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Articles by Gheorghe Solcan
Total Records ( 2 ) for Gheorghe Solcan
  Alina Anton , Gheorghe Solcan and Carmen Solcan
  The effect of copper and zinc supplementation on serum haptoglobin and milk production performance was tested in a 20 weeks feeding trial with 40 Holstein-Friesian lactating cows, devided in 2 equal groups (supplemented and control). At 10-20 days post calving cows began receiving either a diet containing supplemental Cu and Zn sulphate (19.1±0.8 ppm Cu and 61.7±6.8 ppm Zn) or a diet without mineral supplement (3.1±0.4 ppm Cu, 9.2±1.1 ppm Zn). Milk and blood samples were collected six times every 28 days. Ten subjects of each group were randomly assigned to skin biopsies of the periorbital area at the end and the beginning of the study. Feeding inorganic Cu and Zn increased (p<0.001) plasma Cu and Zn values and significant interactions (p<0.001) was found between time and supplementation on plasma Cu and Zn levels. Serum haptoglobin values were lower (p = 0.24) in cows from supplemented group (0.096±0.006 g L-1) relative to the control group (0.099±0.009 g L-1). There was no effect of time (p = 0.21) and no significant interaction (p = 0.35) between time and supplementation on serum haptoglobin levels. The Body Condition Score (BCS) was higher in supplemented group than in those that did not receive supplemental minerals (2.82 vs. 2.75, p = 0.03) but no significant interaction was found between time and supplementation on BCS (p = 0.85). Feeding inorganic Cu and Zn reduced periorbital hypopigmentation in cows. Supplementing Cu and Zn comparing to the control cows had higher yield of milk (23 vs. 22 kg day-1, p = 0.004), milk crude protein (3.3 vs. 3.2%, p = 0.14), milk fat (4.16 vs. 4.09%, p = 0.002), milk lactose (4.82 vs. 4.79 g kg-1, p = 0.32) and milk energy (0.74 vs. 0.75 Mcal kg-1, p = 0.27) however, the response to supplementation tended to be inconsistent over time for production indicators as indicated by time x supplementation interactions (p>0.15). Supplemented group had lower SCC compared to the control cows (246,000 vs. 288,000 cells mL-1, p = 0.02) but no significant interaction was found between time and supplementation on SCC (p = 0.47). Supplementing with 1.5 g CuSO4A5H2O and 5 g ZnSO4A7H2O/cow/week for 1st 20 weeks of lactation can significant improve health and milk production performances in a deficient area.
  Carmen Solcan , Gheorghe Solcan , Bogdan Oprisan , Mihaela Spataru , Constantin Spataru and Viorel Floristean
  Aflatoxins are a significant threat to chicken welfare and production due to their presence in contaminated feedstuff. This study aimed to evaluate any immunosuppressive and proto-oncogenic effects of Aflatoxin B1 in experimentally dosed chickens. The 46 days old Ross 308 broiler chickens were treated with Aflatoxin B1 (50 µg kg-1BW diluted in sunflower oil, per os) for up to 28 days. Every 7 days, five treated and five control chickens were euthanized to determine changes in lymphoid organs, particularly and the thymus and bursa of Fabricius. Tissue changes were evaluated by conventional histology and electron microscopy as well as by immunohistochemistry for Bcl-2 expression. The Aflatoxin B1 exposure induced significant reductions in body weight and in the weight of the lymphoid organs at the end of the experiment. In parallel, severe thymocyte and lymphocyte depletion, considered due to apoptosis was evidenced in the thymus and bursa of Fabricius whereas reticulo-epithelial cells proliferated and strongly expressed the Bcl-2 protein. These results demonstrate the severe immunosuppressive effects of Aflatoxin B1 on B and T cells in chickens and its proliferative action on the reticulo-epithelial cells involved in lymphocyte maturation, suggesting a proto-oncogenic action.
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