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Articles by Ghasem Saki
Total Records ( 6 ) for Ghasem Saki
  Mohammad Reza Gholami , Ghasem Saki , Masoud Hemadi , Ali Khodadadi and Javad Mohammadi-Asl
  The purpose of this study was to test of efficacy of melatonin on the Optimizing of cryopreservation media in the testis tissue samples. Testes from neonate BALB/c mice were vitrified and then thawed under standard condition with or without the addition of 100 μM melatonin to both of vitrification and thawing solution. After that, Vitrified-thawed whole testes were digested under standard condition and subsequent viability of the cells in the suspension was analyzed using cytotoxicity kit and Apo-Brdu tunnel assay kit. The mean proportion of apoptotic testicular cells in the treated vitrified-thawed testes in comparison to no-treated ones was noted significantly (5.2±0.47 vs. 1.56±0.62, respectively). Moreover, melatonin cause decreasing the viability of the treated vitrified-thawed testicular cells in compared to no-treated vitrified-thawed testicular cells (4.78±0.46 vs. 8.39±0.76, respectively). In addition, the mean cytotoxicity of melatonin on the vitrified-thawed testicular cells was 9%. The associated reduction in healthy testicular cells in the treated vitrified-thawed testes suggests that melatonin in doses of 100 μM don’t protected testicular tissue from damaged induced in the process vitrification and thawing. However, further well-designed studies such as dosimetry melatonin and applied another cryoprotectants in the matching with melatonin are essential to offer a final conclusion.
  Fakher Rahim and Ghasem Saki
  As our knowledge no report was given about effect of leukemia inhibitor factor on sperm motility and survival rate of asthenospermic infertile men. That's why this study was decided to review the effects of Leukemia Inhibitor Factor (LIF) with different concentrations of 0, 3, 5, 10, 50 ng mL-1 on motility and survival rate of asthenospermic infertile men. Semen samples of 15 asthenospermic men who referred to IVF unit of Imam Khomeini Hospital, Ahvaz, Iran were collected and put in incubator under condition 5% CO2 in air at 37°C for 45-30 min then total sperm count was done first, then calculate the motile sperm with a degree a and b. In this study, we evaluated only samples that had sperm motility percent of more than 30%. After that time from every sample about 10 μL removed and culture in different media. Every drop was evaluated 6, 24, 48 h after cultured of sperm in it for motility and survival rate of sperm. Statistical analysis shows that the forward motility of sperm cultured for 6 h in media with and without of LIF is not significant (p>0.05) but after 24 h the forward motility and survival rate of sperm cultured in media with 10 and 50 ng mL-1 of LIF significantly increased (p<0.05) and after 48 h the forward motility and as well as survival rate of sperm cultured in media with 50 ng mL-1 of LIF significantly increased (p<0.05). The conclusion from this study is that certain concentrations of leukemia inhibitor factor can increase the motility and survival rate of sperm.
  Allahvaysi Ozra , Solaeymani-Rad Jafar , Lida Moradi and Ghasem Saki
  The aim of this study was to investigate ultrastructural changes of Cerebellum in 3mT electromagnetic field exposed rats. Total 30 adult female Wister rats with 3 months of age and weighing 210±10.6 g were used in this study. All female rats subdivided randomly to 2 groups: group 1, serve as untreated controls; group 2, was exposed to 3mT EMF for 4 months, 4 h day-1. After 120 days all rats were killed and their tissue samples from Cerebellum were removed and prepared for electron microscopic studies. Present finding clearly demonstrated that number of purkinje cells in the cerebellum of EMF- exposed rats were decreased significantly (p<0.01) in comparison to control group. The other changes include: condensation of nuclei, dilatation of endoplasmic reticulum, breakdown and disappearance of crista in mitochondria and vacuolization of cytoplasm in the purkinje cells of cerebellum. The mean nuclear diameter in purkinje cells were 45.35±22.85 mm and 26.79±16.36 mm in control and experimental group respectively. The statistical analysis showed that the difference between two group was significant (p = 0.03). Axial ratio of nucleus of purkinje in control and experimental groups were 1.86±0.41 and 1.55±0.14 mm, respectively. The axial ratio of nucleus in purkinje of EMF-exposed cerebellum were decreased significantly in comparison to control group (p = 0.02). These findings indicate that long-term exposure to EMF has detrimental effects on central nervous system at cellular level.
