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Articles by Gholamreza Zarrini
Total Records ( 6 ) for Gholamreza Zarrini
  Younes Ghasemi , Alireza Ebrahiminezhad , Sara Rasoul-Amini , Gholamreza Zarrini , Mohammad Bagher Ghoshoon , Mohammad Javad Raee , Mohammad Hossein Morowvat , Farshid Kafilzadeh and Aboozar Kazemi
  Purified L-asparaginase II from Escherichia coli has been supplied and employed in the acute leukemia and other malignant neoplasms chemotherapy. L-asparaginase II gene (ansB) in E. coli is under regulation and certain conditions is needed for expression of this gene. In this investigation ,the various concentrations of modified M9 medium ingredients and various carbon source were tested to optimize the medium for expression and identification of L-asparaginase in E. coli. Finally a semi-quantitative plate assay for L-asparaginase producing Escherichia coli is reported.
  Seyed Mehdi Razavi , Saber Zahri , Hossein Nazemiyeh , Gholamreza Zarrini , Sariyeh Mohammadi and Mohammad-Amin Abolghassemi-Fakhri
  A known furanocoumarin, 8-geranyloxy psoralen, was isolated from n-hexane extract of Prangos uloptera roots by the TLC method. Its structure was determined by comparison of the spectral data with the literature. Cytotoxic effects of the isolated compound were determined by MTT and Tripan blue assays. The antioxidant and antimicrobial potential of the compound were evaluated by DPPH assay and agar dilution method, respectively. The MTT assay results showed that 8-geranyloxy psoralen reduced cell viability of Hela and Mc-Coy cell lines with IC50 values of 0.792 and 0.835 mM, respectively. Based on the Tripan blue asay, the compound has cytotoxic effects, with an IC50 value of 1.26 mM for Mc-Coy cell line. The compound exhibited weak antioxidant potential, with an RC50 value of 0.262 mg mL-1 and high antimicrobial effects against Staphylococcus epidermidis and Candida kefyr.
  Gholamreza Zarrini , Zargham Sepehrizadeh , Mojtaba Tabatabaei Yazdi , Qorbane Behzadiyan Nejad and Zuhair Muhammad Hassan
  Brucella abortus ribosomal L7/L12 protein is a 12 kDa protein encoded by rplL. This gene was amplified by PCR from B. abortus S19 and cloned in pET16b expression vector. The construct was transformed in Escherichia coli BL21 (DE3) PlysS. The expression was performed by addition 1 mM IPTG (isopropyl-beta-D-thiogalacto- pyranoside) in the medium with OD = 0.6 and incubation continued for 1, 2, 4 and 8 h at 37°C. The highest production (23 mg L-1) level was achieved after 4 h incubation of induction. The recombinant protein was purified by Ni-NTA column and characterized by SDS-PAGE.
  Seyed Mehdi Razavi , Gholamreza Zarrini and Ghader Molavi
  Mentha longifolia (L.) Huds. is a perennial rhizomatous herb distributed from Southern Africa, Europe, the Mediterranean region and eastwards into Asia. Mentha species are also generally known under name of Puneh in Iran where they have been used for centuries as tonics, carminative, digestive, stomachic, antispasmodic and anti-inflammatory agent in folk medicine. In the present work, we study antimicrobial and cytotoxic activity of the plant extracts. The antibacterial and antifungal activity of the plant extracts were evaluated using disk diffusion method. We evaluated cytotoxicity of M. longifolia with MTT assay, as well as. Present finding revealed that the methanol extract of the plant leaves were active against all tested bacteria and fungi. The highest inhibitory effect was observed against Erwinia carotovora, a common plant pathogen bacteria, with MIC value of 128 μg mL-1 and inhibition zone of 41 mm. The extract also exhibited high antibacterial activity with MIC value rang of 192-512 μg mL-1 and inhibition zone of 34-40 mm against S. aureus, Enterococcus faecalis, Streptococcus agalactiae and Escherichia coli. The methanol extract of the plant displayed modest to strong antifungal activity against Candida kefyr, Candida albicans, Sclerotinia sclerotiorum and Aspergillus niger with MIC value range of 576-800 μg mL-1 and inhibition zone of 26-33 mm. Our finding showed that M. longifolia methanol extract has cytotoxic activity. The extract reduced the viability of McCoy cells with RC50 value of 1.92 mg mL-1. It was be concluded that M. longifolia extract cab be used as an antiseptic agent and may be also a good candidate to construction of a new plant biopesticide.
  Zahra Moradpour , Maryam Torshabi , Mohammad Ali Faramarzi , Mojtaba Tabatabaei Yazdi , Younes Ghasemi , Hoda Jahandar , Nadia Zolfaghary and Gholamreza Zarrini
  The ability of Nostoc ellipsosporum PTCC 1659, a cyanobacterium strain, for biotransformation of androst-4-en-3,17-dione (AD) was studied. Fermentation was performed in BG-11 medium supplemented with 0.05% AD at 25°C for seven days incubation. The single metabolite obtained was purified using chromatographically methods and characterized as testosterone on the basis of its spectroscopic features. Bioreaction characteristic observed was 17-carbonyl reduction. Time course study showed the accumulation of the product from the third day of the fermentation and reached to the maximum in the seventh day. Production of testosterone was not affected by aeration. Continuously illumination or 16 h light/8 h dark has no effect on the transformation yield. Optimum concentration of the substrate, which gave maximum bioconversion efficiency, was 0.5 mg mL-1 in one batch. Biotransformation was completely inhibited in a concentration above 2.0 mg mL-1.
  Zahra Moradpour , Maryam Torshabi , Mohammad Ali Faramarzi , Mojtaba Tabatabaei Yazdi , Younes Ghasemi , Hoda Jahandar , Nadia Zolfaghary and Gholamreza Zarrini
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