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Articles by H.A. Shalaby
Total Records ( 5 ) for H.A. Shalaby
  M.M. El-Bahy , N.M. El-Bahy and H.A. Shalaby
  Fractionated Haemonchus longistipes crude antigen revealed 7 protein fractions at molecular weights of 93, 74, 67, 56, 32, 26 and 16 kDa. The bands at 56, 32 and 26 kDa reacted specifically with H. longistipes hyper-immune sera prepared in rabbit (1:100 dilution) using EITB technique. The eluted concentrated fractions as well as crude worm antigens were used in diagnosis of natural infection of Gastro-Intestinal Nematodes (GIN) only or associated with other parasites in camels using ELISA technique. H. longistipes crude antigen and fraction of 26 kDa induced high sensitivity in diagnosis of infection in animals harboring GIN only (sensitivity was 95-100%), while it was 85.0-93.3% with 32 kDa fraction and 55.5-73.3% with that of 56 kDa using ELISA technique. The two H. longistipes eluted protein fractions of 26 and 32 kDa showed higher sensitivity than their crude antigens in diagnosis of GIN infection mixed with other parasites. While, Protein bands of 26 kDa appeared more sensitive than the other protein bands in detection of anti- H. longistipes antibodies at higher serum dilution. With special superiority for fraction of 26 kDa, in comparison with the other antigens, sharp specificity or sensitivity in diagnosis, could not be achieved in this study especially in case of mixed infection with other parasites, where these animals might be immune-compromised.
  H.A. Shalaby and F.M. El-Moghazy
  Since the integrity of Toxocara vitulorum cuticle is essential for the nutritive and protective functions, light and scanning electron microscopic studies were undertaken to assess, for the first time, whether the Nigella sativa oil (NSO) had any effect on the cuticle of adult T. vitulorum following incubation in vitro. Differences in response to NSO action were observed, depending on the used concentration. After 24 h incubation with 0.5 mg mL-1 NSO, the cuticle of the anterior end of worms appeared to be slightly more swollen than normal. This swelling became pronounced and so severe, with lips showed wrinkled cuticular surface and deformed sensory papillae on increasing the concentration to 1.0 mg mL-1. With the higher concentration of 1.5 mg mL-1, extensive and severe disorganization of the cuticle and body musculature was observed. Structural alterations in the cuticle as observed in the present investigation were thus, clear indication of nematocidal activity of NSO that could offer a suitable and cheaper alternative for the more expensive anthelmintics.
  N.S. Degheidy and H.A. Shalaby
  A complete understanding of the micromorphological features of the tegument is important in developing any vaccine that can damage the parasite’s tegument. The present study had been carried out to assess tegumental changes in adult Fasciola gigantica recovered from goats after immunization with Glutathione S-Transferase (GST) antigen by means of Scanning Electron Microscopy (SEM). The GST in F. gigantica (FgGST) was isolated by affinity chromatography, by which highly purified enzyme was obtained and evaluated as a vaccine in combination with Freund’s adjuvant. The statistically significant results were obtained when the goats were immunized with GST and challenged with 120 F. gigantica metacercariae; 2 weeks after last immunization, revealing 64.1% worm burden reduction over adjuvant control. Equally important, GST elicited highly significant (p<0.0001) decrease in the size of the recovered flukes. SEM analysis of these flukes revealed evidence of severe swelling of the tegumental surface covering and between the spines in the apical cone and mid-body regions in the majority of the specimens examined. This tegumental swelling showed regional variations in its severity. In extreme cases, there was severe disruption of the apical cone region as well as the spines. These results might raise the possibility of being GST as a prospective vaccine candidate against fasciolosis in goats.
  H.A. Shalaby , F.M. El-Moghazy and F.A.M. Khalil
  In this study, specificity of snail feet and hepatopancreases antigens in antibody detection of their trematode parasite was evaluated via Western blot technique. Snails, of two different families, antigens; Lymnaea cailliaudi as intermediate host of Fasciola gigantica and Biomphalaria alexandrina as intermediate host of Paramphistomum microbothrium, were evaluated in detection of IgG antibodies against their trematode parasites after preparation of the required Hyper-Immune Sera (HIS) in rabbits. The results revealed higher specificity of snail feet in antibody detection than hepatopancreases antigens. Where, three of sex polypeptides of L. cailliaudi feet antigen; identified by F. gigantica HIS, showed specific positive reactivity. These polypeptides were at molecular weights of 59, 57 and 52 kDa. While, one of sex polypeptides of L. cailliaudi hepatopancreases antigen; identified by F. gigantica HIS, at molecular weight of 57 kDa was specific. Similarly, two polypeptides of B. alexandrina feet antigen; at molecular weights of 54 and 45 kDa, showed specific reactivity toward anti-paramphistomum antibodies. At a time, no specific reactivity had been shown by the antigenically active polypeptides of B. alexandrina hepatopancreases antigen. Owing to its cross-reactivity with incompatible trematode parasite, snail antigen can be used as a general starting antigen for immunodiagnosis of trematode infections.
  H.A. Shalaby , S. Abdel- Shafy , H.M. Ashry and F.M. El- Moghazy
  The main goal in the current investigation was to test the effect of a commercial disinfectant, hydrogen peroxide 50% and dihydroxy benzol 100 ppm solution, on the vitality of Toxocara canis eggs aiming to avoid contamination in kennels and veterinarian clinics. The present study added a new advantage to the previously known disinfectant, moreover to the previous disinfection benefits of this mixture. It showed high inhibitory activity on vitality of unembryonated T. canis eggs which depended basically on the tested concentrations. The best effect was observed with 3% concentration after 24 h exposure, where the inhibitory activity was 99.73% and the egg development was arrested at two-cell stage. On the other hand, there were neither morphological changes nor any cessation of the motility of larvae in embryonated T. canis eggs, with all disinfectant concentrations. The present study supported the use of hydrogen peroxide 50% and dihydroxy benzol 100 ppm solution at 3% concentration as a disinfectant agent against T. canis eggs because of its ovicidal effect on unembryonated eggs.
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