Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Articles by H. Zhang
Total Records ( 16 ) for H. Zhang
  Y Zhang , L Bao , H Zhu , B Huang and H. Zhang

Leptospirosis renal disease is one of the common clinical manifestations of leptospirosis, including acute renal failure and tubulointerstitial nephritis. Outer membrane protein A-like protein Loa22 is a lipoprotein from Leptospira interrogans and has been suggested to be a corresponding virulence factor. However, the role of Loa22 in leptospiral nephropathy is not yet understood. In the present study, we constructed a vector and artificially expressed Loa22 in Escherichia coli BL21(DE)pLysS cells. After extensive purification, along with a GST tag protein control, Loa22 protein was used to test the cytotoxicity in cultured rat proximal tubule cells (NRK52E) and examine its effects on the induction of inflammatory responses. Using morphological examination, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazoium hydrixide absorbance, lactate dehydrogenase assays and an analysis of apoptosis via flow cytometry, it was found that Loa22 protein mediates a direct cytotoxic effect on NRK52E cells in a dose-dependent manner. Using real-time PCR, western blotting and immunofluorescence, it was found that Loa22 protein upregulates the expression of toll-like receptor 2 (TLR2), induces nitric oxide synthase and promotes the production of nitric oxide (NO) and monocyte chemoattractant protein-1 (MCP-1) by NRK52E cells. Additionally, using a TLR2 blocking antibody, it was found that enhanced NO and MCP-1 production by NRK52E cells after Loa22 stimulation requires the activation of TLR2. Collectively, our data suggested that Loa22 is a critical virulence factor of L. interrogans and is involved in the leptospiral nephropathy through mediating direct cytotoxicity and enhancing inflammatory responses.

  X.-C. Zhang , L. A. Hunt , W. A. Phillips , G. Horn , J. Edward and H. Zhang
  Computer models must be thoroughly evaluated before being used for decision-making. The objective of this paper is to evaluate the ability of a newly developed wheat grazing model to predict fall–winter forage and winter wheat (Triticum aestivum L.) grain yield as well as daily weight gains of steer (Bos taurus) grazing on wheat pasture in Oklahoma. Experimental data of three independent field studies were used. The first was a variety trial in which fall–winter forage and grain yields were harvested. The second was a planting date experiment in which forage in the fall–winter period and grain yields were harvested. The third was a steer grazing experiment in which standing wheat biomass and steer weight gain were monitored. For the variety trials, the model efficiency (ME), which reflects how well model predictions match measured data (1 means a perfect match), was 0.102 for fall–winter forage prediction and 0.367 for grain yield. For the planting date experiment, the ME was 0.615 for predicting fall–winter forage yields and 0.409 for grain yields when a root downward extension rate of 20 mm d–1 was used. In the steer grazing experiment, the relationship between average daily weight gain and forage allowance was adequately represented by the model. For the total steer weight gains in a wide range of stocking rates and grazing durations, the ME was 0.616. Overall results show that the model, if well calibrated, has the potential to predict fall–winter forage and grain yields as well as mean daily weight gain per steer.
  H. Zhang

We present an effective cohesive discrete crack method in the context of the Reproducing Kernel Particle Method (RKPM) in order to study fracture of concrete structures. The discrete crack approach is based on the visibility method and a simple node splitting scheme. We also present an effective implementation of the visibility method and an iteration free algorithm by including the cohesive force term directly into the stiffness equations. The crack is represented by straight-line segments and the cohesive zone model is employed to model the post-localization behavior of concrete. The method is applied to several examples involving mode I and mixed-mode fracture. These results are compared to experimental data and show good agreement.

  Z. DING , Y. XU , H. ZHANG , S. WANG , W. CHEN and Z. SUN
  An experiment was conducted in the laboratory to investigate the effects of additive ratios of docosahexaenoic acid (DHA) to eicosapentaenoic acid (EPA) on the growth and survival of cobia (Rachycentron canadum) juveniles from August to October 2005. Three hundred and eighty cobia juveniles (56 days of age, body weight 6.9 ± 0.1 g, body length 9.2 ± 0.1 cm) were selected and 20 of them were freely taken for initial sample analysis in the week 0. Additional 360 juveniles were randomly assigned into eight groups with triplicate, total 24 tanks with 15 fish each. Cobia juveniles were reared in glass-steel tanks (200-L volume per tank) using filtered seawater with temperature 26–30.5 °C, salinity 25.4–33.0 g L−1 and pH 7.8–8.0. Cobia juveniles were fed for 8 weeks using seven treatment diets (D-1 to D-7) with the same amount of DHA and EPA (15.0 ± 1.2 g kg−1 of dried diet), but varying ratios of DHA to EPA (0.9, 1.1, 1.3, 1.5, 1.7, 1.9, 2.1, respectively) and a control diet (D-0, DHA + EPA = 8.0 g kg−1 of dried diet, DHA/EPA = 1.3). Five juveniles per tank were randomly taken for sample analysis at the end of weeks 4 and 8, respectively. The highest protein efficiency rate (PER; 1.5 in mean), average body weight (BW; 73.3 g per fish in mean) and the lowest feed conversion ratio (FCR; 1.6 in mean) were obtained in cobia juveniles fed the control diet at the end of week 8. These parameters were significantly different (P < 0.05) among juveniles fed the control and treatment diets; however, no significant difference (P > 0.05) was found among juveniles fed the treatment diets evaluated in this study. It was concluded that the survival and growth of cobia juveniles were not greatly influenced by additive ratios of DHA to EPA in our experimental conditions.
  H. Zhang , T.W. An , J.W. He , Y.Z. Luo and J.L. Han
  Domestic yak plays a critical role in supporting the livelihoods of nomads in the Central Asian Highlands. Tianzhu White yak is a unique breed developed from a very small number of mutant founders and its white hair has a special niche market value. In this study, the genetic polymorphisms in Tyrosinase (TYR) gene, which has been considered as a ‘albino locus’ in cattle, were identified and characterized to search for alleles associated with the white coat colour in yak. A total of 973 yak samples were collected, including 438 animals from five nucleus breeding herds and 365 individuals from four reproductive herds of the Tianzhu White yak. The reference TYR genomic DNA sequence derived from a Hereford bull was used to design all primers for screening and sequencing the exon 2 and the last partial 5’-untranslated region (5’-UTR) of the yak TYR gene. Both PCR-SSCP analysis and DNA sequences for their complete exon 2 in selected samples from the Tianzhu White yak herds and black yak populations identified a single conserved sequence identical to other cattle breeds. There were 14 genotypes and seven alleles defined by nucleotide polymorphisms present in the 215 bp long 5’-UTR of yak TYR gene among all yak samples, of which five alleles were specific to yak while the other two alleles were of a cattle origin. Although, current data suggested no association of these polymorphisms with the yak coat colour variations, they shed light on the potential function of the promoter on regulation of expression of yak TYR gene that is warranted to screen for additional polymorphisms in its extended 5’-UTR and other exons.
  A. Cheikhyoussef , N. Pogori , F.W. Tian , W. Chen and H. Zhang
   The antimicrobial activity of four strains of Bifidobacterium toward Salmonella ssp. during associated cultures growth was investigated in skim milk medium. All strains showed different degrees of antagonistic action toward the indicator strain. The highest degree of inhibition (96%) was obtained with Bifidobacterium infantis and Bifidobacterium longum (92%). The combination effect of yogurt mixed culture with bifidobacterial strains (di-associated culture) toward Salmonella ssp. resulted in an enhancement of the antagonistic action for Bifidobacterium strains as a result of their production of organic acids, in particular lactic acid, which has a strong inhibitory effect against Gram-negative bacteria. The di-associated cultures all resulted in similar pH values but the degree of inhibition were different with B. infantis and B. longum; meaning that organic acids are not the sole inhibitory factors present in these cultures, but it could be another compounds which may contribute in this inhibitory effects. The combination between Bifidobacterium and YMC strains could has a great value in industrial application in resolving some problems in dairy products and pharmaceutical formulas.
  T. D Butters , O. V Aslanidi , S Inada , M. R Boyett , J. C Hancox , M Lei and H. Zhang

