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Articles by Gede Suantika
Total Records ( 4 ) for Gede Suantika
  Keukeu K. Rosada , Nur F. Afianti , Dea Indriani Astuti , Gede Suantika and Pingkan Aditiawati
  Bacterial communities in planktonic cells and biofilm at Saguling Hydro Power were investigated using Denaturing Gradient Gel Electrophoresis (DGGE) technique. Physicochemical characteristic of aqueous medium indicated that the water source was classified as moderately polluted (eutrophic). The study inferred that isolates in biofilm mostly derived from the planktonic cells and the Mann-Withney U tests showed that there was no significantly difference between the bacterial communities of planktonic cells and biofilm. The 16S rRNA sequences revealed that bacteria recovered from the planktonic cells were affiliated with Betaproteobacteria and Bacteroidetes phyla. Meanwhile, the sequences bacteria revealed from biofilm were closed to Betaproteobacteria, Alphaproteobacteria and Gammaproteobacteria groups. Overall, the majority of microbes identified from the two samples were belong to Betaproteobacteria group.
  Keukeu K. Rosada , Navisan Najia , Rahma Widya Ningrum , Dea Indriani Astuti , Gede Suantika and Pingkan Aditiawati
  Background and Objective: Biofilm plays an important role in causing microbial corrosion. One of prerequisites for microbes as energy source to grow and to form biofilm is carbon source. The purposes of this study were measuring the ability of biofilm community sampled from Saguling hydro power in utilizing carbon sources and assessing culturable heterotrophic of bacterial community from those biofilm. Materials and Methods: Biolog EcoPlateTM and culture-dependent approach were used to assess biofilm community. Results: Heterotrophic bacteria in biofilm have the ability to use 30 different carbon sources consistently. The source of carbon used by this community at the highest rate are N-acetyl-D-glucosamine from carbohydrates group, 2-hydroxybenzoic acid from carboxylic and kenotic acids, glycogen from polymers group and L-serine from the amino acid group at the rate of 0.16, 0.05, 0.14 and 0.09 absorbance U h–1 respectively. Whereas the carbon sources with high consumption are as follow: Pyruvic acid methyl ester, β-methyl-D-glucoside, D-mannitol and N-acetyl-D-glucosamine from carbohydrates group; D-galacturonic acid, 2-hydroxybenzoic acid, D-glucosaminic acid and D-malice acid from the group of carboxylic and kenotic acids; tween 40 and 80 as polymers; L-argentine, L-asparagines, L-serine, L-heroine and glycol-L-glutei from amino acids group. Furthermore, culturable bacterial community of those biofilm were dominated by Gram-negative bacteria, consisted of five common/heterotrophic bacteria, two manganese bacteria, two nitrifying bacteria, three iron bacteria and three sulfate reducing bacteria. Conclusion: Bacterial community of biofilm from Saguling hydro power which were dominated by Gram-negative bacteria have the ability to use various carbon sources and degrade glycogen.
  Gede Suantika , Alissa Diany Putri , Yovita Astuti Djohan , Fahma Fiqhiyyah Nur Azizah , Dea Indriani Astuti and Pingkan Aditiawati
  Background and Objective: The impact of environmental factors on the B vitamins (B1, B2, B6 and B12) content of marine diatom Skeletonema costatum has not yet been evaluated so far. Here, we aim to optimize the B vitamins production (vitamin B1, B2, B6 and B12) through culture of S. costatum on different salinity levels as well as light intensity exposures. Materials and Methods: The marine diatom Skeletonema costatum was cultured in different salinity levels (24, 29 and 34 g L–1) to optimize B vitamins production, prior to exposure to different light intensity levels (20, 34 and 47 μmol m–2 sec–1) for 3 days in f/2 medium. Results: Twenty four grams per liter salinity exposure produced the highest vitamin B1, B6 and B12 content of 90.08±2.6, 410.03±12.97 and 61.22±27.67 μg g–1, respectively. When cultivated either below or above light intensity of 34 μmol m–2 sec–1, vitamin B1, B6 and B12 content decreased. The highest total B vitamins obtained at 34 μmol m–2 sec–1 was 563.46 mg L–1 (vitamin B1: 90.08±1.48 μg g–1, vitamin B2: 2.87±0.79 μg g–1, vitamin B6: 410.03±12.97 μg g–1 and vitamin B12: 61.22±27.67 μg g–1). Conclusion: Optimum growth, biomass and total B vitamins produced was achieved by culturing at salinity of 24 g L–1 and light intensity 34 μmol m–2 sec–1. So far, optimum growth, biomass and total B vitamins produced was achieved by culturing at salinity of 24 g L–1 and light intensity 34 μmol m–2 sec–1. Note that when aiming at high vitamin productivities, it is better to culture S. costatum in a two step process: A nutrient sufficient phase in optimum environmental growth conditions to produce enough cells (e.g., salinity, illumination, pH, temperature, supply of CO2 and nutrients etc.), followed by suitable stress to stimulate B vitamins synthesis in a controlled manner.
  Gede Suantika , Magdalena Lenny Situmorang , Pingkan Aditiawati , Abdul Khakim , Shrikumar Suryanarayan , Sri Sailaja Nori , Sawan Kumar and Ferisca Putri
  Background and Objective: Several seaweeds have been reported to contain different bioactive compounds with antimicrobial activity, providing protection against certain infectious diseases in aquaculture production. This study aimed to explore the potential of red seaweed Kappaphycus alvarezii product as an alternative for anti-infective strategy and enhancement of salinity stress tolerance in shrimp (Litopenaeus vannamei) hatchery culture. Materials and Methods: Shrimp post-larvae were fed with Artemia nauplii, either enriched or not enriched with seaweed paste then challenged with Vibrio at day 5 and 8. Comparison of shrimp growth and survival between treatments following salinity stress test and Vibrio challenge were done using one-way analysis of variance analysis. Results: Shrimp fed with seaweed-enriched Artemia resulted in higher survival after Vibrio challenge (90.2±7.0%) compared to shrimp fed with non-enriched Artemia (77.7±3.1%). Shrimp fed with non-enriched Artemia resulted in lower growth after Vibrio challenge (9.65±0.20% b.wt., day–1) compared to the non-challenged group (10.34±0.25% b.wt., day–1). In contrast, there was no difference in the growth of shrimp fed with seaweed-enriched Artemia with or without Vibrio challenge (10.51±0.19 or 10.80±0.28% b.wt., day–1, respectively). The shrimp fed with seaweed-enriched Artemia also obtained a higher survival following salinity stress test (94±2%) compared to shrimp fed with non-enriched Artemia (79±4%). Conclusion: Overall results suggested that red seaweed K. alvarezii by-product enrichment on live feed Artemia for shrimp post-larvae during the hatchery phase can provide protection against V. harveyi infection, improve the growth of shrimp when exposed to pathogenic V. harveyi and also allow higher salinity stress tolerance. Further evaluation on the effect of seaweed by-products dietary supplementation in the nursery and grow-out phases are undoubtedly required, to accurately evaluate the potential of seaweed by-product application as a growth and disease resistance promoting agent in those later phases as well.
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