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Articles by Gustavo Graciano Fonseca
Total Records ( 5 ) for Gustavo Graciano Fonseca
  Gustavo Graciano Fonseca and Regina Vasconcellos Antonio
  Polyhydroxyalkanoates (PHAs) are thermoplastic, biodegradable polyesters, synthesized by some bacteria from renewable carbon sources. However, its application is limited by the high cost of production. To reduce these costs, recombinant strains that use diverse carbon sources have been developed. In this study, it was studied PHAs production by recombinant Escherichia coli (DH10B and JM10), harboring the structural genes of the polyhydroxyalkanoate synthases of Pseudomonas aeruginosa, using hydrolyzed corn starch and soybean oil as substrate, cheese whey as supplement and acrylic acid as fatty acids β-oxidation inhibitor. Their effect on the cell mass and the PHA content had been evaluated through an experimental design 24. The best results had been obtained with DH10B strain: Dry cell weight of 1.02 g L-1 and 23% of PHA (9 mol% 3-hydroxybutyrate, 4.5 mol% 3-hydroxyoctanoate, 30.7 mol% 3-hydroxydecanoate and 55.8 mol% 3-hydroxydodecanoate), in mineral media containing 5% of hydrolyzed corn starch, 5% de cheese whey and 5% of soybean oil, beyond 1 mM of acrylic acid.
  Gustavo Graciano Fonseca and Regina Vasconcellos Antonio
  Polyhydroxyalkanoates (PHAs) are thermoplastic, biodegradable polyesters, synthesized for some bacteria from renewable carbon sources. However, its application is limited by the high cost of production. To reduce these costs, recombinant strains that use diverse carbon sources have been developed. In this study, it was studied PHAs production by recombinant Escherichia coli (DH10B and JM10), harboring the structural genes of the polyhydroxyalkanoate synthases of Ralstonia eutropha and Pseudomonas aeruginosa, using hydrolyzed corn starch and soybean oil as substrate, cheese whey as supplement and acrylic acid as fatty acids β-oxidation inhibitor. The effect of these on the cell mass and the PHA content had been evaluated through an experimental design 24. The best results had been obtained with DH10B strain, with a dry cell weight of 0.92 g L-1 and 5.9% of PHA (0.5 mol% 3-hydroxybutyrate, 44.9 mol% 3-hydroxyhexanoate and 54.6 mol% 3-hydroxyoctanoate), in mineral media containing 5% of soybean oil, beyond 1 mmol of acrylic acid.
  Gustavo Graciano Fonseca
  The hog meat is the most produced and consumed in the entire world. Parallel, due to hog feces and to the residues begotten by cold stores, pollution has been a major problem, faced by both developed and under development countries, especially, under intensive production. Governments, industries and researchers have gathered in order to minimize the problem of pollution generated from this activity. The southern region of Brazil is the main producer of hogs, in addition to such position we find the reality of the water’s quality in the major producer cities. The high consume of water for each processed hog and the effect of the creation of these, with the generation of feces and the elimination of compounds with high ammonia concentration and the consequent result on the atmosphere and waters, does not suit with the current tendencies for environmental and social management. The result of such production may contribute to a loss of life quality. In a general way, big and small producers are searching for an effective strategy for the minimization of pollution, maintaining the market. The majority of strategies converge for the utilization of the potential from the pollutant itself, valuation of the hog, minimizing residues during the process, consequently reducing the environmental impact. The present study features production of hog’s meat processing under the concepts of clean technology, doing a presentation of the involved steps, suggesting procedures for a minimization of residues, valuation of raw materials and generation of new products.
  Gustavo Graciano Fonseca and Regina Vasconcellos Antonio
  Polyhydroxyalkanoates (PHAs) are biodegradable, thermoplastic polyesters produced from renewable carbon sources by a number of bacteria. However, their application is limited by high production costs. One of the strategies aimed to reduce their costs is the development of recombinant strains able to utilize different carbon sources. The optimal conditions for 3-hydroxybutyrate (P[3HB]) production by the recombinant Escherichia coli strain JM101, harboring Ralstonia eutropha PHA biosynthesis genes, in a defined medium containing hydrolyzed corn starch and soybean oil as carbon sources and cheese whey as a supplement had been established earlier. In order to optimize PHA production, the present study investigated the influence of other variables, such as isopropyl-1-thio-β-D-galactopyranoside (IPTG) and acrylic acid addition, temperature, inoculum size and cultivation time, on dry cell weight (DCW) and PHA accumulation through a 25 factorial design. Statistical models suggested that quite good PHA production performances would be obtained in E. coli JM101 cultures grown in a medium lacking IPTG and acrylic acid with an inoculum size of 5% (v/v) and performed at 37°C for 96 h. Theoretically, about 3.5 g L-1 DCW and 75% PHA could be accumulated. Acrylic acid had a negative effect on DCW at its high level (1 mmol) and was unable to promote incorporation of medium-chain-length units into PHA polymers as expected. Remarkably, IPTG, a very expensive inducer of PHA synthesis gene expression had no influence on PHA production.
  Gustavo Graciano Fonseca , Paulo Sergio da Silva Porto and Luiz Antonio de Almeida Pinto
  Salami is a product which preparation time varies notably in function of some parameters, especially its size. This study carried out in a company to verify in industrial scale the possibility of the reduction of the days of process. Drying curves were done for salami with 60 mm diameter and 240 and 260 mm lengths, corresponding to masses of 776 and 852 g, respectively. In the current conditions, the process time was of 38 days. It was concluded that the maintenance of the 7 days of pre-ripening, a reduction in the period of the first ripening from 13 to 7 days and in the second ripening from 18 to 17, totaling 31 days, reached the desired humidity, occurring no alterations in the final product characteristics. Through non-linear regression analysis it was verified that the obtained parameters of the drying curves, had presented excellent adjustment of correlation (R2 > 99%).
 
 
 
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