Rationale: Familial sick sinus syndrome (SSS) has been linked to loss-of-function mutations of the SCN5A gene, which result in decreased inward Na+ current, INa. However, the functional role of INa in cardiac pacemaking is controversial, and mechanistic links between mutations and sinus node dysfunction in SSS are unclear.

Objective: To determine mechanisms by which the SCN5A mutations impair cardiac pacemaking.

Methods and Results: Action potential (AP) models for rabbit sinoatrial node (SAN) cells were modified to incorporate experimentally reported INa changes induced by 2 groups of SCN5A gene mutations (affecting the activation and inactivation of INa, respectively). The cell models were incorporated into an anatomically detailed 2D model of the intact SAN-atrium. Effects of the mutations and vagal nerve activity on cardiac pacemaking at the single-cell and tissue levels were studied. Multielectrode extracellular potential recordings of activation pattern from intact SAN-atrium preparations were performed to test predictions of the models. At the single-cell level, the mutations slowed down pacemaking rates in peripheral, but not in central SAN cells that control the heart rhythm. However, in tissue simulations, the mutations not only slowed down pacemaking, but also compromised AP conduction across the SAN-atrium, leading to a possible SAN exit block or sinus arrest, the major features of SSS. Simulated vagal nerve activity amplified the bradycardiac effects of the mutations. Two groups of SCN5A mutations showed subtle differences in impairing the ability of the SAN to drive the surrounding atrium, primarily attributable to their differential effects on atrial excitability and conduction safety. Experimental data with tetrodotoxin and carbachol confirmed the simulation outcomes.

Conclusions: Our study substantiates the causative link between SCN5A gene mutations and SSS and illustrates mechanisms by which the mutations impair the driving ability of the SAN.

  X. j Zhou , J. c Lv , D. f Bu , L Yu , Y. r Yang , J Zhao , Z Cui , R Yang , M. h Zhao and H. Zhang

Anti-glomerular basement membrane antibody disease (anti-GBM disease) is a rare disorder characteristic of universally poor outcome. Fc receptors (FcRs) play important roles in anti-GBM disease based on evidence from animal models. Copy number variation (CNV) influences disease susceptibility. The FcRs genes show CNV, and CNV of the FCGR3B gene is associated with glomerulonephritis in systemic lupus erythematosus and anti-neutrophil cytoplasmic antibody-associated small vasculitis. Here, we investigated CNV of three FCGR genes, including two (FCGR3A and FCGR3B) for activating FcRs and one (FCGR2B) for inhibitory FcR by duplex quantitative real-time PCR. Copy numbers were analyzed by Applied Biosystems CopyCaller Software v1.0. We first demonstrated the distribution of CNV of FCGR3A, FCGR3B and no CNV of FCGR2B in Chinese population (including 47 anti-GBM patients and 146 healthy controls). The frequency of CNV of FCGR3A was observed to be significantly higher than matched healthy controls (27.7 versus 12.3%, P = 0.013, odds ratio 1.21–6.10). Considering previous report about gene knock-out animal models and CNV effect of FCGR3A, we thus propose that CNV in members of FCGR family should have different roles in the pathogenesis of human anti-GBM disease.

