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Articles by H. Muhd-Farouk
Total Records ( 3 ) for H. Muhd-Farouk
  M. Ikhwanuddin , A.B. Noor-Hidayati , N.M.A. Aina-Lyana , H. Zulaikha , H. Muhd-Farouk and A.B. Abol-Munafi
  The aim of the study was to develop an appropriate basis for the optimization of in vitro fertilization of Fenneropenaeus merguiensis using three different culture medium including Natural Sea Water (NSW) as control medium, Artificial Sea Water (ASW) and Calcium Free saline (Ca-F saline). The unfertilized mature eggs were collected from the broodstock ovaries during spawning. The non-motile sperm of F. merguiensis activated as natural spawning. In NSW medium, ASW medium and Ca-F saline medium, cortical rods were released and hatching envelope formation took place in which the eggs activation events were reported. The Ca-F saline and ASW solution induced a slow egg activation contradict with the sequence of event for natural spawning of F. merguiensis. Fertilization was successfully obtained in all treatments with 8.67±4.04% in ASW, 19.67±7.38 and 4.33±4.04 in Ca-F saline. Although, the hatching rate were not successfully obtained by ASW and NSW culture medium treatment, hatching yield in Ca-F saline medium was obtained with 3.00±2.65. Overall, these findings will contribute to the development of F. merguiensis breeding technology and further understanding on sperm biology, cryobiology and reproductive biology in shrimp.
  M. Ikhwanuddin , H. Muhd-Farouk , A.J. Memon , W. Wendy and A.B. Abol-Munafi
  The aim of this study was to evaluate how long the fresh sperm maintained at 2°C would be utilized for fishery management. The study was conducted every 2 h to assess the sperm viability of orange mud crab Scylla olivacea. Evaluations were conducted as 3 treatments; T1, T2 and T3. In T1, the live specimens were sacrificed; for T2, only spermatophores were extracted and for T3 spermatophore extraction followed by homogenization to create a sperm suspension. All samples were stored with ice in an insulated box was keep fresh longer at 2°C. The time ‘0’ referred the immediate collection of sperm after the specimen was sacrificed. Spermatophore viability was determined using the sperm suspension by eosin-nigrosin staining method. Sperm viability for the fresh sample at time zero was 97.36±0.53%. Viability of the sperm significantly decreased in the 2nd h in all treatments, T1 was 44.66±0.54 to 4.2 ±0.22% at 16 and 18th h, T2 was 36.56±0.5 to 2.69±0.06% at the 12 and 14th h and T3 was 33.69±1.26 to 6.4±0.29% at 8 and 10th h. In comparison, T1 showed significantly higher than other treatments (p<0.05). Extremely low viability percentages were recorded in T3. This study also proved that the time elapse had significant impact on the percentage of viable sperm count.
  A. Amin-Safwan , H. Muhd-Farouk , M. Nadirah and M. Ikhwanuddin
  Background and Objective: Mud crab from the genus Scylla are considered as one of the most demanded seafood items nowadays as their flesh has high quality, tasty and higher growth rate thus support and boosted expansion in aquaculture sector especially in Malaysia. Present study was designed to focus on the effect of water salinity on the ovarian maturation of orange mud crab, Scylla olivacea based on morphological characteristics. Methodology: Samples were collected from Setiu wetlands, Terengganu, Malaysia from July-September, 2015. Ovarian maturation of S. olivacea was classified into four stages based on previous study which were: Immature (Stage 1), early mature (Stage 2), late mature (Stage 3) and fully mature (Stage 4). Results: Morphologically as the ovary develop the colouration start to change from translucent or whitish in colour and sometimes creamy to pale yellow, follow by light orange and lastly reddish orange. Stage 1 ovary was translucent and whitish in colour, stage 2 ovary was pale yellow in colour, stage 3 was light orange and stage 4 ovary was reddish orange in colour. Gonad Somatic Index (GSI) of S. olivacea remained low at stage 1 and 2 and began to increase started at stage 3. This present study involved three different salinities treatments, which treatment 1 (10 ppt), treatment 2 (20 ppt) and treatment 3 (30 ppt). Treatment 2 produce the highest number of stage 4 ovarian maturation based on colouration and the highest GSI recorded, follow by treatment 1 and lastly treatment 3. Conclusion: This present study proved that salinity does affected the ovarian maturation of S. olivacea in captivity and provides important information regarding the effect of water salinity on ovarian maturation for further studies on reproductive biology of this species.
 
 
 
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