Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by H Tateno
Total Records ( 2 ) for H Tateno
  K Mikami , D Yamaguchi , H Tateno , D Hu , S. Y Qin , N Kawasaki , M Yamada , N Matsumoto , J Hirabayashi , Y Ito and K. Yamamoto
 

Misfolded glycoproteins are translocated from the endoplasmic reticulum (ER) into the cytoplasm for proteasome-mediated degradation. OS-9 protein is thought to participate in ER-associated glycoprotein degradation (ERAD). The recombinant biotinylated mannose 6-phosphate receptor homology (MRH) domain of human OS-9 (OS-9MRH) together with six kinds of mutated OS-9MRH were prepared and mixed with R-phycoerythrin (PE)-labeled streptavidin to form tetramers (OS-9MRH-SA). The PE-labeled OS-9MRH-SA bound to HeLaS3 cells in a metal ion-independent manner through amino acid residues homologous to those participating in sugar binding of the cation-dependent mannose 6-phosphate receptor, and this binding was greatly increased by swainsonine, deoxymannojirimycin, or kifunensine treatment. N-Acetylglucosaminyltransferase I-deficient Lec1 cells, but not Lec2 or Lec8 cells, were also strongly bound by the tetramer. OS-9MRH-SA binding to the cells was strongly inhibited by Man1,6(Man1,3)Man1,6(Man1,3)Man and Man1,6Man. To further determine the specificity of native ligands for OS-9MRH, frontal affinity chromatography was performed using a wide variety of 92 different oligosaccharides. We found that several N-glycans containing terminal 1,6-linked mannose in the Man1,6(Man1,3)Man1,6(Man1,3)Man structure were good ligands for OS-9MRH, having Ka values of approximately 104 M–1 and that trimming of either an 1,6-linked mannose from the C-arm or an 1,3-linked mannose from the B-arm abrogated binding to OS-9MRH. An immunoprecipitation experiment demonstrated that the 1-antitrypsin variant nullHong Kong, but not wild-type 1-antitrypsin, selectively interacted with OS-9 in the cells in a sugar-dependent manner. These results suggest that trimming of the outermost 1,2-linked mannose on the C-arm is a critical process for misfolded proteins to enter ERAD.

  K Mitsunaga , J Harada Itadani , T Shikanai , H Tateno , Y Ikehara , J Hirabayashi , H Narimatsu and T. Angata
 

Human C21orf63 is a type-1 transmembrane protein of hitherto unknown function, with two repeats of putative ‘galactose-binding lectin domains'. By using glycan microarray analysis and other assays, we found that human C21orf63 interacts with heparin and to a lesser extent with heparan sulphate. The C-terminal galactose-binding lectin domain of C21orf63 is necessary for heparin binding. The inability of other human proteins with galactose-binding lectin domains to interact with heparin suggests that heparin binding is a unique property of C21orf63. Results of real-time polymerase chain reaction and tissue immunostaining imply that C21orf63 is expressed on epithelia of various human tissues.

 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility