Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Articles by Gholamreza Sepehri
Total Records ( 8 ) for Gholamreza Sepehri
  Gholamreza Sepehri , Nooshin Talebizadeh , Ali Mirzazadeh , Touraj- Reza Mir-shekari and Ehsan Sepehri
  Problem Statement: The contamination rates of Health Care Worker's (HCW) mobile phones and resistance to commonly used antimicrobials were evaluated in three teaching hospitals in Kerman, Iran. So, we examined 150 randomly selected HCWs in three teaching hospitals in Kerman, Iran, 2007. For each HCW a sterile swab moistened with sterile water was rotated over the surface of both sides of his/her phone, a second swab for the sampling of the dominant hand. Both swabs were cultured by the routine methodology in use at laboratory. Plates were incubated aerobically at 37°C for 48 h. Approach: All samples were examined for the antimicrobial activity for commonly used antimicrobials using disc diffusion method. Results: A total of 48 (32.0%, CI95 24.6-40.1%) mobile phones and 59 (39.3%, CI95 31.5-47.6%) of dominant hands had bacterial contamination and Staphylococcus epidermidis was the most commonly cultured organisms from all sites. The resistance rates to commonly used antimicrobials in isolated bacteria from phones and dominant hand varied from 6.7% for cephalothin to 25% for amoxicillin, respectively. Conclusions/Recommendations: The kind of isolated microorganisms and their susceptibility to commonly used antimicrobials from dominant hands were almost similar with those from phones (p<0.05). In conclusion, the results indicated that the rate of bacterial contamination of the HCW's phone is just below 50%, accompanying with a resistance rate to the common used antibiotics in one fourth of all the cases. Therefore, mobile phones could be an important source of nosocomial infections and the spread of bacterial resistance bacteria in medical healthcare settings.

  Farzaneh Esmaeili , Gholamreza Sepehri , Ghollam-Reza Moshtaghi-Kashanian , Mohammad Khaksari , Najme Salari and Ehsan Sepehri
  Problem statement: Artemisia Persia (AP) used for its therapeutic effects in folk medicine whiles some species of Artemisia shown a hypotensive effect. To determine the cardiovascular effect of AP, we tested the pharmacological responses of aqueous and methanolic extracts of AP on blood pressure (BP) and Heart Rate (HR) of normotensive and ephedrine induced hypertensive rats using a tail-cuff computerized registering device.
Hypertension was produced by single dose of ephedrine (40 mg kg-1 IM-1) to raise BP 20-30 mmHg. Animals were fed with different concentrations of each extracts (300, 400 and 500 mg kg-1) by gavage method. BP and HR were recorded before and during 40 min following aqueous and methanolic extracts administrations in 5 min intervals.
The most effective concentration of both extracts to reduced systolic BP of normotensive rats was 400 mg kg-1 after 20 min consumption (p<0.005), while none of administrated doses affected diastolic BP or HR. The efficacy of extracts was tested in hypertensive rats and the results were compared with the effect of enalapril (30 mg kg-1).
Oral consumption of AP extracts after 20 min reduced systolic BP in normotensive and hypertensive rats,while the aqueous extract of AP reduced the BP of hypertensive rats more effectively than enalapril. Hence precaution should be taken when this herb is consumed as a remedy.

  Gholamreza Sepehri , Hamid Zeinali Nejad , Ehsan Sepehri and Saeideh Razban
  Problem statement: Intra-abdominal infections are associated with significant morbidity and mortality. The resulting infection is typically polymicrobial and comprised of both aerobic and anaerobic microbes, which need systemic antimicrobial therapy. Since the bacteriology and antimicrobial susceptibility of postoperative intra abdominal infections were not determined in Iran, so this study was performed to evaluate the antimicrobial susceptibility patterns among aerobic bacteria isolated from post-operative intra-abdominal selected samples in 2 teaching hospitals in Kerman, Iran. Approach: the peritoneal sample of 174 patients which undergone abdominal surgery were cultured by routine microbiological methods for aerobic microorganisms. Antimicrobial susceptibility testing was performed to 4 commonly used antibacterials (Ampicillin, Cefazolin, Gentamicin and Ciprofloxacin) using disc diffusion method according to the NCCLS guidelines. Results: ٍEscherichia coli (E. coli) was the most frequent microorganism which was isolated from 70.6% of peritoneal cultures, followed by Klebsiella pneumonia (13.7%), Pseudomonas aeruginosa (10.8%) and Proteus mirabilis (4.9%). E. coli which was the most common isolate was highly susceptible to ciprofloxacin (84.6%) and gentamicin (76.9%).The resistance rate of E. coli isolates to ampicillin was very high, i.e, 80.8% of E. coli isolates were resistant to ampicillin. The resistance rate for pseudomonas and Klebsiella spp. to commonly used antimicrobials varied from 25% to ciprofloxacin to 100% to ampicillin. Conclusion: E. coli was the most common isolate in post-operative peritoneal cultures. The results showed the relatively high resistance rate of the isolated microorganisms to commonly used antimicrobials, especially to ampicillin. So the choice of antimicrobial therapy must be based on the susceptibility tests and also take into account the risk of inadequate and in appropriate antimicrobial therapy and emergence of bacterial resistance to commonly used antimicrobials.
  Gholamreza Sepehri , Mohammad Pourranjbar , Ghollam-Reza Moshtaghi-Kashanian , Ali Siahposht Khachaki and Ehsan Sepehri
  Problem statement: There are controversies about the effects of anti-inflammatory drugs on exercised induced changes on plasma cytokines in untrained male athletes, so the primary purpose of this study was to measure the influence of a prophylactic dose of ibuprofen use on plasma cytokines during a 1500 m running race. Approach: Subjects were 10 untrained male Medical University students who consumed 400 mg ibuprofen two hours before the 1500 m running practice and 10 controls that competed in the race but avoided ibuprofen and all other medications. Blood samples were collected before, 1h and 24 h following the 1500 m exercise bout to measure plasma concentrations of interleukin-1 beta (IL-1β), interleukin-6(IL-6) and tumor necrosis factor alpha (TNF- α). Results: There were not any significant differences between plasma concentration of IL-1β, IL-6 and TNF- α during the study period. Also the data showed that there were not any significant differences between plasma level of IL-1β, IL-6 and TNF- α in ibuprofen and control group during the study period. Conclusions/Recommendation: Our result showed that a prophylactic dose of ibuprofen did not affect acute inflammatory cytokines levels (IL-1β, IL-6 and TNF- α) in untrained healthy young males. So the etiology of the muscle soreness in athletes actively participating in sport races seems to be independent of inflammatory processes.
  Gholamreza Sepehri , Vahid Sheibani , Afrooz Azarang , Ali Shamsizadeh , Mohammad Reza Afarinesh , Saeed Azizollahi and Ehsan Sepehri
  Problem statement: The present study was performed to determine the effect of Intracerebroventricular (ICV) administration of W-7, a specific calmodulin inhibitor, on the analgesic effect and development of tolerance to antinociceptive effect of acute and chronic morphine administration respectively. Approach: This study was carried out on male wistar rats, weighing 200-250 g. For acute experimental protocol, Morphine was injected intraperitonealy in a single dose (5 mg kg-1). For chronic experimental protocol, Morphine was administered daily (15 mg kg-1 for 8 days). The threshold to thermal nociceptive stimuli was measured by tail-flick test. In acute and chronic experiments, W-7 (0.25, 0.5 and 1 μmol/rat) was injected through ICV at different paradigms. Maximal Possible Effect percentage (MPE%) was considered as analgesia index. Results: Our result showed that W-7 (0.25, 0.5 and 1 μmol/rat) injections before acute morphine administration significantly reduced the analgesic effect of morphine compared with morphine treated group (all p<0.05). Chronic morphine exposure induced tolerance to its antinociceptive effect and administration of W-7 (0.5 and 1 μmol/rat) decreased the development of tolerance to it. Conclusion: In conclusion these data showed that acute administration of W-7 reduced the analgesics effect of morphine and chronic injection of W-7 inhibited the development of morphine tolerance which indicates that calmodulin and its dependent pathways may play a role in the morphine tolerance processes.
