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Articles by H. Attar
Total Records ( 3 ) for H. Attar
  F. Seyedzade and H. Attar
  In recent years constraint based methods such as Flux Balance Analysis (FBA) has widely applied for computation of flux distributions in the metabolic networks. In this study, the effects of changes in the intercellular concentration on the Gibbs free energy of the system and subsequently on the model’s fluxes have studied. This method which makes a correlation between the flux directions and metabolite concentrations, has applied to large scale metabolic network, Escherichia coli iAF1260. The biomass and succinate fluxes have selected as objective functions and the multi objective genetic algorithm has used to optimization of the E. coli iAF1260 network. The obtained results revealed that a living system such as E. coli and its mutants are not stable from thermodynamic point of view before reaching to the cellular threshold concentrations. Also the behavior of the mutants of microorganism involving their return to the wild type phenotypes could be justified.
  A. Mellati , H. Attar and M.F. Farahani
  Living Saccharomyces cerevisiae (S.c.) was microencapsulated with a Sol-Gel method using Tetra Methyl Ortho Silicate (TMOS) as a precursor so that it saved its bioactivity. A W/O emulsification technique was used. A vegetable oil was the organic phase for microencapsulation. Bioactivity of immobilized yeast in microcapsules was investigated, measuring the amount of CO2 released from culture as a result of fermentation. Production of CO2 by encapsulated yeast increased about 150% more than free yeast. Repetition of the tests has proved that microcapsules were saved their bioactivity during a month. On the other hand, particle size was decreased from 175 to 110 μm by increasing the mixer rate from 600 to 1200 rpm during gelation and smaller particles showed more bioactivity up to 30%.
  H. Attar and S. Afshar
  In recent years the use of microbial sensors has widely applied for monitoring environmental contamination. In this study, we focus on the effects of biocides such as heavy metals, pesticides and herbicides on bioluminescent bacterium, vibrio fischeri strain DSM 7744 which is used as stable bioindicators. This method makes a correlation between the light of Vibrio fischeri and the concentration of biocides. However, the basic part of this research depends on how to optimize the best condition for maximum bioluminescence. Optimized conditions of Vibrio fischeri were stirring at 120 rpm at a incubation temperature within the range of 23 to 26°C after 24 to 48 h when solid cultures were reserved at 18°C. In this case we use the whole bacteria, Vibrio fischeri which is one of interesting bioluminescence bacteria, coupled with luminometer. In our procedure the LOD for two pesticides, Malathion and Diazinon, and two heavy metals, Mercury and Selenium is about 1ppb.
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