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Articles by H. Tayefi-Nasrabadi
Total Records ( 4 ) for H. Tayefi-Nasrabadi
  Z. Rajabi , H. Tayefi-Nasrabadi , A.B. Syofi and H. Janmohammadi
  The use of vaccination in poultry to control AI viruses, especially Mildly Pathogenic Avian Influenza (MPAI) viruses has been increasing in recent years. The amount of antigen mass and hemagglutination activity of Oil-Emulsion (OE) vaccines is important for the control of Avian Influenza (AI) viruses. With extraction of antigen mass of an AI OE-vaccine and determination of Hemagglutination (HA) titers and total protein; it is possible to predict the quality and protective efficacy of the vaccine. In this study, antigen mass was recovered from three commercially available AI OE-vaccines by aqueous partition method, then, the amount of extracted total protein and recovered HA activity were compared with the serologic responses caused in chickens by each vaccine. The results showed that the aqueous partition method retrieves antigen mass from the inactivated AI subgroup H9N2 OE-vaccines and also indicated that in three examined vaccines there is a good correlation among recovered HA activity, extracted total protein and antibody response in each vaccine. This study also showed that Determination of both recovered HA activity and extracted total protein let us know more about the efficacy of an OE-vaccine.
  R. Asadpour , H. Tayefi-Nasrabadi , G.A. Moghadam and K. Nofouzi
  This study was conducted to determine the levels and relationship between Lacto Peroxidase (LP) activity and somatic cell count (SCC) for diagnosis subclinical mastitis in early lactation of dairy cows. Foremilk samples were obtained from quarters of 80 cows (August 2007 up to February 2008). Any cows had not evidence of clinical mastitis at time of sampling. The SCC ranged from 5.24105 cells/mL in the first parity to 5.5105 in the third parity with a mean value of 5.45105. The mean LP activity in first, second and third parity were found 6.49, 4.63 U and 5.5 U mL 1, respectively. No significant correlation (r = 0, p>0.05) was found between number of SCC and LP values in early lactation. Since, the measurement of LP activity of milk may not be used as a predictor subclinical mastitis in the early lactation period of dairy cows.
  H. Tayefi-Nasrabadi , Gh. Moghaddam , Z. Rajabi and Gh. Dehghan
  For clarifying some possible mechanism of cadmium toxicity, the effect of increasing amounts of Cd2+ ion on peroxidase activity was investigated in vitro in serum of cow. The H2O2-mediated oxidation of o-dianisidine was used to assess the peroxidase activity. Results show that after preincubation of serum with 0.25-100 mM Cd2+ concentration for 5 min, peroxidase activity was inhibited compared to the control and decreased rapidly with increasing metal concentrations. The enzyme was completely inhibited after 5 min preincubation in 100 mM Cd2+. When the preincubation of serum and Cd2+ was prolonged to 0.5, 1 and 24 h, the enzymatic activity decreased more rapidly with increasing metal concentration and the enzyme was completely inhibited at lower metal concentrations (at 50 mM Cd2+ after 30 min preincubation, at 30 mM Cd2+ after 60 min preincubation and at 5 mM Cd2+ after 24 h). By considering of long biological half-life of cadmium in body of animals, it suggested that the damage caused by exposure to heavy metals is often not only dose-dependent, but also time-dependent. Even though detoxifying enzymes may not show any effect after brief exposure to low concentrations of heavy metals, prolonged incubation will affect the enzymatic activity, leading eventually to complete inactivation.
  R. Asadpour , H. Tayefi-Nasrabadi , G.A. Moghadam and K. Nofouzi
  Association between values for the Somatic Cell Count (SCC) and intramammary infection (IMI) were studied in 80 dairy cows from dairy herd in Iran during the first 5-15 days post calving. Samples were cultured for bacterial presence and were tested for SCC. Intramammary infection was defined as the presence of one or two bacterial species in milk samples taken within 5-15 day postcalving. Prevalence of IMI was large; 65% of milk samples were infected. Approximately 30% of the cows classified as infected with Coagulase-negative Staphylococci (CNS) and S. aureus had the pathogen identified on 5-15 days post calving. Streptococcus agalactia accounted for 10% of the IMI. Remaining IMI were by other pathogeneses among which Escherichia coli, Proteus sp., Klebsiella sp. Arcanobacter pyogenes. All milk samples from dairy cows in early lactation had SCC ranged from 5.24105 cells mL 1 in the first parity to 5.5105 in the third parity with a mean value of 5.45105. No significant differences were observed (p>0.05) in SCC values between parities. Also, no significant differences were found (p>0.05) between SCC values and infections. Thus, these testing strategies may not be ideal for making decisions about individual animals, such as identifying individual cow with S. aureus for segregated milking.
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