  Y.J. Li , L. Zhang , L.Q. Shang , H.F. Wang , H. Zou , H. Zhang and D.J. Ji
  Using PCR-SSCP method, the relationship between genetic polymorphisms at three reproduction associated loci and litter size were explored in Chinese Haimen goat. The results showed that intron 1 of Prolactin Receptor (PRLR) gene had two genotypes and three genotypes were also found in intron 2 of the same gene while no polymorphism was detected in FSHR gene. The least square analysis and multiple comparisons of the polymorphisms at PRLR gene loci and litter size indicated that variations of PRLR gene intron 2 correlated extremely significantly with litter size in Haimen goat. These findings demonstrated that PRLR gene could be used as a candidate genetic marker for fecundity in goat.
  X.D. Hu , K. Jia , G.H. Zhang , Z.X. Lin , H.Y. Xia , D.W. Zhang , X.B. Zeng , H.S. Qiao , H. Zhang and S.J. Li
  Several Enzyme-Linked Immunosorbent Assay (ELISA) methods exist to diagnose Bovine Tuberculosis (BTB). In this study, an ELISA method was established with a mixture of esat-6 and cfp-10 as the diagnostic antigens. ELISA tests on 160 bovine serum samples (80 from cattle with BTB; 80 from healthy cattle) demonstrated that the sensitivity was 76.3% (61/80) and the specificity was 97.5% (78/80). A further screening test using ELISA and PPD was conducted with 335 serum samples collected at random from eight dairy herds (5 problematic herds and 3 BTB-negative herds) in Guangdong, China. In the problematic herds, the positive ELISA rate was 7.9% (16/202) for anti-esat-6 and cfp-10 antibodies and 5.4% (11/202) in the PPD. This study demonstrated that ELISA with the use of the esat-6 and cfp-10 mixture of antigens is simple and sensitive and can be used to analyze large numbers of samples for the serodiagnosis of BTB.
  T.X. Li , K. Kuwana , K. Saito , H. Zhang and Z. Chen
  We have conducted experimental and numerical studies on flame synthesis of carbon nanotubes (CNTs) to investigate the effects of three key parameters – selective catalyst, temperature and available carbon sources – on CNT growth. Two different substrates were used to synthesize CNTs: Ni-alloy wire substrates to obtain curved and entangled CNTs and Si-substrates with porous anodic aluminum oxide (AAO) nanotemplates to grow well-aligned, self-assembled and size-controllable CNTs, each using two different types of laminar flames, co-flow and counter-flow methane–air diffusion flames. An appropriate temperature range in the synthesis region is essential for CNTs to grow on the substrates. Possible carbon sources for CNT growth were found to be the major species CO and those intermediate species C2H2, C2H4, C2H6, and methyl radical CH3. The major species H2, CO2 and H2O in the synthesis region are expected to activate the catalyst and help to promote catalyst reaction.
  C Zhao , W Zhuo , B Chen and H. Zhang

For calibration and intercomparison experiments, a thoron chamber with an inner volume of 300 l was designed based on a programmable constant temperature and humidity testing device in this work. The commercial lantern mantles enriched with 232Th were used as the 220Rn source and the mantles were set in 3x3x3 points of lattice style inside the chamber. Experimental studies showed that 220Rn concentrations in the chamber could be easily controlled and adjusted from about 0.5 to 80 kBq m–3 through manual settings of the relative humidity and temperature, and the spatial distribution of 220Rn in the chamber was fairly homogeneous.