  Ghasem Saki , Fakher Rahim and Majied Jasemi Zergani
  The objective of this study was to evaluate whether cryopreservation of small volume of sample (sperm+cryoprotectant) was feasible using open pulled straw and also compared the outcomes of open pulled straw and conventional straw as carrier for normal human sperm cryopreservation. Semen samples were obtained from 10 men undergoing evaluation for infertility after 3-4 days of abstinence. Washed normal sperm samples were divided into three aliquots as follows: (1) Fresh; (2) cryopreserved in open pulled straw and (3) crypreserved in conventional straw. In order to do cryopreservation of sperm in open pulled straw first washed normal sperm samples were mixed with equal volume of test yolk buffer and 12% v/v glycerol, later on 3-4 μL from the prepared mixture was loaded in each straw by using syringe. The loaded straws were plunged into liquid nitrogen and after 3 months recovered and thawed. Each straw was emptied of their fluid content in drop of 10 μL medium covered with mineral oil. Motility of vitrified-thawing sperm was assessed by using inverted microscope. The results show as percent progress motility±SD and the p<0.05 were suggested as significant. The percent progress motility±SD of fresh sperm was evaluated as follow in study groups: For fresh group was evaluated as 59.2±7.6; the value of 37.5±8.2 in cryopreserved sperm in open pulled straws group; and value of 26.3±6.4 for conventional straw group, respectively. Statistical analysis shows that the difference between cryopreserved sperm in open pulled straws and conventional straw groups is significant (p = 0.001). Because of the significant difference between cryopreserved sperm in open pulled straws and conventional straw groups, we concluded that vitrification of human sperm is feasible using open pulled straw. The results of this study shows that open pulled straw could be a good carrier for cryopreservation of small volume of normal human sperm.
  Ghasem Saki , Fakher Rahim and lida Moradi
  Vitrification is the commonly used method for long-term storage of pre-implantation mammalian embryos. It is an essential part of assisted reproductive technologies. The re-expansion rate, pregnancy and birth rate of vitrified blastocysts using CPS were compared with OPS and Conventional Straw. Female NMRI mice were injected with Gonadotrophins in order induce them for super ovulation. At that time the mice were sacrified by cervical dislocation and dissected of mouse abdomen. The uterine horns were existed blastocysts were collected in PBS and randomly allocated to four groups: vitrification in CPS, conventional straw, OPS and untreated controls. The vitrification solution was EFS40%. After storage for 1 month in liquid nitrogen, the blastocysts were thawed in 0.5 M sucrose for in vitro culture in M16 medium. After 6 h of culture, the numbers of expanded blastocysts was recorded and ready for transfer to uterus of pseudo pregnant mouse. The re-expansion rate of the CPS group (72.1%) was significantly higher (p<0.05) than OPS (52.55) and C.S. (38.6%) groups. The pregnancy (70%) and birth rate (45%) of blastocysts in CPS were similar to those of fresh blastocysts (80% and 45.5%) and the pregnancy (10%) and birth rate (5.1%) in Conventional Straws lower than OPS (20 and 7.5%), but were not significantly different. Mouse blastocysts vitrified using CPS had a better result compared with OPS and Conventional Straw. The value of CPS for vitrification of blastocysts may also merit investigation.
  Maryamalsadat Jalali , Masoud Hemadi , Ghasem Saki and Alireza Sarkaki
  Noise stress is dangerous natural contaminant that produces harmful physiological, psychological and morphological outcomes to the body. So this study was conducted in order to investigate the effects of noise stress on the parenchyma of testis. Healthy mature females rats (n = 20) were mated with the mature male rats and then randomly allocated equally either to experimental or control groups. Experimental group has given daily noise stress up to birth their child. In the second step, the child's pregnant rats of experimental group were distributed to three subgroups as follow: group I (without exposure to noise stress), group II (exposure to noise for 8 weeks) and group III (exposure to noise for 14 weeks) for morphometric analysis of their child's testicles by sacrificing of them at weeks 14. In general, the testes of non-exposed group were grown larger than ones in the noise exposed groups. Moreover, the testes of the experimental group 1 were larger than the other experimental groups. Indeed, the rate of atrophic seminiferous tubules and jumbled appearance of the interstitial space were more observed in the noise stress exposed group than non-exposed ones. In addition, seminiferous tubules analysis revealed that the characteristics of interstitial space cells and epithelial germinative cells of the seminiferous tubules in the control group were better than the noise exposed groups. It seems that the noise stress has negative influences on the fertility of male based on enhancing of the apoptotic process induced by pathogenesis stress and suppressing the kinetics spermatogenesis.
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