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Articles by H.A. Hussein
Total Records ( 10 ) for H.A. Hussein
  H.A. Hussein , H.A. Hussein Ebtissam , O.E. El-Sayed , A.M.F. Al-Ansary , S.A. Khatab and A.A. Rabie Sally
  Grain Protein Content (GPC) of wheat is important for improved nutritional value and is also one of the major factors affecting bread making and pasta quality. This study was an attempt to improve grain protein content in local wheat cultivars by transferring the Gpc-B1 allele from wild accessions of Triticum turgidum ssp. dicoccoides to Egyptian cultivars belonging to two wheat species, i.e., Triticum aestivum (Giza 164 and Sakha 69) and Triticum durum (Beni suif 5 and Sohag 3) using interspecific hybridization. The genetic variability among the studied genotypes (5 T. turgidum ssp. dicoccoides+2 T. aestivum+2 Triticum durum) was assessed using seven agronomic characters, grain protein (GPC), iron (GFeC) and zinc (GZnC) contents. Marker assisted selection using the Xuhw 89 marker was applied to confirm the presence of the Gpc-B1 gene in the wild dicoccoides parental genotypes and the F1 hybrids. Nine out of the twelve hybrids revealed the Xuhw 89 allele of the dicoccoides (122 bp) and showed significant increase in the values of grain protein in addition to one or the two micronutrients (Fe and Zn) compared to the corresponding local cultivars. These values ranged from 88-147, 9-27 and 7-25 mg kg-1, respectively in the hybrids. While, they ranged from 72-97, 8-11 and 7-10 mg kg-1, respectively in the local cultivars. The results revealed that a weak negative correlation exists between grain yield and grain protein content. Nevertheless, some crosses (Giza 164xIrbid, RachayaxGiza 164, Sakha 69xHaifa and Sohag 3xHaifa) exhibited an increase in grain protein content without affecting the grain yield.
  Eman Abo Hatab , H.A. Hussein , I.M. El-Sabagh and M.S. Saber
  We are here reporting the successful isolation and characterization of G10 serotype of group A rotaviruses from fecal samples collected from camel farms suffering from diarrhea in Alexandria and Esmalia Governorates. After preparation of fecal samples and inoculation on MA 104 cell line for five passages, 8 isolates were successfully isolated with a clear and reproducible CPE on the inoculated cells. The isolates were identified antigenically using VP6Monoclonal Antibodies (MAbs) based antigens capture ELISA that able to detect any group A rotavirus. The viral RNA was extracted from the tissue culture harvest of the propagated viruses and RT-PCR using primers specific for VP6 and VP7 of group A rotaviruses was employed and confirmed the molecular characterization of the isolates viruses with the correct and expected bands. The RT-PCR specific band of VP7 gene of two selected isolates was eluted from the agarose gel and sequenced using VP7 specific primers sequence. The obtained sequence was analyzed using computer software (BLAST) which revealed that both isolates had maximum identity to the G10 serotype of group A bovine rotaviruses ranging from 90-93%. This is the first report on the circulation of G10 serotype of group A rotaviruses in camel.
  Jehan A.M. Gafer , H.A. Hussein and I.M. Reda
  The present study was directed to isolate the PI-3 virus from sheep and goats which naturally affected with respiratory manifestation and to identify the isolated virus using several assays. Two hundred and five nasal swab samples were collected from diseased sheep and goats for the isolation of PI-3 virus. The samples were taken from diseased animal at  7  different Governorates Kaffer el sheikh, Alexandria, EL Behaira, Port Said, Demiatta El-Qalubia and Giza through the winter seasons of years 1999 to 2003. The virus was successfully isolated from three ovine samples after three successive passages on MDBK cells. The isolated viruses were then titrated and identified using different biological, serological and molecular assay.
