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Articles by Gregory T. Pharr
Total Records ( 2 ) for Gregory T. Pharr
  Balazs Felfoldi , Hui Wang , Nikhil Nuthalapati , Robert L. Taylor, Jr. , Jeffrey D. Evans , Scott L. Branton and Gregory T. Pharr

Background and Objective: A previous transcriptomics analysis identified the expression of a pleiotropic cytokine, termed leukocyte cell-derived chemotaxin 2 (LECT2) in the bursa of Fabricius of the chick embryo. However, a role for the LECT2 gene product in the bursal microenvironment is unknown at present. The goal for this research project was to validate the expression of the LECT2 gene at the mRNA and protein level in the chick embryo bursa. Materials and Methods: The LECT2 gene transcript levels were determined by quantitative PCR with RNA derived from embryonic B-cells isolated at two different periods of bursal development. Whole protein extracts from the embryonic bursa were evaluated by mass spectrometry. Results: Expression of the LECT2 gene is significantly higher in B-cells from the early stage of bursal development. Mass spectrometry analysis revealed 9 different peptides from the LECT2 protein in the same embryonic period. Conclusion: The LECT2 gene is expressed at both the mRNA and protein level in the early period of bursa development. We postulate that LECT2 may contribute to B-cell migration into microenvironments established by non-lymphoid cells.

  Nikhil Nuthalapati , Tyler A. Burks , Paul B. Siegel , Robert L. Taylor, Jr and Gregory T. Pharr

Background and Objective: The chicken lines selected for high (HAS line) and low (LAS) antibody responses to antigenic challenge represent a valuable model for identifying genes controlling the development of humoral immunity. In this study, we evaluated the expression of receptor tyrosine kinase (RTK) and non-receptor tyrosine kinase (nRTK) genes in the developing bursa from both chicken lines. These genes have a critical role in cell survival, migration and differentiation. In addition, we examined bursas from embryos exposed to testosterone, to detect changes in the expression of these genes when normal bursa development was inhibited. Materials and Methods: cDNA was synthesized from whole bursa RNA and PCR amplified with degenerate primers. The nucleotide sequence of the PCR products was analyzed with the NCBI database. Results: A total of 172 cDNAs from the HAS and 183 cDNAs from the LAS lines were sequenced. The most abundant nRTK and RTK cDNAs were from the Fyn-related kinase (FRK) and Ephrin A1 receptor (EphA1) genes and the expression levels differed significantly between the HAS and LAS bursas. Embryonic exposure to testosterone increased the number of cDNAs from the FRK gene. Conclusion: The significant differences in transcription of the FRK and EphA1 genes due to genetic selection may result in differences in bursal development between the HAS and LAS lines. Testosterone treatment significantly modulated FRK expression, which suggests a role for this gene in the differentiation and organization of the epithelium in the developing bursa.

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