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Articles by Gustavo Graciano Fonseca
Total Records ( 2 ) for Gustavo Graciano Fonseca
  Gustavo Graciano Fonseca and Regina Vasconcellos Antonio
  Polyhydroxyalkanoates (PHAs) are thermoplastic, biodegradable polyesters synthesized by some bacteria from renewable carbon sources. However, their application is limited by high production costs. To reduce them, recombinant strains that use diverse carbon sources have been developed. In this work, PHA production by recombinant Escherichia coli (JM101), harboring the structural gene for medium-chain-length polyhydroxyalkanoate synthesis from Pseudomonas aeruginosa, was investigated using several vegetable oils (cotton, rice, canola, dendê (palm), sunflower, corn, olive and soybean) and cheese whey as a supplement. The highest PHA yield obtained was 11.64% PHA (20.3 mol% hydroxyhexanoate, 9.9 mol% hydroxyoctanoate, .0 mol% hydroxyodecanoate and 45.8 mol% hydroxydodecanoate) in a mineral medium containing 1% (v v-1) dendê (palm) oil and 5% (v v-1) cheese whey.
  Gustavo Graciano Fonseca and Regina Vasconcellos Antonio
  Polyhydroxyalcanoates (PHAs) are biodegradable polyesters synthesized by numerous prokaryotic organisms from renewable carbon sources. In this study, the stability of three plasmids (pBHR68, pBHR71 and pBHR77), containing the PHA biosynthesis genes from Ralstonia eutropha and Pseudomonas aeruginosa, was investigated in recombinant E. coli JM101 and DH10B cultures performed at 37°C for 96 h. After 72 h of cultivation, cell growth was resumed due to ampicillin depletion, which allowed the recovery of plasmid-free cells. All plasmids showed almost 100% stability for the first 24 h and up to 30-35 generations. The most stable culture, E. coli strain JM101 harboring the plasmid pBHR68, achieved 50% after 110 generations of growth, while, a similar reduction was observed in the other cultures within 60-80 generations. The best performance of the JM101 strain cultures concerning plasmid stability can be attributed to its best adaptability to the environmental conditions and stability of plasmid-host system.
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