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Articles by H. Ogata
Total Records ( 3 ) for H. Ogata
  A.M. Anwar , K. Hattori , H. Ogata , M. Ashraf and Mandula
  The current research addresses Engineered Cementitious Composites (ECC) as a new alternative for retrofitting damaged concrete beams. Twenty-one plain concrete beams with pre-defined artificial cracks were prepared and repaired using different combinations of ECC alone or together with Carbon Fiber Reinforced Polymers (CFRP). The study showed that replacement of the inferior layer from the bottom of the deteriorated beams with a thin layer of ECC could be able to restore the beam to a condition better than its original state. Moreover, the repair with ECC was found effective in enhancing the member ductility as well. It was also shown that pasting CFRP directly over ECC substrate resulted in shear failure rather than the undesirable interfacial debonding mode of failure that typically occurs in case of concrete substrates.
  A Monier , A Pagarete , C de Vargas , M. J Allen , B Read , J. M Claverie and H. Ogata
 

Interactions between viruses and phytoplankton, the main primary producers in the oceans, affect global biogeochemical cycles and climate. Recent studies are increasingly revealing possible cases of gene transfers between cyanobacteria and phages, which might have played significant roles in the evolution of cyanobacteria/phage systems. However, little has been documented about the occurrence of horizontal gene transfer in eukaryotic phytoplankton/virus systems. Here we report phylogenetic evidence for the transfer of seven genes involved in the sphingolipid biosynthesis pathway between the cosmopolitan eukaryotic microalga Emiliania huxleyi and its large DNA virus EhV. PCR assays indicate that these genes are prevalent in E. huxleyi and EhV strains isolated from different geographic locations. Patterns of protein and gene sequence conservation support that these genes are functional in both E. huxleyi and EhV. This is the first clear case of horizontal gene transfer of multiple functionally linked enzymes in a eukaryotic phytoplankton–virus system. We examine arguments for the possible direction of the gene transfer. The virus-to-host direction suggests the existence of ancient viruses that controlled the complex metabolic pathway in order to infect primitive eukaryotic cells. In contrast, the host-to-virus direction suggests that the serial acquisition of genes involved in the same metabolic pathway might have been a strategy for the ancestor of EhVs to stay ahead of their closest relatives in the great evolutionary race for survival.

  M. Ashraf , A. Goyal , A.M. Anwar , K. Hattori , H. Ogata and S. Guo
  An evaluation was made on the mutual beneficial role of fly ash and ground granulated blast furnace slag in combating adiabatic temperature rise. The experimental program was designed in two stages; the main experiment consisted of two massive concrete specimens with dimensions (50x50x50) cm. In first stage of experiment, an adiabatic rise in temperature of specimens was measured. In second stage, the mechanical properties of massive concrete specimens were measured at the ages of 8, 14, 28, 56 and 91 days. At the age of 91 days, surface core and central cores were extracted from the surface and the central part of massive concrete specimens to determine compressive strength and dynamic modulus of elasticity. In the massive concrete specimen without any additive, the peak temperature noted was 64.5°C at 7th h after casting. While in mineral substituted concrete the maximum adiabatic temperature was 49.6°C at 19th h after casting. Lower rate of temperature rise in mineral substituted concrete has resulted in higher value of ultrasonic pulse velocity and ultimate compressive strength of concrete.
 
 
 
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