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Articles by H. Liu
Total Records ( 9 ) for H. Liu
  X. -Q. Yang , L. -J. Guo , C. -Y. Zhai , H. Yu , H. Liu and D. Liu
  This study was designed to clone the porcine calpain 7 gene, CAPN7, to characterize its tissue expression, and to investigate its genetic variation. The cDNA sequence that contains a 2442 bp open reading frame encoding 813 amino acids was obtained by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods based on in slico cloning and homologous cloning, and five introns were identified. Quantitative real-time PCR showed that CAPN7 was ubiquitously expressed in all tissues examined, and the mRNA level in muscles was low at 1-7-days-old and high from 90 to 270-days-old. In addition, two single nucleotide polymorphisms in coding sequence were identified and the polymerase chain reaction-restriction fragment length polymorphism methods were established to detect the polymorphisms. At each site, the distribution of three genotypes is significantly different (P<0.01) in Yorkshire, Duroc, Wild boar, and Min pigs.
  H Zhao , Y Wang , Y Wu , X Li , G Yang , X Ma , R Zhao and H. Liu

Hyperlipidemia is regarded as an independent risk factor in the development of ischemic heart disease, and it can increase the myocardial susceptibility to ischemia/reperfusion (I/R) injury. Ischemic postconditioning (Postcon) has been demonstrated to attenuate the myocardial injury induced by I/R in normal conditions. But the effect of ischemic Postcon on hyperlipidemic animals is unknown. Hypoxia inducible factor-1 (HIF-1) has been demonstrated to play a central role in the cardioprotection by preconditioning, which is one of the protective strategies except for Postcon. The aim of this study was to determine whether Postcon could reduce myocardial injury in hyperlipidemic animals and to assess whether HIF-1 was involved in Postcon mechanisms. Male Wistar rats underwent the left anterior descending coronary occlusion for 30 min followed by 180 min of reperfusion with or without Postcon after fed with high fat diet or normal diet for 8 weeks. The detrimental indices induced by the I/R insult included infarct size, plasma creatine kinase activity and caspase-3 activity. Results showed that hyperlipidemia remarkably enhanced the myocardial injury induced by I/R, while Postcon significantly decreased the myocardial injury in both normolipidemic and hyperlipidemic rats. Moreover, both hyperlipidemia and I/R promoted the HIF-1 expression. Most importantly, we have for the first time demonstrated that Postcon further induced a significant increase in HIF-1 protein level not only in normolipidemic but also in hyperlipidemic conditions. Thus, Postcon reduces the myocardial injury induced by I/R in normal and hyperlipidemic animals, and HIF-1 upregulation may involve in the Postcon-mediated cardioprotective mechanisms.

  H. LIU , X. WU , W. ZHAO , M. XUE , L. GUO , Y. YU and Y. ZHENG
  Apparent digestibility coefficients (ADCs) of dry matter (ADCd), crude protein (ADCp), energy (ADCe) and amino acids in selected feedstuffs were determined for juvenile Siberian sturgeon (8.38±0.20g). The tested feedstuffs were fishmeal (FM), meat and bone meal (MBM), poultry by-product meal, hydrolysed feather meal, fermented feather meal solvent-extracted cottonseed meal and soybean meal. ADCs were determined using a reference diet and test diets at 7:3 ratios with 5gkg-1 chromic oxide (Cr2O3) as an inert marker. Fish were reared in a recirculating system and fed to apparent satiation five times daily. Cr2O3 in diets and faeces samples were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) and acid-digestion colorimetry (AC) methods, respectively. The results showed that ICP-AES method was more accurate for Cr2O3 determination than AC method, and the results determined by ICP-AES method were used in this study. ADCd and ADCp of seven tested ingredients were lowest for MBM (59.1 and 84.5%) and highest for FM (79.9 and 94.5%); ADCe of tested ingredients were from 71.8% for SECM to 93.2% for FM. ADCs of amino acid in test ingredients followed similar trend to the ADCp. The ADCs of individual amino acids varied from 61.6% (histidine in MBM) to 98.8% (valine in FM).
  H. Liu , G. Li , W. Zhong , D. Li , F. Liu and W. Sun
  Essential amino acids, particularly those containing the element sulfur, are required by small canids to produce a high quality pelt. The experiments were designed to estimate the effect of a diet supplemented with the sulfur-containing amino acid methionine on the nutrient metabolism and pelt quality of raccoon dogs (Nyctereutes procyonoides). Seventy-five male raccoon dogs with similar body weights were randomly assigned to five dietary groups of 15 each during the winter fur growth period. The diet for the control group contained 24% protein while the diets for groups 1 to 4 contained 20% protein plus 0.15, 0.35, 0.55 and 0.75 g methionine per 100 g dry matter, respectively, for a 60-day period. As a result, the body weights in group 4 were clearly reduced compared to the other groups (p<0.05). Dry matter intake in the control group was significantly higher than for groups 1 and 2 (p<0.05), but was similar to groups 3 and 4. Although, serum methionine level was similar among all groups, group 2 showed significantly higher total protein in serum (p<0.05) than the control group; while serum urea nitrogen in group 2 was lower than that of groups 3 or 4 (p<0.05). The pelt length in the control group and in group 2 was significantly longer than the other groups. The density of guard hair and fiber in the controls and groups 1 and 2 was remarkably higher compared with that in groups 3 and 4 (p<0.05). These results suggest that a certain amount of supplemental methionine may reduce the total protein requirement in the diet without affecting the pelt quality of raccoon dogs.
  Y Gao , Y He , J Ding , K Wu , B Hu , Y Liu , Y Wu , B Guo , Y Shen , D Landi , S Landi , Y Zhou and H. Liu