  Gholamreza Sepehri , Kayvan Yaghoobi , Vahid Sheibani , Ali Shamsizadeh and Mohammad Khaksari
  Problem statement: This study was performed to evaluate the effect of W7 (specific calmodulin inhibitor) on morphine induced analgesia in Adrenalectomized (ADX) rats by tail-flick test. Approach: Tolerance to morphine was induced in male Wistar rats by daily injections of morphine (15 mg kg-1, i.p) for 8 days. Adrenalectomy was performed under general anesthesia with intraperitoneal (i.p) injection of ketamine (50 mg kg-1) and xylazine (5 mg kg-1). In sham operated animals only the incision was made but adrenals were not removed. Five days after surgery, W7 (0.25, 0.5, 1 and 2 μmol rat-1) was injected Intracerebroventricularlly (ICV) concomitant with morphine (15 mg kg-1, i.p) for 8 consecutive days. Tail Flick Latency (TFL) was used to assess the nociceptive response at days 1, 3, 5 and 8 before and 30 min after morphine administration in sham operated and ADX rats. Maximal Possible Effect percentage (MPE %) was considered as analgesia index. Results: The results showed that daily morphine injection caused a marked analgesia in rats, but MPE % decreased significantly after 8 days which shows the development of tolerance to morphine (p<0.05). MPE % following morphine treatment in ADX rats was significantly greater than sham operated rats (p<0.05) and W7 (0.5, 1 and 2 micromol/rat/ICV) significantly attenuated the development of tolerance to morphine in ADX rats compared to sham operated rats (p<0.05 and p<0.001). Corticosterone replacement reversed the effect of W7 on ADX rats (p<0.005). Conclusion: The results of this study showed that hypothalamic- pituitary-adrenal axis and calmodulin may play a role in the development of tolerance to morphine antinociceptive effects in rats.
  Gholamreza Sepehri , Vahid Sheibani , Fariba Baghaiee and Rasoul Farazifard
  This study was performed to evaluate the effects of intra-nucleus accumbens (Nacc) shell microinjection of L-arginine (a NO precursor) and NG-nitro-L-arginine metyl ester (L-NAME (a Nitric Oxide Synthase (NOS) inhibitor) on morphine withdrawal signs in male rats. Wistar rats were anaesthetized with a mixture of ketamine and xylazine, and placed in stereotaxic apparatus and a guide cannula was inserted into Nacc shell, according to the atlas of Paxinos and Watson. Morphine dependency was induced by subcutaneous administration of morphine (20 mg kg-1 for 5 days), and morphine withdrawal signs were precipitated by naloxone administration (4 mg kg-1 i.p.). Rats were received either single or repeated microinjection of saline, L-arginine or L-NAME into Nacc shell during the scheduled periods. The results of this study showed no significant difference between control and saline treated groups in the expression of morphine withdrawal signs. Single dose microinjection of L-NAME/L-arginine, just prior to the last injection of morphine, had no effect on morphine withdrawal signs, but repeated microinjection of L-arginine/L-NAME decreased jumping, rearing and weight loss (only in L-NAME group), as compared to control rats. Present results indicate that NO in Nacc shell may be involved in some of morphine withdrawal signs.
  Gholamreza Sepehri , Vahid Sheibani , Fariba Baghaiee and Rasoul Farazi
  The rapid induction of dependency is the priority of injection methods, but high mortality rate due to medullary depression is the most important limitation of injection methods in laboratory animals. This study was carried out on 5 groups of adult male wistar rats weighing 250-350 g which were maintained in standard light and temperature with free access to food and water. Four methods in which morphine dependency was achieved during a 5 days period were chosen and the mortality rate and the time of mortality were compared with the new group which received morphine (10 mg kg-1 on the first day and 20 mg kg-1 from 2nd to 5th day). Precipitation of Morphine withdrawal signs was performed on the 5th days, 4 h after the last injection of morphine and withdrawal signs were recorded in 5 min intervals for 20 min. The results of this study showed that the new modified method is an effective method for induction of morphine dependency. The least mortality rate (19%) was observed in the new modified morphine dependency method. Mortality rate in other procedures were ranged from 33-60%. The results of this study showed that first dose of morphine as well as the pattern of increasing dosage regimen are important factors which determine mortality rate. So we propose that this new method would be suitable for rapid induction of morphine dependency.
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility