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Articles by Guillermo Tellez
Total Records ( 4 ) for Guillermo Tellez
  Gabriela V. G- Verduzco , Guillermo Tellez , Michael H. Kogut , Armando Isibasi and Vianney Ortiz- Navarrete
  Investigations in our laboratories have indicated that an increased resistance to Salmonella enteritidis SE organ infectivity in chickens was conferred by the immunoprophylactic administration of SE-immune lymphokines (SE-ILK). Resistance has been associated with an enhanced heterophilic accumulation within 4 h of ILK injection. Further studies demonstrated that CxCLi2 is produced locally by the host in response to both the SE infection and the SE-ILK suggesting that IL-8 is a major chemotactic factor produced by the host, which aids in mediating the SE-ILK induced recruitment of heterophils to the site of SE invasion. In the present study, two bio-assays confirmed the presence of IFN and IL-2 functions in SE-ILK that may also participate in the establishment of the reported protection against Salmonella infections. The IL-2 and IFN effects detected in this work suggest that bird’s immune response develops similarly to that of mammals, with some features corresponding to the species.
  Emanuel Cejas , Silvina Pinto , Florencia Prosdocimo , Mariano Batalle , Hebe Barrios , Guillermo Tellez and Mauricio De Franceschi
  The effects of a commercially available Polyphenolic Vegetable Extract (PVE) from Schinopsis lorentzii (Bioquina®) for the reduction of coccidiosis in broiler chicks was evaluated. Day-of-hatch male broiler chicks were obtained from a local hatchery and randomly assigned to three experimental groups consisting of 8 chickens per group with four replicates per treatment: Group 1, PVE 10% - challenged; Group 2, untreated - challenged and Group 3, -untreated, non-challenged. Heated brooder batteries were used for housing and chicks were allowed ad libitum access to unmedicated broiler rations and water for the duration of the experiment. On day 21 post-hatch, all birds in groups 1 and 2 were challenged by oral gavage with 3 mL of a mixed suspension of freshly sporulated oocysts of Eimeria tenella (50,000 oocyst/mL), E. maxima (50,000 oocyst/mL) and E. avervulina (50,000 oocyst/mL). Group 3 was sham inoculated with 3 mL of PBS. Body weight gain was obtained seven days after coccidia challenge. Oocyst sheeding was performed at 5, 7 and 10 days PI. Intestinal macroscopic lesions and histological morphometric analysis was performed 7 days PI. No significant differences were observed in terms of total mortality or lesion scores. Supplementation of PVE significantly increased body weight gain, crypt:villi ratio and decreased oocyst excretion (p<0.05), suggesting that PVE may have an impact against a mixed subclinical Eimeria challenge. Further studies on the potential value of this product as a therapeutic or prophylactic anticoccidial agent are currently being evaluated.
  Claudia E. Gutierrez-Fuentes , Luis A. Zuniga-Orozco , Jose Luis Vicente , Xochitl Hernandez-Velasco , Anita Menconi , Vivek Ayamchirakkunnel Kuttappan , Gopala Kallapura , Juan David Latorre , Sherry Layton , Billy Marshall Hargis and Guillermo Tellez
  Probiotics are live microorganisms which, in adequate dose, will increase the beneficial microbial population in gut. A commercial lactic acid bacteria-based probiotic FloraMax-B11® (FM) has shown to have beneficial effect in reducing microbial colonization in broilers. The present study was intended to evaluate the effect of FM on growth performance, bone qualities and morphometric analysis of broiler chickens. In experiment 1, broiler chickens were divided into control or FM treated chickens. Treated chickens received 5 doses of FM. At the end of 30 days, body weight, was recorded and all chickens were humanely killed. Tibias and ileum content were collected. A significant (p<0.05) increase in body weight was observed in the group that received the probiotic treatment when compared with control non treated group. The improved performance was associated with a significant (p<0.05) reduction of energy and protein digested content of the distal ileum as well as bone parameters. Experiment 2 consisted of two independent trials. In each trial, 400 day-of-hatch, broiler chickens randomly assigned to probiotic or control non treated chickens. At days 1, 12, 23, 34 and 45 days of age, treated chickens received the probiotic in the drinking water. In both trials, a significant (p<0.05) improvement in body weight, feed conversion and morphometric changes in gut and tibia were observed in the group that received FM. Estimation of the cost benefit suggested a 1:24 ratio by using FM. The results of this study suggest that the increase in performance and bone parameters in neonatal chickens treated with FM probiotic may be related with improved morphometric changes in the mucosa of duodenum which are also related with improved digestibility.
  Mohammad H. Shahir , Omid Afsarian , Saeed Ghasemi and Guillermo Tellez
  An experiment was performed to evaluate the effects of dietary supplementation of probiotic (Biosof®) and prebiotic (Active-Mos®) on broiler performance, organ weights, blood parameters and antibody titers against Influenza and Newcastle. Three hundred twelve 7-d-old male broiler chicks were randomly assigned to one of 3 dietary treatments for 6 weeks. The dietary treatments were: (1) control diet (without additive), (2) control diet plus probiotic (0.1% of Biosof®)/ton of feed), (3) control diet plus prebiotic (0.1% of Active-MOS/ton of feed). Overall body weight and feed conversion ratio were significantly (p<0.05) improved by dietary inclusion of the probiotic and the prebiotic compared with the control diet. The relative weights of duodenum, jejunum and ileum were greater (p<0.05) for probiotic-fed birds than the control group, however, duodenum relative weight in the prebiotic group was also significantly greater than control group. The serum concentration of cholesterol was lower in the probiotic fed group than prebiotic and control groups. The serum concentrations of total protein, albumin, globulin and albumin/globulin ratio were not affected by dietary treatments. The number of heterophils, monocytes, lymphocyte, eosinophils were not affected by dietary treatments. However, the heterophils to lymphocyte ratio was lower in the probiotic group than prebiotic and control groups. Serum antibody titers against Newcastle was higher in probiotic treatments compared with prebiotic and control groups, but no significant changes were observed in the antibody titers against Influenza. The results of this study suggest that the probiotic and prebiotic can be used as an alternative to antibiotic growth promoters in broiler diets and can improve the immune response to some vaccines.
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