Hepatocellular carcinoma (HCC) is the fifth most common malignancy caused by environmental and genetic factors. MicroRNAs (miRNAs) are a class of short non-coding RNAs with posttranscriptional regulatory functions. They participate in diverse biological pathways and function as gene regulators. Genetic polymorphisms in 3' untranslated regions (3' UTRs) targeted by miRNAs alter the strength of miRNA binding, with consequences on regulation of target genes thereby affecting the individual's cancer risk. We have previously predicted polymorphisms falling in miRNA-binding regions of cancer genes. We selected an insertion/deletion (Indel) polymorphism (rs3783553) in the 3' UTR of interleukin (IL)-1 (IL1A) for a case–control study in a Chinese population. With samples from 403 HCC patients and 434 healthy control individuals, strong evidence of association was observed for the variant homozygote. This association was validated in a second independent case–control study with 1074 HCC patients and 1239 healthy control individuals (odds ratio = 0.62; 95% confidence interval = 0.49–0.78). We further show that the ‘TTCA’ insertion allele for rs3783553 disrupts a binding site for miR-122 and miR-378, thereby increasing transcription of IL-1 in vitro and in vivo. These findings suggest that functional polymorphism rs3783553 in IL1A could contribute to HCC susceptibility. Considering IL-1 affects not only various phases of the malignant process, such as carcinogenesis, tumor growth and invasiveness, but also patterns of interactions between malignant cells and the host's immune system, our results indicated that IL-1 may be a promising target for immunotherapy, early diagnosis and intervention of HCC.

  L. Zhou , G. Cui , H. Liu , D. Luo and Z. Wu
  The Nth-Powered P-persistent Broadcast protocol (NPPB) is a probabilistic broadcasting scheme, which is designed to alleviate broadcast storms and support effective emergency message disseminations in dense VANETs. In this protocol, a probability p is calculated by the receiver to decide whether it should rebroadcast or not. This method reduces the rebroadcast probabilities more efficiently, which can alleviate the serious competitions and collisions. Comparative analyses have been done in two aspects to compare the new NPPB with traditional schemes. And a saturated traffic jam scenario is established to evaluate the NPPB scheme. Simulations results show that if the value of n is selected appropriately, NPPB can achieve higher performance such as less delay, fewer hops, lower load and higher throughput, at the same time the reliability can be guaranteed.
  C.Y. Wu , G.H. Zhao , Y.Q. Zhao , H. Liu , P.J. Zhang and D.K. Chen
  CD4+CD25+ T cells played a critical role in the establishment and maintenance of peripheral tolerance via adoptive transfer. However, whether one or more molecules in CD4+CD25+ T cells that could independently mediate peripheral tolerance was disputed by worldwide researchers. In the present study, one soluble antigen-specific factor was extracted from splenic lymphocytes lysates of OVA-tolerant mice (named OVA Immune Tolerance Factor, OVA-ITF) with molecular mass <3 ku which could establish OVA-specific immune tolerance in recipient mice via transfer treated and induce the same effect of peripheral tolerance as those of splenic lymphocytes from OVA-tolerant mice. Treated with OVA-ITF to naive BALB/c mice resulted in significant suppression of DTH reaction and T cell proliferation in an antigen-specific manner as well as a significant increase in the percentage of CD4+CD25+ T cells within the CD4+ T cell subset in peripheral blood. Present study showed that OVA-TIF was produced by CD4+CD25+ T cell subset and could induce OVA-specific peripheral tolerance independently in vivo with TGF-β1 as its main suppressive cytokine in recipient mice. These results suggested that OVA-TIF is a novel, low MW factor and totally different from other suppressive components reported previously which have important implications for expanding new potential therapeutic routes of prevention and control of graft rejection, autoimmune and related diseases.
  J. Xu , K. Yin , H. Liu , J.H.W. Lee , D.M. Anderson , A.Y.T. Ho and P.J. Harrison
  Eutrophication impacts may vary spatially and temporally due to different physical processes. Using a 22-year time series data set (1986–2007), a comparison was made of eutrophication impacts between the two harbours with very different hydrodynamic conditions. Victoria Harbour (Victoria) receives sewage effluent and therefore nutrients are abundant. In the highly-flushed Victoria, the highest monthly average Chl a (13 μg L1) occurred during the period of strongest stratification in summer as a result of rainfall, runoff and the input of the nutrient-rich Pearl River estuarine waters, but the high flushing rate restricted nutrient utilization and further accumulation of algal biomass. In other seasons, vertical mixing induced light limitation and horizontal dilution led to low Chl a (< 2 μg L1) and no spring bloom. Few hypoxic events (DO < 2 mg L1) occurred due to re-aeration and limited accumulation at depth due to flushing and vertical mixing. Therefore, Victoria is resilient to nutrient enrichment. In contrast, in the weakly-flushed Tolo Harbour (Tolo), year long stratification, long residence times and weak tidal currents favored algal growth, resulting in a spring diatom bloom and high Chl a (10–30 μg L1) all year and frequent hypoxic events in summer. Hence, Tolo is susceptible to nutrient enrichment and responded to nutrient reduction after sewage diversion in 1997. Sewage diversion from Tolo resulted in a 32–38% decrease in algal biomass in Tolo, but not in Victoria. There has been a significant increase (11–22%) in bottom DO in both harbours. Our findings demonstrate that an understanding of the role of physical processes is critical in order to predict the effectiveness of sewage management strategies in reducing eutrophication impacts.
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