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Articles by H. Hajarian
Total Records ( 2 ) for H. Hajarian
  M. Dashtizad , H. Wahid , O. Abas Mazni , Y. Rosnina , M. Daliri and H. Hajarian
  Development of efficient culture system to support embryonic development would be valuable when percentage of produced embryos reaching to the blastocyst stage is important. However, the rate of bovine embryo production in vitro is still lower than expected. Present study was performed to investigate the effect of ghrelin on nuclear maturation and subsequent bovine embryo development in vitro. Cumulus-oocyte-complexes were collected from slaughterhouse ovaries and randomly allocated in each treatment groups. Five different concentrations of ghrelin (0, 5, 50, 500 and 1000 ng mL-1) were added to the in vitro maturation medium (Hepes-buffered medium 199+fetal calf serum+gonadotrophins+insulin+antibiotics). The proportion of oocytes developed to metaphase II stage was significantly increased at 5 and 50 ng mL-1 ghrelin (86.32±3.38 and 89.77±2.92%, respectively). The result also indicated that adding high concentration of ghrelin adversely affect (p<0.05) the nuclear maturation rates of bovine oocytes. However, the subsequent embryo development was not significantly affected by addition of ghrelin to the IVM medium. This study showed that inclusion of 5-50 ng mL-1 ghrelin in maturation medium may have beneficial effects on nuclear maturation of bovine oocytes in vitro.
  M.M. Moeini , A.A. Moghaddam and H. Hajarian
  A trial was conducted to evaluate the effects of FGA (Fluorogestone acetate) and CIDR (Controlled internal drug release) on the induction of estrus and pregnancy and fecundity rates of the Sanjabi and Lori sheep. A total of 360 Sanjabi and Lori sheep were randomly grouped into two treatments with intravaginal devices inserted for 13 days: Group FGA (40 mg FGA, n = 180) and Group CIDR (n = 180). All ewes received an i.m. injection of 400 IU eCG (equine chorionic gonadotrophin) at devices removal. Estrous was assessed by exposing all ewes to vasectomized rams at 12 h intervals. Cervical artificial insemination was performed 12 h after estrus onset. The overall estrus response was 72.5%. The source of progestin did not influence the efficiency of estrus response but a significant difference (p<0.05) was found between the breed groups (Lori: 88.6%, Sanjabi: 58.3%). Among the sheep that received either CIDR or FGA, estrus response was significantly (p<0.05) higher in the Lori (CIDR: 82.2%, FGA: 91.1%) than in the Sanjabi (CIDR: 64.4%, FGA: 52.2%) breed. The lambing and fecundity rates for all groups were 60.2% and 1.2±0.03, respectively. No significant differences in term of the lambing and fecundity rates were recorded between CIDR and FGA groups and among Lori and Sanjabi breed. The results of this study indicate the source of progestin or sheep breed did not influence the pregnancy and fecundity rates. The sheep breed influences the estrous response rate while the source of progestin did not affect the estrous response.
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