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Articles by H.K. Lim
Total Records ( 2 ) for H.K. Lim
  H.K. Lim , K. Choi , P.K. Mandal , O. Baatartsogt , C.H. Lee , J.H. Lee and H.B. Kim
  This study was carried out to investigate the differentially expressed genome between S. gallinarum infected and uninfected control in the spleen lymphocytes of Ross broiler chicks using microarray analysis. GeneChip Chicken Genome Array containing 32,773 transcripts corresponding to over 28,000 chicken genes for simultaneous expression was used. The signal intensity of each gene was normalized and expressed in fold change. A large numbers of genes were found with differential expression majority of which are still unknown in chicken genome. Thirty one known genes were found to have differential expression of which, 25 were up-regulated and 5 were down regulated. Majority of the up-regulated genes belong to immune response system viz., IL8, IL1B, IL10, IL18, IL17A, IL15, transferrin, IFNg, TLR2, TNFRSF1b, TNFRSF15 and the down regulated genes were B-FIV, B-LA, SDF1, B-LBI, belonging to MHC-I and II and CD1d. To validate the expression of these genes RT-PCR was done using primers of 12 selected genes′ with total mRNA isolated from spleen lymphocytes which has confirmed the similar pattern of expression of all the genes as in microarray. The findings in this study have lead to the identification of novel genes which may be useful in further studies to understand the patho-physiology of fowl typhoid towards development of diagnostics and therapeutics.
  O. Baatartsogt , K. Choi , P.K. Mandal , H.K. Lim , J.D. Oh and S.H. Chang
  This research was done to study the protein expression profile of synovial cells of knee using proteome analysis during the development of experimental Post-Traumatic Arthritis (PTA) in swine. PTA was induced by transection of the Anterior Cruciate Ligament (ACL) of left knee in three piglets. Articular cartilage and synovial tissues were obtained after 0, 2, 5 and 8 weeks for histopathologic examination. After sacrificing the piglets at 8 weeks, synovial tissues were collected for 2-dimensional electrophoresis and mass spectrometric analysis. Histopathologic examination at 8 weeks showed overt chronic inflammation indicating the development of ETA. Through proteome analyses more than 1,500 protein spots were identified in which 7 differentially expressed protein spots were observed in ACL-transacted synovial tissue. Five proteins were down-regulated (cytoskeletal β actin, cofilin-1, destrin, Rho GDP dissociation inhibitor α and an unnamed protein product) and two proteins were up-regulated (α-B crystalline and Smooth Muscle Protein (SMP) 22α). These results showed that proteins that are related to cellular organization and signal transduction are down-regulated and those that are related to cell rescue, defense and stress are up-regulated. Therefore, the proteome analysis of synovial tissue provided us new candidate molecules which may be useful to understand the pathogenesis for diagnostic and therapeutic studies of Post Traumatic Arthritis (PTA).
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