  H.A. Hussein , H.A. Sultan , A.H. EL-Deeb and A.A. El-Sanousi
  Re-emerging of H5N1 severe outbreaks in vaccinated chickens housed in poultry farms in Sharkia governorate in Egypt was observed starting from October 2007 and continued for 3 months until control measures have been taken in such farms, besides multiple vaccination during this period were applied using reassortant H5N1 and 6 types of H5N2 vaccines. In the present study, 9922 serum samples were collected from vaccinated chickens including some broiler breeders and commercial layers from the period of December 2006 to February 2008 and tested for the immune response to the multiple vaccination with different H5N1 and H5N2 avian influenza vaccines (reassortant H5N1 and 6 types of H5N2 vaccines) by Hemagglutination Inhibition (HI) test using heterologous H5 antigen which represent non of the vaccine antigens. The samples were collected from 578 houses mostly commercial layers. H5N1 viral nucleic acid was also detected in swab samples collected from mortalities occurred in some of vaccinated birds after vaccination policy has been applied in Egypt at March 2006. The viral RNAs of the detected H5N1 circulating viruses between Feb. 2006 and Feb. 2008 in Sharkia governorate were sequenced. Results of nucleotide sequence analysis of 11 detected viruses confirm the existence and circulation of the H5N1 in Sharkia governorate along the period of study and no H7 viruses were detected. The serum samples collected from different farms were divided into 4 groups based on the date of collection. Hemagglutination Inhibition (HI )results showed that in the first group (samples collected between December 2006 to March 2007) 43% of the tested samples were of HI titers of 6 log 2 or more which we propose such titer as a protective titer against H5N1 virus. In group 2 (samples collected between April and July, 2007), titers in 36.4% of the tested samples were protective. In group 3 (samples collected between August to November, 2007), titers in 33.2% of the samples were protective. The last group contains samples collected from chicken vaccinated twice during the period of collection (December 2007 to February 2008), besides being previously vaccinated with at least 3 vaccine shots. Hemagglutination Inhibition (HI) titers in such group were protective in 54% of the tested samples although these chickens received 5 doses of H5 vaccines. The present study is highlight the possible multiple causes of re-emergence of H5N1 outbreaks in Egypt.
  H.A. Hussein , M.M. Emara and M.A. Rohaim
  In the early months of 2011, several devastating Newcastle disease outbreaks occurred in Egypt affecting commercial poultry. Both vaccinated and unvaccinated flocks were affected. In the present study, we characterized field isolate of NDV from a broiler flock coinfected with Avian influenza H5N1 showing characteristic clinical signs, post mortem gross lesions and was positive to A.I H5N1 and NDV by RT-PCR. The fusion protein gene of the Newcastle Disease Virus (NDV) isolate was partially amplified by RT-PCR, directly sequenced. The obtained sequence was submitted to GeneBank with the accession No. JX885868 (NDV/chicken/VRLCU138/Egypt/2012). Sequence was aligned and compared with a representative NDV isolates of different genotypes using NCBI BLAST. The F protein cleavage site sequence is a well-characterized determinant of NDV pathogenicity in chickens. The NDV isolate was found to have the motif 112RRQKRF117 which is indicative of the velogenic nature of this NDV isolate. Phylogenetic analysis revealed that then isolated NDV is belonging to subgenotype VIId and in close range to other Chinese VIId NDV isolates based on the high nucleotide similarity between them which indicate that genotype VIId is circulating between chicken flocks. This study reports the characterization of Newcastle Disease Virus genotype VIId in broiler chickens coinfected with Avian influenza H5N1 virus.
