 and the down
regulated genes were B-FIV, B-LA, SDF1, B-LBI, belonging to MHC-I and
II and CD1d. To validate the expression of these genes RT-PCR was done
using primers of 12 selected genes′ with total mRNA isolated from
spleen lymphocytes which has confirmed the similar pattern of expression
of all the genes as in microarray. The findings in this study have lead
to the identification of novel genes which may be useful in further studies
to understand the patho-physiology of fowl typhoid towards development
of diagnostics and therapeutics. |
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O. Baatartsogt
,
K. Choi
,
P.K. Mandal
,
H.K. Lim
,
J.D. Oh
and
S.H. Chang
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This research was done to study the protein expression
profile of synovial cells of knee using proteome analysis during the development
of experimental Post-Traumatic Arthritis (PTA) in swine. PTA was induced
by transection of the Anterior Cruciate Ligament (ACL) of left knee in
three piglets. Articular cartilage and synovial tissues were obtained
after 0, 2, 5 and 8 weeks for histopathologic examination. After sacrificing
the piglets at 8 weeks, synovial tissues were collected for 2-dimensional
electrophoresis and mass spectrometric analysis. Histopathologic examination
at 8 weeks showed overt chronic inflammation indicating the development
of ETA. Through proteome analyses more than 1,500 protein spots were identified
in which 7 differentially expressed protein spots were observed in ACL-transacted
synovial tissue. Five proteins were down-regulated (cytoskeletal β
actin, cofilin-1, destrin, Rho GDP dissociation inhibitor α and an
unnamed protein product) and two proteins were up-regulated (α-B
crystalline and Smooth Muscle Protein (SMP) 22α). These results showed
that proteins that are related to cellular organization and signal transduction
are down-regulated and those that are related to cell rescue, defense
and stress are up-regulated. Therefore, the proteome analysis of synovial
tissue provided us new candidate molecules which may be useful to understand
the pathogenesis for diagnostic and therapeutic studies of Post Traumatic
Arthritis (PTA). |
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