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Articles by Gulam Rusul
Total Records ( 7 ) for Gulam Rusul
  H.L. Foo , T.C. Loh , P.W. Lai , Y. Z. Lim , C.N. Kufli and Gulam Rusul
  The effects of addition of different levels of Lactococcus lactis RW18 (RW18) metabolites in drinking water on growth performance, total cholesterol and faecal LAB, Enterobacteriaceae and pH in rats were investigated. The metabolites were prepared by inoculated and incubated the bacteria culture into MRS broth anaerobically. A total of 30 rats were used in this study. The rats were treated on control (without metabolite), 35% RW18 metabolites (v/v, 35% RW18) and 70% RW18 metabolites (v/v, 70% RW18) in drinking water. The initial and final body weights, growth rate, feed intake and feed conversion ratio were not significantly different among the treatment groups. The water consumption for RW18 rats was significantly lower than the control rats. Plasma total cholesterol levels were not significantly among the treatment groups. Faecal LAB count was only found to be significant at the end of experiment. RW18 rats had significant higher faecal LAB counts than the control rats. Reduction of faecal Enterobacteriaceae counts was found after one week of experiment. Faecal pH of rats treated with RW18 was not significantly reduced as compared with the control rats. The results suggest that addition of metabolites in drinking water may reduce and increase the faecal Enterobacteriaceae and LAB counts, respectively.
  Yousr Abdul Hadi , Zaiton Hassan , Gulam Rusul and Son Radu
  A total of 57 Listeria species strains (19 isolates of Listeria monocytogenes and 38 isolates of Listeria innocua ) isolated from different location in the state of Selangor (Seri Serdang, Seri Kembangan, Kajang and Bangi) were characterized by using RAPD-PCR fingerprinting technique. Primer GEN15002 and GEN15009 were chosen whereby it produced reproducible and typeable results in L. monocytogenes (19) and L. innocua (38) isolates examined with the bands sizes ranging from 0.25 to 3.0 kilobase pairs. From the current study, it can be concluded that the RAPD profiles revealed a wide variability and high level of DNA sequence diversity within Listeria species strains tested. RAPD showed a very discriminatory power since several RAPD profiles were obtained among L. monocytogenes and L. innocua isolates and even slight difference between isolates can be detected.
  Ooi Wai Ling , Son Radu , Gulam Rusul , Mohamed Ismail Abdul Karim , Endang Purwati and Samuel Lihan
  A total of 30 strains of Escherichia coli O157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus (ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint among the 30 E. coli O157:H7 strains. Nearly every isolates had a unique fingerprint and that there were no bands that were highly conserved among the isolates. This study suggests that there is considerable genetic heterogeneity among the E. coli O157:H7 strains by ERIC PCR, and that this has application in screening strains from clinical or food samples to detect a virulent strain with a known fingerprint, and to trace its dissemination.
  Yuherman , Son Radu , Gulam Rusul , Lum Keang Yeang , Ooi Wai Ling and Jamal Khair
  DNA fingerprinting by PCR amplification of enterobacterial repetitive intergenic consensus (ERIC) and pulsed-field gel electrophoresis (PFGE) were used to compare environmental and clinical isolates of Vibrio cholerae O1 and non-O1. All the V. cholerae O1 and non-O1 isolates were typable using ERIC PCR. Though PFGE generated banding patterns to discriminate the isolates into twelve fingerprints, eight isolates were untypable by PFGE due to consistent degradation of the bacterial DNA. Based on the dendrogram generated from ERIC-PCR method, three of the clinical isolates (C1, C2 and C3) were closely related to environmental isolates (E6 or E10). The results indicate that ERIC-PCR is a very discriminative and efficient method for studying genetic diversity of V. cholerae isolates.
  Endang Purwati , Zaiton Hassan , Gulam Rusul , Raha Abdul Rahim and Son Radu
  The objective of present work was to optimize the procedures of FDA and USDA for the isolation of Listeria species in imported frozen beef samples marketed in Malaysia. The modifications consisted of direct analysis or storage of samples at 4°C for 24 h prior to analysis, and enrichment at 30°C or 35°C for 24, 48 and 168 h. For both FDA and USDA modified methods, storage at 4°C for 24 h and pre-enrichment at 24 and 48 h were the most efficient. However, the modified FDA with storage at 4°C for 24 h and pre-enrichment for 24 h (30°C and 35°C) and 48 h (30°C and 35°C) yielded more Listeria species. The rates of isolation were markedly affected with prolonged pre-enrichment incubation up to 168 h. The overall conclusion was that the modified USDA isolation method is beneficial when a limited range of the clinically important Listeria species is sought, whilst the modified FDA is needed to estimate the prevalence of Listeria species in the samples examined.
  Son Radu , Rozila Alias , Gulam Rusul , Samuel Lihan and Ooi Wai Ling
  A total of 70 isolates of Escherichia coli O157:H7 isolated from beef samples were examined with respect to plasmid profiles and random amplified polymorphic DNA (RAPD) patterns. All isolates carried the 90 kb pO157 plasmid alone or in combination with other smaller plasmids. Using Gen1-50-02 (5’-CAATGCGTCT-3’), Gen1-50-09 (5’-AGAGGCGATG-3’) and Gen1-50-10 (5’-CCATTTACGC-3’) as primers, respectively, we obtained DNA polymorphisms which allowed us to discriminate the E. coli O157:H7 isolates into one, six and five RAPD patterns; providing bands ranging in size from 0.25 to 4.0 kb. Our results demonstrate that both plasmid profiling and RAPD-PCR fingerprinting methods are suitable tools for a fast and reliable molecular typing of E. coli O157:H7. The RAPD-PCR method is more sensitive with respect to the individualization of isolates and that RAPD-PCR assay could be a valuable technique for epidemiological studies.
  H.L. Foo , T.C. Loh , P.W. Lai , Y.Z. Lim , C.N. Kufli and Gulam Rusul
  The objectives of this experiment were to study the effect of adding different levels of Lactobacillus plantarum I-UL4 (UL4) metabolite in drinking water on the growth performance, plasma cholesterol concentration, faecal LAB and Enterobacteriaceae counts and pH in postweaning rats. A total of 15 female and 15 male post weaning rats were randomly assigned to three groups of drinking water: 100% drinking water, 65% drinking water + 35% UL4 metabolite (35% UL4) and 30% drinking water + 70% UL4 metabolite (70% UL4) for a period of four weeks. The bacteriocin activity for UL4 was 266.67 AU/ml. Daily feed intake, water intake and weekly live weight were measured. Blood plasma from each rat for total cholesterol analysis was obtained at the end of treatment. Faecal samples were taken directly from rectum from each rat and cultured for LAB and Enterobacteriaceae a day before commencement of the experiment, and then on days 7, 14, 21, and 28 after feeding the metabolite. The growth performance of control rats was better than the UL4 groups. The 35 and 70% UL4 rats had a lower total plasma cholesterol concentration compared to the control group. The UL4 groups had significantly lower Enterobacteriaceae counts on week 3 and significantly (p<0.05) higher LAB counts after 2 weeks of experiment. The 35% UL4 rats had lower faecal pH than other treatment groups. In conclusion, the results showed that the addition of UL4 metabolite in the drinking water reduced the growth rate of rats, especially those treated with 70% UL4, decreased the total cholesterol concentration, increased the faecal LAB counts, reduced Enterobacteriaceae counts and faecal pH.
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