  J. L. Schroder , H. Zhang , D. Zhou , N. Basta , W. R. Raun , M. E. Payton and A. Zazulak
  The USEPA Part 503 rule did not directly specify the amount of sludge-borne P that can be applied but allowed application rates based on the recommended N requirement of a crop. Monitoring of long-term application of biosolids is important to protect both soil and water quality. Biosolids and ammonium nitrate were annually applied for 13 yr to winter wheat at six plant available N (PAN) rates: 0, 45, 90, 180, 269, 536 kg N ha–1 yr–1. Biosolids application did not increase soil pH but increased levels of total C, total N, NH4–N, NO3–N, and electrical conductivity (EC). Soil concentrations of Cd, Cu, Pb, Mo, and Zn were greater in biosolid-treated plots but were <24% of their respective cumulative pollutant loading rates. Application of biosolids increased plant micronutrient B, Cu, Fe, and Zn concentrations. The addition of biosolids to the Norge soil (fine-silty, mixed, active, thermic Udic Paleustoll) increased Mehlich 3 plant available P (M3P) and water soluble P (WSP) and these P levels were well correlated with biosolids application rate. Application of biosolids at rates of >=2 times the recommended agronomic rate of 90 kg PAN ha–1 resulted in M3P concentrations that exceeded an environmental threshold of 200 mg kg–1 established by the USDA-NRCS for the land application of manures in Oklahoma non-nutrient limited watersheds. Significant relationships were found between the ammonium oxalate P saturation index (PSIox) and M3P as well as between PSIox and WSP. It appears the repeated long-term application of biosolids above the N agronomic rate should be avoided and application should be based on other criteria such as an agronomic P threshold, an environmental P threshold, or a P site index.
  Y. Wang , Y. He , H. Zhang , J. Schroder , C. Li and D. Zhou
  Phosphate mobilization by organic acids has been shown to be soil type dependent and controlled by the soil`s intrinsic P status. The objective of this study was to evaluate the capacity and mechanisms of three organic acids in the mobilization of phosphate from an upland clay loam Ultisol that had received different long-term fertilizer treatments. Soil samples were incubated at 25 ± 1°C and 40% moisture content for 3 wk with citric, tartaric, or oxalic acid at 1.0 mmol kg–1 of soil. Soil pH, inorganic P fractions, and plant-available P (Olsen P) were analyzed after incubation. The results indicated that Olsen P and the loosely bound P extracted with 1.0 mol L–1 NH4Cl (NH4Cl-P) were significantly increased by the treatment of the three organic acids, and Fe phosphate (Fe-P), occluded phosphate (Oc-P), and Ca phosphate (Ca-P) were mobilized and released to various degrees as well. The order of increased mobilization of P by the organic acids was citric acid > tartaric acid > oxalic acid. These three organic acids have the potential to increase the availability of P in soil but need to be evaluated at the field scale.
  M. Li , G. Ji , F. Feng , W. Song , R. Ling , D. Chen , X. Liu , J. Li , H. Shi , W. Wang and H. Zhang

Objective: We summarized our experience of living-related small bowel transplantation and postoperative management of 3 patients with short gut syndrome.

Methods: Patient #1, an 18-year-old boy, received a 150-cm segment of distal ileum with a vascular pedicle of distal superior mesenteric artery and vein, which was donated by his father. Patient #2, a 15-year-old boy, received a 160-cm graft of distal ileum from his mother. Patient #3, a 17-year-old boy, received a 170-cm graft of distal ileum from his father. The graft artery and vein were anastomosed to the recipient infrarenal aorta and vena cava, respectively, in end-to-side fashion using 7/0 Prolene suture. Intestinal continuity was restored by anastomosis of proximal end of the graft to the recipients' own proximal jejunum, the distal end was left open as a stoma. The recipient distal gut was anastomosed to the distal end of the graft. All 3 recipients were given FK506 (tacrolimus) regularly combined with periodic mycophenolate mofetil. In cases of acute rejection, large doses of steroids were administered to the recipients.

Results: The recipients and donors had fairly unremarkable postoperative courses. So far, patient #1 has survived for 7 years and 6 months with a well-functioning graft and without requirement for total parenteral nutrition (TPN) support. His body weight increased 20 kg and of his life quality has dramatically improved. Patient #2, however, died of acute rejection with fatal sepsis at 5 months after transplantation. Patient #3 has survived for 3 years and 8 months enjoying a normal life. Postoperative recovery of all 3 donors was unremarkable. They were discharged 12 days after surgery without complications.

Conclusion: Outcomes of the implantation using the distal ileum as a graft in living-related small bowel transplantation have been satisfactory for both recipients and donors. It is feasible to anastomose the graft artery and vein to the recipient infrarenal aorta and vena cava. The intestinal continuity can be restored by a 1-stage strategy with minimal risk to the recipient. Appropriate application and adjustment of immune suppressors are crucial for the recipients to experience high-quality lives.
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility