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Articles by H.B. Sahib
Total Records ( 4 ) for H.B. Sahib
  A.F.A. Aisha , H.B. Sahib , K.M. Abu-Salah , Y. Darwis and A.M.S. Abdul Majid
  Cytotoxic and anti-angiogenic properties of n-hexane extract of Sandoricum koetjape stem bark were investigated in vitro. 2,3-bis-(2-methoxy-4-nitro- 5-sulfophenyl)-2H-tetrazolium- 5-carboxanilide (XTT) cell proliferation assay was used to study the cytotoxic properties on Human Umbilical Vein Endothelial Cell (HUVEC) and both colon cancer and normal cell lines; HCT-116, HT-29 and CCD-18CO. Rat aorta ring assay was used to study the anti-angiogenic properties of the extract. At 100 μg mL-1, the extract showed 94±5.5% inhibition of the outgrowth of the blood vessels from the rat aorta rings. The extract also showed a dose dependent growth inhibition of all tested cell lines, IC50 values against HCT-116, HUVEC, CCD-18CO and HT-29 were 14, 23, 50 and 52 μg mL-1, respectively. At 50 μg mL-1, the extract had potently induced apoptotic cell death of HCT-116 colon cancer cell line by inducing caspases 3 and 7 activity. These results showed that n-hexane extract of S. koetjape possess both anti-angiogenic and apoptotic properties on colon cancer cell lines making it a good candidate for further studies.
  H.B. Sahib , A.F. Aisha , M.F. Yam , M.Z. Asmawi , Z. Ismail , S.M. Salhimi , N.H. Othman and A.M.S. Abdul Majid
  Angiogenesis is a process by which new blood vessels are formed from the pre-existing blood vessel. Orthosiphon stamineus Benth. OS has been used as a medicinal herb for many centuries. Due to the presence of high level of anti-oxidants and phenolic content compounds in OS and the effect of anti-oxidants and phenolic compounds being anti-angiogenic, the perturbation of new blood vessels ability of OS was tested. Dry powdered leaves of the OS plant were extracted with Petroleum Ether (PE), chloroform (CE), methanol (ME) and water (WE) by using sequential cold maceration method. The ME of OS has the highest anti-angiogenic activity (93.28±1.24%) in the rat aortic assay followed by CE (85.55±1.64%), PE (51.54±4.12%) and WE (50.22±1.23%) in descending order of reactivity. The methanol extract was also found to have potent anti-oxidant activity in the1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay. The IC50 value was measured to be 0.286 mg mL-1. The total phenolic content of 1 mg mL-1 of methanol extract was equal to 38.27%.
  H.B. Sahib , Z. Ismail , N.H. Othman and A.M.S. Abdul Majid
  Estrogen Receptor (ER+) antagonist, Tamoxifen (TMX), is widely used in the treatment of the hormone responsive breast cancer. However, the common occurrence of resistance after prolonged treatment of TMX hampers its effectiveness. Orthosiphon stamineus Benth. (OS) is a common herb found in South East Asia and is used traditionally to treat various types of ailments. The aim of this study was to determine whether the methanolic extract of Orthosiphon stamineus Benth. (MEOS), that had been proven in previous study to act as anti-angiogenic agents, enhance the anticancer efficacy of ER+ antagonists. In this study methanolic extract of (MEOS) was treated to MCF-7 hormone sensitive breast cancer cell line with the addition of TMX. MEOS showed no significant cytotoxic effect towards MCF-7 when used alone, however when combined with TMX, the anti proliferative activity of the combination increased five fold higher when compared to the anti-proliferative activity of singly treated TMX. The result suggests that MEOS synergistically enhance the activity of TMX against hormone responsive breast cancer cells in vitro and may prove to be useful for the treatment of metastatic breast cancer.
  H.B. Sahib , N.A.H. Harchan , S.A.M. Atraqchi and A.A. Abbas
  Angiogenesis, the development of new blood vessels from existing one, is essential in normal growth processes. Uncontrolled angiogenesis is a main contributor to a number of disease states such as asthma, AIDS, bacterial infections, autoimmune disease, cirrhosis, diabetes, obesity, multiple sclerosis and endometriosis, Angiogenesis also considered a key step in tumour growth, invasion and metastasis. Angiogenesis is required for suitable nourishment and removal of metabolic wastes from tumour sites. Therefore, modulation of angiogenesis is considered as therapeutic strategies of great importance for human health. Numerous bioactive plant extracts are recently tested for their anti-angiogenic potential. Among the most frequently studied are plants rich with polyphenols and terpens present in fruits, vegetables and other plants which have high antioxidant compounds. Plant polyphenols inhibit angiogenesis and metastasis through regulation of multiple signaling pathways. Specifically, flavonoids regulate expression of Vascular Endothelial Growth Factor (VEGF), matrix metalloproteinase (MMPs), Epidermal Growth Factor Receptor (EGFR) and inhibit nuclear factor _B (NF_B), phosphatidylinositol-3-kinase (PI3-K/Akt) and Extra Cellular Signal-Regulated Kinases 1 and 2 (ERK1/2) signaling pathways, thereby causing strong anti-angiogenic effects. This review focuses on the antiangiogenic plants.
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