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Articles by H. Kermanshahi
Total Records ( 11 ) for H. Kermanshahi
  R. Majidzadeh Heravi , L. Roozbeh Nasiraii , M. Sankian , H. Kermanshahi and A.R. Varasteh
  To date, lactobacilli are widely used in food industries and new probiotic products; hence these are considered as an attractive target for genetic modifications. This study was conducted to improve electroporation of probiotic lactobacilli which is a necessary prerequisite for genetic manipulations. Four strains of probiotic lactobacilli from different sources were grown in Man Rogosa and Sharp (MRS) broth medium containing glycine, as a cell wall weakening agent or a pulse of glycine for evaluation of glycine effect on electroporation efficiency. After evaluation of various parameters such as washing buffers, washing times, electric field strength, pulse duration and plasmid concentration, a practical electroporation protocol was presented to improve electrotransformation of lactobacilli in comparison with two standard protocols. This new protocol exhibited higher transformation efficiency (transformants/μg plasmids) than standard methods (p<0.05) with no differences between species (Lb. casei, Lb. crispatus, Lb. salivarus and L. rhamnosus). The pulse of glycine had no effect on the number of the transformants in three methods. In contrast to plasmid concentration, plasmid size had no influence on the transformation efficiency. The modified method enabled to transform plasmid into the resistant lactobacilli against transformation. These bacteria have potential for bioengineering research to improve special performance.
  M. Emadi , H. Kermanshahi and E. Maroufyan
  An experiment was conducted to study the effect of Turmeric Rhizome Powder (TRP) on some blood parameters in broiler chickens. A corn-soybean meal based diet containing levels of TRP (0.00, 0.25, 0.50 and 0.75%) in a completely randomized design with 4 treatments and 5 replicates of 10 birds each were fed to 200 day-old Ross male broiler chickens from 0-49 days of age. For measuring blood parameters (albumin, total protein, total cholesterol, HDL and LDL-cholesterol and triglyceride) at 21, 35, and 42 and red blood cells, hemoglobin and hematocrit value at 21 and 42 days of age, serum blood samples from each replicate of treatments were collected. Inclusion of TRP into the diets significantly increased total cholesterol, HDL-cholesterol and haemoglobin and decreased LDL-cholesterol, VLDL-cholesterol and red blood cells at 42 days of age (p<0.05). TRP also significantly decreased blood albumin (p<0.05). Under the condition of this study, it was concluded that TRP might have some positive effects on health status of the broiler chickens.
  A. Hassanabadi and H. Kermanshahi
  An experiment was conducted using 6168 Hyline White hens (79 wk of age) randomly assigned to two dietary treatments. The treatments included high dietary zinc (Zn) and Feed Withdrawal (FW) methods. In the Zn method, hens were fed a layer ration containing 20,000 ppm of zinc as zinc oxide for 10 d and the light was reduced to 12 h/d. At day 11, hens were returned to the control layer ration and received 16 h of light/d. In FW method, feed deprivation was continued for 5 d, water was provided for ad libitum intake and the photo period was reduced to 12 h/d. On day 6, were returned to the control layer ration and received 16 h of light/d. Data were analyzed using student`s T-test procedure of SAS. The hens subjected to Zn and FW methods lost 5 and 20% of their initial body weight by 11 and 6 days of the experiment, respectively. The FW treatment resulted in total cessation of egg production within 7 d and the birds remained out of production until 16 d of the experiment. Hens subjected to Zn treatment ceased egg production by 8 day of the experiment and remained out of production until day 29. The mortality percentage throughout the entire experiment for Zn and FW were 0.057 and 0.032%, respectively which was significantly different. No differences were observed between feed removal treatment and nonfeed removal treatment for postmoult hen day production, egg weight, feed intake, feed efficiency and internal Zn of the eggs laid after Zn treatment. Our results indicated that nonfeed removal method is as effective as feed removal method on postmolt performance, although it caused significantly more mortality.
  H. Kermanshahi , A.R. Hazegh and N. Afzali
  In an in vivo study, Sodium Bentonite (SB) was evaluated for its ability to reduce the deleterious effects of Aflatoxin B1 (AFB1) in broiler chickens. In this study, 288 days old Ross male broiler chickens were used and randomly assigned to 9 treatment groups, with 4 replicates of 8 birds each. Three levels of Aflatoxin B1, AFB1 (0, 500 and 1000 ppb) and 3 levels of SB (0.0, 0.5 and 1.0%) were fed to chickens from 0-42 days of age. Feeding levels of AFB1 alone significantly decreased (p<0.05) feed intake, body weight and body weight gain, while, SB levels alone or in combination with AFB1, had no effect on the mentioned traits. No significant difference was seen for feed conversion ratio during periods of 0-42 days of age. In the diet containing AFB1, relative weight of the carcass, thigh and breast were significantly decreased (p<0.05). Adding AFB1 alone into the diets significantly increased (p<0.05) serum ALT, AST and LDH enzymes and decreased serum GGT enzyme (p<0.05). However, SB alone or in combination with AFB1 had no effect on the above mentioned enzymes. It is suggested that sodium bentonite at the levels of 0.5-1.0% might be used for reducing the adverse effects of aflatoxins in broiler chickens.