  Ayatollah I. Bassiouny , Soad M. Soliman , H.A. Hussein , T.R. Aboelnaga and Ahmed A. EL-Sanousi
  In this study skin lesions were collected from camel suffered from Camelpox Virus (CPV) infection from different region of South Sina Governorate and Maruit camel farm of the Desert Research Center. CPV was isolated and propagated on CAM of 9-11 days SPF-ECE for 10 passages resulting in characteristic pock lesion of the CPV and the highest titer was (4.2 log10EID50 mL-1) at the 7th passage, also CPV was isolated and propagated on Vero cell line for 20 passages resulting in the characteristic CPE in the passage No. 3 after 5 days of inoculation and the virus titer increased gradually till reach (4.7 log10TICD50 mL-1) at the 17th passage. The isolated virus was identified as CPV using Virus Neutrization Test (VNT) with Neutrization Index (NI) equal to 2 and the identification confirmed by using the double antibody sandwich ELISA (DAS-Elisa) on antigen prepared from different virus passages on Vero cell line.
  N.M. Ismail , H.I. Tawfik , H.A. Hussein and I.M. Reda
  Objective of the present study was to investigate the efficacy of different vaccine preparations associating inactivated Avian influenza (H5N2) antigen, Montanide ISA 70 as an adjuvant and flagellin as an immune-enhancer. Their Immunoenhancing effect in chickens after single vaccination dose (0.5 mL) of the prepared vaccines was assessed. Measurement of cell mediated immunity by lymphocyte blastogenesis at 3rd, 7th, 10th, 14th, 21st and 28th was estimated also phagocytic activity was estimated 3rd, 7th, 10th, and 14th days post vaccination. While humoral immune response, based on Haemagglutinin inhibition test was traced 36 weeks post vaccination. The results revealed that vaccination with the inactivated Avian influenza (H5N2) vaccine in combination with Montanide oil ISA 70 provides robust immunity with longer duration and the addition of flagellin as immune-enhancer ensures a significant higher antibodies titer and better cellular immune response than those vaccines not combined with flagellin.
  A.I. Bazid , H.A. Hussein , S.S. Balal , A.A. ELsanousi and B.M. Ahmed
  This study describes the sequence analysis and molecular characterization of foot-and-mouth disease virus type O responsible for FMD outbreaks in Egypt in 2009. Phylogenetic analysis of partial VP1 nucleotide sequences demonstrated that the examined FMDV type O was related to Pan Asia strain within ME-SA topotype, rather than to O1 serotype used in vaccine preparation in Egypt.
  Ayatollah I. Bassiouny , Soad M. Soliman , H.A. Hussein , M.A. Rohiam and Ahmed A. EL-Sanousi
  In this study the collected skin sample from camel suffered from Camelpox virus (CPV) from different regions in Egypt (South Sina Governorate and Maruite camel Farm of the Dessert Research Center) and the propagated isolated virus on Vero cell line were characterized by employing Polymerase Chain Reaction (PCR) and sequencing. The causative agent was identified as CPV, based on A-type inclusion and C18L genes-specific PCRs and partial sequencing of the C18L gene, which clearly confirmed that the outbreaks were caused by CPV. Further, phylogenic analysis of partial C18L gene of the isolated CPV and the Vaccinal strain (Jouf-78) of CPV sequence have showed that the isolated CPV clustered together with other reported isolates of CPV on contrast the vaccinal strain clustered with other vaccinai virus.
  B.M. Ahmed , H.A. Hussein and Ahmed A. El-Sanousi
  The unpredictable fast evolution of H3N8 equine Influenza virus and its recent transmission to dogs have raised the importance of a rapid vaccine production strategy. Currently available EIV vaccines are problematic due to their production cost, time and transportation restrictions. DNA Plasmids expressing immunogenic gene (s) are good alternative as it has the advantages of modified living vaccines without the risks of infection and/or reversion to virulence as well as ease of production and handling. In this study, DNA plasmid expressing HA1 (PcDNArHA1) of an H3 Egyptian equine influenza isolate was prepared and its immunogenicity in female wistar rats was evaluated. HA1 protein of PcDNArHA1 was expressed in Vero cell line and detected in the cytoplasm by indirect immunofluorescence. Two doses of ultrapurified PcDNArHA1, 100 μg each were then inoculated 15 day apart in the quadriceps muscle of female wistar rats test group while placebo group inoculated only PBS. Rats were euthanized and the serum collect.
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