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Articles by Guangping Gao
Total Records ( 4 ) for Guangping Gao
  Ping Rui , Zengjun Ma , Xiangzhai Zhang , Peiguo Li , Guangping Gao , Zongze Yang and Jinhui Zhang
  This study examined hematology and serum chemistry status of adult American minks (Mustla vison), living in the Changli fur farms of Hebei province, Eastern China to provide important baseline data for clinical diagnosis and breeding in the corresponding animal species. The 41 values were compared between males (n = 10) and female (n = 10) minks. The results showed that gender influenced (p<0.05) serum biochemistry values. Male minks had higher (p<0.05) Creatin Kinase (CK) and Alanine aminotrotransferase (ALT) and lower (p<0.05) Amylase (AM) than females. However, the hematology values were not different by genders.
  Guangping Gao , Guisheng Gao , Qiumei Shi , Yanying Zhang , Donglin Zhang and Hong Yan Ding
  To explore the pathogenesis of causeing diarrhea in mink by E. coli, researchers used PCR to detect the pathogenicity island genes LEE (Ler, eaeA) and HPI (irp, fyuA) of three E. coli whose isolation and identification of serotypes are O38, O78 and O29. Researchers designed four pairs of specific primers according to its gene sequence. The genomic DNA as the template for amplification. Results of the three E. coli are that irp2 gene and fyuA gene of HPI were success fully amplified but the LEE (Ler, eaeA) genes were not amplified. Sequence of HPI (irp2, fyuA) showed that the homology about irp2 of the three E. coli is between 98.5 and 99.3%. The homology with reference E. coli’s irp2 gene is between 98.5 and 99.3%.The homology about fyuA of the three E. coli is between 96.7 and 97.4%. The homology with reference E. coli’s fyuA gene is between 95.8 and 99.4%.
  Qiumei Shi , Yanying Zhang , QiuYue Wang , Guisheng Gao , Guangping Gao , Hai Fang , Donglin Zhang , Cuizhen Chen and Xiaomei Lv
  To explore the pathogenic mechanism of Salmonella, serotype was identified and enterotoxin gene stn was detected for 47 strains suspected Salmonella in the Eastern part of Hebei Province. According to the bacterial culture characteristic, physicochemical properties and analysis results of K-3401 semi automatic bacteria identification instrument and identification for another time by Chengdu Institute of Biological Products, enterotoxin gene stn was detected by PCR. The 17 strains of Salmonella gallinarum, 14 strains of Salmonella typhimurium, 4 strains of Salmonella pullorum 2 strains of Salmonella paratyphi A, 2 strains of Salmonella group BO, 5 strains of Salmonella bovismorbificans, 3 strains of Salmonella enteritidis were detected from 47 strains of chicken source of Salmonella. The 45 strains of stn gene were amplified successfully in all 47 strains. The carrying rate was 95.7%. Homology of stn gene of test strains of Salmonella was between 94 and 100%. Evolutionary tree display that different serotypes of Salmonella enterotoxin stn gene divided into 5 groups. There was no homology with other bacterium. Chicken source of Salmonella contained 7 kinds of serotypes in the eastern part of Hebei Province. Among them, Salmonella gallinarum was 36.2% (17/47), Salmonella typhimurium which was advantages serotypes was 29.8% (14/47). The carrying rate of enterotoxin gene stn which had relation with bacterial pathogenicity was 95.7% (45/47).
  Yanying Zhang , Qiumei Shi , Shuqin Cheng , Guisheng Gao , Hai Fang , Guangping Gao , Yuqin Liu , Cuizhen Chen and Qiang Wang
  A total of 54 samples including duodenum, small intestine contents, lymphonodi mesenterici and diarrhea feces were collected from pigs died of diarrhea in Hebei Province of China in 2009~2011. These samples were examined for the presence of E. coli and serotype identification. High pathogenicity island was detected from E. coli isolates. The isolation and identification of O serotype of E. coli were conducted by common method, fyuA and irp2 genes were detected using PCR. The 54 E. coli strains referred to ten serotypes, O38 was the dominant serotype whose proportion was 51.5% (17/33). The positive rate of fyuA gene was 24.4% (11/45), irp2 was 42.2% (19/45), irp2 and fruA was 13.3% (6/45).
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