  J. Bahram Pour and H. Kermanshahi
  A study was conducted to evaluate the effects of healthy broiler breeder undefined cecal culture and commercial probiotic (PremaLac®) on broiler performance and the serum lipids of broiler chickens from 1-42 days of age. A total of 288 days old Ross male broiler chickens were used in a 3x2 factorial arrangement with three levels of cecal cultures (0.00, 2.50, 5.00 mL L-1 in drinking water) and two levels of PremaLac (0.00, 0.09% in the diet). The cecal cultures and PremaLac were used >7 days of age. Body weight gain was significantly increased in cecal culture-inoculated compared to control broilers at the critical period of 0-21 days of age. A higher body weight gain was also observed in PremaLac-treated compared to control broilers for the whole growing period of 0-42 days. The feed intake and feed conversion ratio decreased in PremaLac-fed compared to control broilers from 0-21 days of age. Cecal cultures and PremaLac did not decrease serum lipid components. More research is needed to clarify the beneficial or adverse effect of cecal cultures as probiotics in poultry.
  H. Hashemipour , H. Kermanshahi and M. Pilevar
  In this experiment sodium bentonite was evaluated for its effect on egg yolk pigmentation and performance when synthetic pigment was added to the diet of laying hens. About 256 Hy-Line W-36 laying hens 35 weeks old were allocated to 8 experimental diets with 4 replicates each. Four levels of sodium bentonite (0.0, 1.0, 2.0 and 3.0%) and 2 levels of synthetic pigment (0.0 and 0.04%) were used for the 12 week experiment with a 4x2 factorial arrangement and a completely randomized design. Pigment was supplied by 20 g of lucantin and 20 g of xanthin that were diluted with 9960 g wheat bran. About 1 week before the experiment, the birds fed dietary treatments for adaptation. The measured records were feed intake, feed conversion ratio, egg production, egg weight, specific gravity, shell percent, shell thickness and yolk color index. The addition of sodium bentonite decreased (p<0.05) the specific gravity and yolk color index compared to the control diet. The addition of pigment significantly increased specific gravity and as expected, increased the yolk color index compared to the control diet.
  M. Mehri , H. Nassiri-Moghaddam , H. Kermanshahi and M. Danesh-Mesgaran
  There is scanty of information on Threonine (Thr) requirements of mixed-sex broiler chickens during the grower period. An experiment was conducted to determine the digestible Thr requirements of straight-run Ross 308 broilers from 15-28 days of age. Basal diet consisting of wheat, triticale and corn gluten meal was formulated to meet or exceed the nutrient requirements except for Thr. Graduation levels of supplemental Thr were added to the basal diet at expense of corn starch generating six treatments to provide a range from 0.46-0.81% of digestible Thr. Both male and female birds were randomized across 48 floor pens (4 replicates and 12 birds per each replicate) in a completely randomized design and each pen was fed one of six amino acid levels from 15-28 days of age. Body weight gain, feed intake, feed conversion, digestible Thr intake and mortality were measured during the experimental period. Body Weight Gain (BWG) and Feed Conversion (FC), Feed Intake (FI) and daily Thr intake responded quadratically to graded levels of digestible Thr (0.46-0.81% of diet). Digestible Thr requirements were estimated using a linear broken-line and quadratic broken-line models. Based on broken-line linear model, digestible Thr requirement for straight-run Ross 308 broilers was determined to be 0.69 and 0.76% for BWG and FC, respectively. However, using broken-line quadratic model, digestible Thr was estimated at 0.79% for BWG.
  M. Mehri , H. Nassiri-Moghaddam , H. Kermanshahi and M. Danesh-Mesgaran
  There is little information on Lysine (Lys) requirements of mixed-sex broiler chickens during the grower period. An experiment was conducted to determine the digestible Lys requirements of straight-run Ross 308 broilers from 15-28 days of age. Basal diet consisting of wheat, triticale and corn gluten meal was formulated to be adequate in all essential amino acids except for Lys. Graduation levels of supplemental Lys-HCl were added to the basal diet at expense of corn starch generating six treatments to provide a range from 0.60-1.20% of digestible Lys. Both male and female birds were randomized across 48 floor pens (4 replicates and 12 birds per each replicate) in a completely randomized design and each pen was fed one of six amino acid levels from 15-28 days of age. Body weight gain, feed intake, feed conversion, digestible Lys intake and mortality were measured during the experimental period. Body Weight Gain (BWG) and Feed Conversion (FC), Feed Intake (FI) and daily Lys intake responded quadratically to graded levels of digestible Lys (0.60-1.20%). Digestible Lys requirements were estimated using a linear broken-line and quadratic broken-line models. Based on broken-line linear model, digestible Lys requirement for straight-run Ross 308 broilers was determined to be 0.95 and 1.08% for BWG and FC, respectively. However, using broken-line quadratic model, digestible Lys was estimated at 1.05% for BWG.
