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Articles by H. Soleimanjahi
Total Records ( 1 ) for H. Soleimanjahi
  Jalil F. Mehrabadi , Q. Behzadian Nejad , Sh. Najar Peerayeh , M. Khodabandeh , H. Soleimanjahi and A.M. Hassan
  Uropathogenic Escherichia coli is one of the major agents of urinary tract infection. Since it has intracellular propagation, cellular immune response is so important in this case. Accordingly, a genetic construct for inducing of cellular immune system was designed. At first, chromosomal DNA extracted from E. coli 35218 and fimH gene amplified with this template by PCR. PCR product inserted to pcDNA.1 eukaryotic expression vector and confirmed the recombinant vector by sequencing. The COS7 cell line transfected with a complex of pcDNA/fimH and ExGen 500 poly cationic polymer. Expression of fimH gene in COS7 was confirmed by RT-PCR. Consequently, pcDNA/fimH cassette could express inserted fimH gene in eukaryotic cells and is a valuable DNA candidate cassette for urinary tract infection vaccination. This is the first prompt to designing a DNA vaccine against urinary tract infection that caused by Uropathogenic Escherichia coli.
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