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Articles by H. Abtahi
Total Records ( 4 ) for H. Abtahi
  H. Abtahi , A. Ghazavi , M. Karimi , S. Mollaghasemi and G. Mosayebi
  The aim of this study was to test the antimicrobial activity of the methanolic extract of bitter apricot seeds. Bitter apricot seeds used in folk medicine in the treatment of skin diseases and parasitic diseases. It been traditionally used to treat parasitic infections and skin diseases. Water and methanol extracts of bitter apricot seeds were screened against some bacterial strains. Seeds were extracted by percolation method. Aliquots of the extracts at variable concentrations were then incubated with different bacterial strains and the antimicrobial activities of the water and methanolic extracts from bitter apricot seeds were determined by MIC. Three antibiotics were used as reference compounds for antibacterial activities. Bitter apricot seeds extract inhibited significantly the growth of the tested bacterial strains. Among the bacterial strains tested, Staphylococcus aureus was most susceptibility. The highest antibacterial was exhibited by water extract. Results from these findings suggest that this bitter apricot seeds extract may be used as natural antibacterial for treatment of some of diseases, especially local skin diseases.
  H. Abtahi , A.H. Salmanian , S. Rafati , G.B. Nejad , M. Saffari , A. Ghazavi and G. Mosayebi
  This study was evaluated the ability of DNA vaccine encoding L7/L12 protein of Brucella sp. to induce cellular and humoral immune responses in BALB/c mice and the profile of cytokines and IgG sub classes were determined. Intra muscular vaccination of mice using L7/L12 gene. Three vaccinations at 3 week intervals were performed. Cytokines and IgG subclasses were analyzed 3 week after the last DNA vaccination. Splenic lymphocytes from L7/L12pCDNA3-vaccinated mice produced high levels of IFNγ (3100 pg mL-1) and low levels of IL-5 (300 pg mL-1), 3 weeks post-vaccination. The L7/L12pCDNA3 immunizations elicited high IgG2a isotype response in mice immunized. This antigen also induced IgG1 titers which were slightly lower than the IgG2a titers. Immunological analysis shows the appropriate immune response in BALB/c mice model after vaccination with L7/L12 gene. The high level of IFNγ and low level of IL-5 in combination with high IgG2a/ IgG1 ratio show the activation of Th1 cell response. The lower bacterial cfu from vaccinated mice in comparison with control groups show the efficiency of L7/L12 DNA vaccination in mice model.
  A. Ghazavi , G. Mosayebi , H. Salehi and H. Abtahi
  In this study, effect of ethanol extract of Saffron (Crocus sativus L.) in the treatment of Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 mice was evaluated. EAE was induced by immunization of 8 week old mice with MOG35-55 with complete Freunds adjuvant. Therapy with saffron was started on day the immunization. Total Antioxidant Capacity (TAC) was assessed by Ferric Reducing-Antioxidant Power (FRAP) method. Nitric oxide (NO) production was also estimated by Griess reaction. For histological analysis, mice brain was harvested and sections were stained with Hematoxylin-Eosin. After daily oral dosage the saffron significantly reduced the clinical symptoms in C57BL/6 mice with EAE. Also, treated mice displayed a delayed disease onset compared with control mice. TAC production was significantly elevated in saffron treated mice. Effect of saffron on serum NO production was not significant. Typical spinal cord leukocyte infiltration was observed in control mice compared with saffron treated mice. These results suggest for the first time that saffron is effective in the prevention of symptomatic EAE by inhibition of oxidative stress and leukocyte infiltration to CNS and may be potentially useful for the treatment of Multiple Sclerosis (MS).
  S. Mahmoudi , H. Abtahi , A. Bahador , G. Mosayebi and A.H. Salmanian
  Streptokinase (SK) is a potent plasminogen activator with widespread clinical use as a thrombolytic agent. In this study, we produce high level expression of recombinant streptokinase in E. coli by expression vector pET32a. Genomic DNA of streptokinase gene (SKC) was extracted, then amplified by polymerase chain reaction (PCR) method and sub-cloned to prokaryotic expression vector pET32a. Escherichia coli BL21 (DE3) pLysS were transformed with pET32a-skc and gene expression was induced by IPTG. The expressed protein was purified by affinity chromatography by Ni-NTA resin. High concentration of the recombinant protein obtained from the single-step purification by affinity-chromatography (Ni-NTA). The yield of recombinant streptokinase was nearly 470 mg L-1 of initial culture. Our data showed that production of recombinant streptokinase improved by pET32a in Escherichia coli.
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