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Articles by H. Galbraith
Total Records ( 2 ) for H. Galbraith
  A.M. Tahmasbi , H. Galbraith and J.R. Scaife
  Responses of biotin in the pre-ruminant and immediately post-ruminant kids were studied in an experiment using 6Angora and 8 Scottish Cashmere male castrated kids. They were allocated into two equal groups and given a good quality biotin supplemented milk replacer up to 42 days of ages and then they allocated to continue to receive the biotin adequate diet or a diet without supplemented biotin and containing the biotin-binding protein avidin in dried egg white up to 84 days of ages. Thereafter all kids were continued to receive a progressively reducing quantities of the milk diet and in addition a commercial creep feed and chopped hay ad libitum. After weaning at 18 weeks of age, they received chopped hay (0.75) and concentrate (0.25) ad libitum to the end of the study at 24 weeks. Responses in live weight gain, dry matter intake, fibre characteristics, hair loss by combing, Plasma biotin and level of glucose, urea and PCV were measured through the experimental periods. The result indicated that at the end of the pre-ruminant period, biotin deficiency had a significant effect on reducing live weight gain (p< 0.05), feed intake (p< 0.01) and increasing combed hair loss (p< 0.001). Total hair loss of mohair due to biotin deficiency was significantly (p< 0.01) higher than cashmere. Following the introduction to solid feed there were no differences in animal performance due to diet. Total clean fibre yield by Angora goat in all periods was significantly (p< 0.001) higher that for the Cashmere goats. While biotin supplemented milk replacer diet increased plasma biotin concentration this did not result in any significant increase in glucose, urea and PCV in all periods of study. However, during solid feeding Cashmere kids had a significantly higher live weight gain (p< 0.05) and dry matter intake (p< 0.01) than Angora kids. Difference between two genotypes was noted during soild feeding in superior values of feed intake(0.01), live weight gain (p< 0.01), glucose concentration (p< 0.001) and PCV% (p< 0.001) in Chashmer than Angora goats.
  A.M. Tahmasbi , H. Galbraith and J.R. Scaife
  An experiment was conducted to develop an in vitro technique to study the role of biotin in controlling growth and viability of sheep wool follicles. Small samples of skin wee taken from mid-rib are of three Suffolk wether lambs and after discarding epidermal layer, anagen secondary wool follicles were isolated from the dermal layer and maintained in William`s E Medium supplemented with 0.0,0.25 or 0.5 mg L 1 biotin. The results showed significantly increase in vitro wool shaft elongation up to 144 h culture in media. There was no significant differences between rate of fibre elongation of viable cultured follicles. Biotin supplementation significantly increased the proportion of follicles remaining viable after 72 h with the order of response of 0.5>0.25>0.0 mg L 1 supplement. ATP concentration in follicles maintained in the medium containing 0.50 mg L 1 biotin were significantly (p< 0.01) higher than those maintained in medium containing 0.0 or 0.25 mg L 1. Hair follicles maintained in the presence of 0.5 or 1.0 mg L 1 biotin exhibited a significantly (p< 0.01) higher incorporation of [U-14C] Leucine into protein than in follicles in unsupplemented media. DNA concentration per follicle was not affected by biotin concentration. Visual examination of the bulb of hair follicles confirmed the occurrence of mitotic bodies in the matrix of follicles and indicated the presence of viable cells in growing follicles after 144 h of incubation. An examination of cell proliferation sites, using Brdu, in the hair follicles maintained in the presence of 0.0 or 0.50 mg L 1 biotin showed that majority of mitotic activity was concentrated in the bulb adjacent to dermal papilla and outer root sheath in fresh and growing follicles, up to 72 h incubation in different treatments.
 
 
 
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