  S.J. Hussaini , H. Nassiri Moghaddam and H. Kermanshahi
  An experiment was conducted to study the influence of different levels of Brewers Spent Grain (BSG) and Enzyme Supplementation (ES) in the diet on the productive performance and digesta viscosity of broilers from 7-42 day of age. Six treatments were arranged factorially with 3 levels of BSG (none, 7.5 and 15%), unsupplemented and supplemented with 0.05% enzyme. Treatments were formulated with the same AMEn and protein. Each treatment was replicated four times (consist of 12 chicks per replicate). During the experimental period daily weight gain, feed conversion and feed intake evaluated. At 21 and 42 days one bird from each replicate killed for measuring digesta viscosity. The results indicated a significant reduction in weight gain, feed intake and increase in feed conversion in broilers by feeding 15% BSG at during the starter period in comparison of control group (p<0.05). ES improved body weight, feed intake and daily weight gain in starter period (p<0.05). Digesta viscosity in broilers decreased with feeding 15% BSG in starter period (p<0.05). It was concluded that broiler performance is improved by ES at early ages. Also, BSG may be used up to 7.5 and 15% of the diets of broilers at starter and grower period, respectively.
  A.H. Piray and H. Kermanshahi
  This experiment was conducted to investigate the effects of Aspergillus meal prebiotic (Fermacto) on production performance, serum lipids and immune responses in broiler chickens. One hundred and forty four day-old Ross male broiler chicks were randomly assigned to three dietary treatments: a basal diet (control), a basal diet with 0.15% Fermacto and basal diet with 0.30% Fermacto. The production parameters, including Feed Intake (FI), Body Weight Gain (BWG) and Feed to Gain Ratio (FCR) were monitored weekly serum lipids, antibody productions and antibody titer against Infectious Bursal Virus Disease (IBVD) were measured at the end of a 6 week trial. At 28 days of age, four birds from each replicate of treatments were injected intrabreast with 0.2 mL of 5% Sheep Red Blood Cell (SRBC). The supplementation of 0.30% Fermacto in broiler diets improved the body weight gain from 21-28 and 35-42 days of age (p<0.05). The BWG was also significantly higher (p<0.01) in Fermacto-fed broilers than that of control treatment at 28-35 days of age, overall growing (21-42 day) and rearing (0-42 day) periods. FI was significantly (p<0.05) higher for 0.15% Fermacto-fed broilers than that of control broilers at 14-21 and 0-21 days of age. FI was significantly greater (p<0.05) in 0.30% Fermacto-fed broilers compared to control broilers at 35-42 days of age, during growing (21-42 day and rearing (0-42 day) periods. From 28-35 days of age, a lower FCR (p<0.05) was observed with 0.15% Fermacto when compared with control group. There were also no significant differences in total cholesterol, high density lipoprotein cholesterol (HDL-cholesterol), low density lipoprotein cholesterol (LDL-cholesterol) and triglycerides among treatments. Dietary Fermacto did not affect antibody titer against IBVD. The serum IgG, IgA and IgM production significantly (p<0.01) decreased in Fermacto-fed broilers as compared to control broilers.
  S. Ghazanfari , H. Kermanshahi , M.R. Nassiry , A. Golian , A.R.H. Moussavi and A. Salehi
  The aim of this study was to investigate the effects of feed restriction and different energy and protein contents of the diet on performance and growth hormone concentration in broiler chicken. Five hundred and seventy six day old Ross male broiler chicks were used in a 2x2x3 factorial arrangements in a completely randomized design experiment. Feeding programs consisted of ad libitum and Skip-a-Day (SAD) feed restriction, two energy levels (3100 and 2800 kcal ME kg-1) and three protein levels (22.3, 19.3 and 16.3% CP). Feed restriction (SAD) was applied during 22-32 d of age. Corn-soybean meal based diets containing vegetable oil were used. Body weight and feed intake were recorded weekly. At 21, 32 and 49 day of age, one bird from four replicate of each treatment was selected randomly to collect blood sample and then carcass, breast and thigh weight were measured. Blood samples assayed for Growth Hormone (GH) concentration by RIA. Feed restriction decreased feed intake and body weight gain (p<0.001) of birds during 22-32 and feed intake during 32- 49 day of age, while body weight gain was not affected during this period. Also, feed restriction decreased Feed Conversion Ratio (FCR) (p<0.01) and body weight gain (p<0.001) during 22-32 day of age. Feed intake (p<0.001) and body weight gain increased in broilers fed on low-energy diets compared with those fed on high-energy diets during different periods. Increasing levels of protein increased feed intake (p<0.001), body weight gain and improved FCR (p<0.001) as compared with least level of protein. Feed restriction decreased carcass percentage (p<0.001) and increased thigh percentage (p<0.01) at 32 day of age. Carcass percentage (P<0.05) and breast percentage increased in broilers fed on low-energy diets compared with those fed on high-energy diets during different periods. The low protein diet decreased carcass percentage (p<0.01), breast percentage (p<0.001) during different periods and thigh percentage (p<0.05) at 21 day of age. The result of this experiment indicated that the lowest protein level had the highest growth hormone concentration at 49 day of age. The low energy diet increased growth hormone concentration (p<0.05) at 21 day of age.
 
 
 
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