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Articles by H.H. Musa
Total Records ( 4 ) for H.H. Musa
  H.H. Musa , B.C. Li , G.H. Chen , T.P. Lanyasunya , Q. Xu and W.B. Bao
  Traditional karyotyping is invented in animal research for several decades depend on the analysis of characteristic banding patterns along the length of chromosome. In the present study chicken metaphase chromosomes were obtained by peripheral blood lymphocyte culture techniques, G-band patterns were obtained with trypsin and Giemsa, C-band patterns were treated with barium and the nuclear organizer regions (NORs) were identified by silver staining. All species studied presented a diploid number of 78 chromosomes, with 10 pairs of macro chromosomes including the sex chromosome and 29 pairs of micro chromosomes. G-band patterns were found quite different between breeds. The dark stained of C-band was observed on micro chromosome and W chromosome. Karyotype resemblance near coefficient was possible for breeds clustering. The position of centromers, relative length, arm ratio and the evolutionary distance of chicken breeds was estimated. The application of chromosome karyotype and banding techniques was used to study the origin, evolution and relationship of species, also used for gene location and sex determination. While, in the Medical field was used to identify genetic disease. The techniques was consider as a base for further molecular research, for example FISH.
  X.S. Wu , H. Wu , B.C. Li , G.Y. Zhou , S.Y. Sun , J. Qin , G.H. Chen and H.H. Musa
  To isolate, purify and culture spermotogonia from chicken testicular tissues, a procedure of enzymatic digestion and percoll density centrifugation was adopted for the single cell suspension to obtain purified spermatogonia. The results showed that, using the same purification method, the purity of spermatogonia gained from 6 days old chicken embryo was more than from 13, 15 and 19 days of age; adhesion purification step led to a harvest of 82% of total spermatogonia, which was 15.6% higher than that of direct isolation method; the adhesion time and survival time of spermotogonia before percoll density gradient centrifugation was earlier and longer than after precoll density gradient centrifugation.
  H. Musa , I.A Yakasai , H.H. Musa , K.Y Musa and M.S. Gwarzo
  The purpose of this study was to investigate whether the level of inorganic chemical cadmium (Cd) in wells and boreholes water in Zaria is sufficient to affect the health of the inhabitants of the area under study. Sixty open wells and 5 boreholes were randomly selected from Zaria and environs. Water samples were collected from the open wells and boreholes using standard techniques. In the study, Cd concentrations of the samples was determined using standard procedures. WHO acceptable limit for drinking water was used in the evaluation. Result obtained shows that Cd concentrations ranged from <0.001 to 0.28 mg L-1 with 68% of the samples above the WHO water guideline. Most of the open wells and boreholes water studied were contaminated with abnormal concentration of cadmium sufficient enough to affect the health of the inhabitants of the area.
  H.H. Musa , J.H. Cheng , X.S. Wu , H.P. Ju , D.M. Mekki and G.H. Chen
  Present study was focus to compare LDL receptor mRNA expression, total cholesterol, triglyceride, lipoprotien levels and abdominal fat weight in genetically fat and lean chickens. Genetically lean (Rugao) and fat (Anka) chickens were reared in the same environmental condition, at 12 weeks of age samples of liver tissue were collected and abdominal fat weight was determined. Similarly, total cholesterol, triglycerides and high density lipoprotein were assayed using a commercial enzymatic kit, very low density lipoprotein and low density lipoprotein were estimated using the Friedewald equation. Total RNA from liver tissues were isolated using the standard Trizol methods and then total RNA was reverse transcribed by moloney murine Leukemia virus. Semi-quantitative RT-PCR was developed to quantify the levels of LDL receptor mRNA expression. The level of LDL receptor expression was significantly (p< 0.05) difference between lean and fat chicken. In addition, lean and fat chickens were significantly differed on triglyceride, very low density lipoprotein and abdominal fat weight. The expression of LDL receptor mRNA in liver of fat chicken was negatively correlated with abdominal fat weight. However, in lean chicken was negatively correlated with total cholesterol, triglyceride, lipoprotein concentration, abdominal fat weight and percentage of abdominal fat weight. In addition, within two breeds LDL receptor mRNA expression in liver was negatively correlated with low density lipoprotein, abdominal fat weight and percentage of abdominal fat weight.
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