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Articles by H. Kumar
Total Records ( 4 ) for H. Kumar
  S.G. Chethan , S.K. Singh , M. Karikalan , N.S. Kharayat , B.K. Behera , K. Narayanan , H. Kumar and A. Anjaneya
  Infertility or sterility due to uterine pathology is one of the major reasons for culling of buffalo in India. Abattoirs serve as a good source of biological samples in order to study different pathological conditions. Major affections of uterus in buffalo include endometritis, metritis, pyometra, hydrometra, mucometra and certain congenital abnormalities. Mostly diagnosis of such cases is based on gross morphological examination observed in abattoir or sometime on clinical and post-mortem examination. Histological based diagnosis of major affections of uterus in buffalo is less and as such very few reports are available especially on hydrometra and mucometra conditions. The present study reports the morphological and histopathological evaluation of cytological and purulent endometritis, hydrometra and mucometra conditions in riverine buffalo.
  N. Kapoor , A. Arya , M.A. Siddiqui , A. Amir and H. Kumar
  Accelerated ageing test were made in five chickpea varieties viz., three desi (Pusa 256, Pusa 212 and BGD 72) and two kabuli (Pusa 267 and K551) with the objective of studying sequence and relationship of process of deterioration over short periods. All the varieties were investigated to consider whether there are cultivars differences for tolerance to ageing conditions. In the study, effect of accelerated ageing on seed viability, seedling vigour and biochemical process were explored. The overall results showed that seed quality in chickpea deteriorates following accelerated ageing treatment. All the physiological parameter viz., germination percentage, seedling root length, shoot length and consequently the vigour index in all varieties were significantly decreased. An increase in moisture content was observed during ageing. Decrease in seed viability and germination rate, vigour is also correlated with biochemical changes (decreased soluble proteins and sugar content) associated with seed ageing. The study also exhibited differential responses among varieties with respect to ageing. All the test experiments performed concluded that Pusa 256 is the best variety, followed by K 551 among all five varieties and Pusa 212 is highly sensitive variety with respect to accelerated ageing.
  D.P. Saxena , S.K. Shukla , K. Kumar , R. Saxena , S. Saxena , S. Shukla , V. Gupta , R. Stephen , H. Kumar and L. Kumar
  The aim of this study was to test the in vitro antiplasmodial effect of extract and different fractions of Diospyros melanoxylan. Diospyros melanoxylon (Barks) are collected from the lower forest of Uttaranchal, which is identified by the local people. Plant materials are known as source of new antimicrobial agents, as a result search has been to discover new antibacterial drugs of plant origin. Six of eight methanolic extracts were found to have significant activity, in vitro anti-plasmodial effect of the water and ethanol extract of IC50 value is ranging from (IC50 = 116±9.19 and IC50 145±140 μg mL-1) and (IC50 = 35±0.0 and IC50 47±4.24 μg mL-1) of D. melanoxylon against chloroquine resistant and susceptible variety of Plasmodium falciparum. Least phytochemicals was observed in case of petroleum ether. These results, so obtained demonstrate the broad spectrum activity of D. melanoxylon bark extracts which may be useful in treatment of various microbial infections. Extracts of three of the four species of Indian Diospyros tested in this study showed antiplasmodial activities, with the best activity shown by D. melanoxylon. This species especially is worthy of further investigation to determine which of its constituents are responsible for the activity.
  N. Kapoor , M. Tyagi , H. Kumar , A. Arya , M.A. Siddiqui , A. Amir and A.S. Malik
  The aim of the present study was production of cellulase enzyme using wheat straw as substrate from Chateomium sp. under Solid State Fermentation (SSF) condition. The culture parameters, such as duration of incubation, incubation temperature, pH, carbon and nitrogen sources and additives, were optimized for enhancing cellulase yield. The optimal level of each parameter for maximum cellulase production by the fungus was determined. Results showed that cellulase production was highest with wheat straw containing production medium supplemented with sucrose, malt extract and CaCl2 and the incubation temperature, time and pH were 35°C, 3rd day and 6.5, respectively. The importance of cellulase enzyme in industries cannot be over emphasized. The crude enzyme when purified may serve the importance of this enzyme in both refining and deinking of recycled papers.
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