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Articles by H. Motamedi
Total Records ( 7 ) for H. Motamedi
  S.M. Seyyednejad , S. Maleki , N. Mirzaei Damab and H. Motamedi
  The antibacterial activity of parsley (Petroselinum crispum) and Prunus mahaleb seed ethanolic extracts were examined using agar disc diffusion method against eleven bacteria (Bacillus anthracis, Bacillus subtilis, Bacillus pumilus, Staphylococcus aureus, Bacillus licheniformis, Brucella melitensis, Escherichia coli,Salmonella typhi, Proteus mirabilis, Bordetella bronshiseptica, Pseudomonas aeruginosa). These extracts had inhibitory effect at various concentrations (0.1, 0.2, 0.3 and 0.4 g mL-1) against Gram-positive and Gram-negative bacteria. Prunus mahaleb ethanolic extract had antibacterial activity against P. mirabilis, B. anthracis and S. aureus. B. licheniformis was the most sensitive organism to the parsley ethanolic extract. Both of the extracts had inhibitory effect against Br. melitensis, E. coli and B. licheniformis in low concentrations (0.1 and 0.2 g mL-1). Based on the results of this study, both plants could be considered as disinfectants or antiseptics, thus confirming their use in folk medicine.
  H. Motamedi , A. Safary , S. Maleki and S.M. Seyyednejad
  The antibacterial activity of Ziziphus spina-christi leaves ethanolic and methanolic extracts were examined using agar disc diffusion method against eight bacteria (Salmonella typhi, Proteus mirabilis, Shigella dysenteriae, Escherichia coli, Klebsiella pneumoniae, Brucella melitensis, Bordetella bronshiseptica and Pseudomonas aeruginosa). These extracts had inhibitory effect at various concentrations (0.05, 0.1, 0.2, 0.3 and 0.4 g mL-1) against tested bacteria. The ethanolic extract had the highest activity (20 mm) against B. bronchiseptica while the lowest activity (7 mm) was demonstrated by the methanolic extract on K. pneumoniae. Studies on the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the methanolic extract on two selected bacteria showed that the S. dysenteriae had the highest MIC (18 mg mL-1) and MBC (64 mg mL-1) values.
  A. Safary , M. Roayayi Ardakani , A. Abolhasani Suraki , M. Akbarzade Khiavi and H. Motamedi
  Marine biosurfactants produced by some marine microorganisms have been paid more attention, particularly for the bioremediation of the sea polluted by crude oil. The goal of this study was isolation and characterization of biosurfactant producing bacteria from Caspian Sea water. Ten morphologically distinct microbial colonies were isolated and screened for biosurfactant production. From the results, tow strains isolated from Caspian Sea in Iran, which were able to grow on crud oil as sole carbon sources and to produce biosurfactants. Primary screening of biosurfactant-producing colonies was performed using the qualitative drop-collapse test, oil displacement test and hemolytic test. Among tow strains CpA1 completely emulsified crud oil in MSM medium within 48 h of cultivation. This strain exhibited the highest activity for oil displacement test toward crud oil (3.14 cm2) and emulsification activity. In second screening, the surface tension of culture supernatants for CpA1 isolate grown under identical conditions was measured by Captive Drop Cell instrument. This strain reduced the growth medium surface tension below 40 mN m-1 and selected as a best biosurfactant producer in this study.
  A. Safary , H. Motamedi , S. Maleki and S.M. Seyyednejad
  The antibacterial activity of Q. brantii fruits ethanolic and methanolic extracts were examined using agar disc diffusion method against eight bacteria (Salmonella typhi, Proteus mirabilis, Shigella dysenteriae, Escherichia coli, Klebsiella pneumoniae, Brucella melitensis, Bordetella bronchiseptica, Pseudomonas aeruginosa). These extracts had inhibitory effect at various concentrations (0.5, 0.1, 0.2, 0.3 and 0.4 g mL-1) against tested bacteria. The ethanolic extract had the highest activity (30 mm) against Br. melitensis and B. bronchiseptica while the lowest activity (7 mm) was demonstrated by the methanolic extract on E. coli. Studies on the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the methanolic extract on tested microorganisms showed that the highest MIC (20 mg mL-1) and MBC (32 mg mL-1) were demonstrated against Sh. dysenteriae, B. bronchiseptica and P. mirabilis had the highest MIC and MBC values (32 mg mL-1) for the ethanolic extract.
  H. Motamedi , E. Darabpour , M. Gholipour and S.M. Seyyed Nejad
  Plantago ovata (Plantaginaceae) and Oliveria decumbens (Umbliferae) are of important medicinal plants in Iran which have been used in traditional medicine. The aim of present study was to consider antibacterial properties of ethanolic and methanolic extracts of seed husk of Plantago ovata and aerial part of Oliveria decumbens. For this purpose, the 50 to 400 mg mL-1 concentration of these extracts were assayed against six Gram-negative and eight Gram-positive bacteria by disc diffusion method. Synthetic Antibiotic discs were used as control. Staphylococcus epidermidis and Staphylococcus aureus were the most sensitive species to the ethanolic and methanolic extracts of Plantago ovata while Pseudomonas aeruginosa was the most resistant to these extracts. Furthermore, Escherichia coli and Proteus mirabilis have shown resistance to ethanolic extract of this plant. Ethanolic extract of Oliveria decumbens was effective against all of tested bacteria and S. aureus was the most sensitive strain. In the case of the methanolic extract, Salmonella typhi, P. aeruginosa and P. mirabilis were more resistant than the others. The MIC (Minimal Inhibitory Concentration) and MBC (Minimal Bactericidal Concentration) values for Oliveria decumbens extracts against S. aureus were same (20 mg mL-1), whereas against Streptococcus pyogenes were different. Also, MIC for ethanolic extract of Plantago Ovata against S. aureus and Bordetella bronchiseptica were same (20 mg mL-1), whereas for methanolic extract were 20 and 10 mg mL-1, respectively. The MBC for these two bacteria weren’t found (>200 mg mL-1). On the basis of these results it can say that these plants have proper antibacterial effect and can be considered as a new source of antibiotic discovery and development for infectious disease treatment purposes.
  S.M. Seyyednejad and H. Motamedi
  not available
  S. Maleki , S.M. Seyyednejad , N. Mirzaie Damabi and H. Motamedi
  The aim of this study was to examine the antimicrobial activity of the methanolic extract of Torilis leptophylla was tested on eleven bacteria (Bacillus anthracis, Bacillus subtilis, Bacillus pumilus, Staphylococcus aureus, Bacillus licheniformis, Brucella melitensis, Escherichia coli, Salmonella typhi, Proteus mirabilis, Bordetella bronshiseptica and Pseudomonas aeruginosa). Tested extract was effective against all bacteria but not B. subtilis. Consequently, the ethanolic extract had antibacterial activity on some pathogens thus confirming their use in folk medicine.
 
 
 
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