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Articles by Gebin Li
Total Records ( 3 ) for Gebin Li
  Nobuko Mori , Gebin Li , Megumi Fujiwara , Shingo Ishikawa , Koh Kawasumi , Ichiro Yamamoto and Toshiro Arai
  The prevalence of obese cats has increased because of over calorie diet and physical inactivity. Obesity has been found to be associated with oxidative stress and Reactive Oxygen Species (ROS). Unfortunately oxidative stress status at the early phase of obesity in high fat fed cats is not well understood. The objectives of this study were (1) To evaluate lipid and glucose metabolism using enzymatic, hormonal and oxidative stress biomarkers at the early obese phase of cats fed on a high-fat diet and (2) To identify rapidly changing variables to use as a diagnostic marker for lipid metabolic disorders in cats. Total 13 domestic female cats were divided into two groups which were fed on control and high-fat diet for eight weeks, respectively. After the feeding period, they were compared in metabolic variables and oxidative stress markers in plasma and tissues. As results, High-fat diet including much long chain fatty acids promoted rapid changes in lipid metabolism, particularly accelerated β-oxidation of fatty acids and oxidative stress in the liver of the cats. G6PD, GPx and SOD were increased in the liver. Insulin resistance was not apparent at the early phase of obesity in cats. Plasma activities of SOD also increased at the early phase of obesity in cats. Remarkable alternation for oxidative stress in liver was observed at the early phase of obesity in cats fed on high fat diet and SOD may be a potential marker of the early phase of obesity in cats.
  Gebin Li , Peter Lee , Yuki Okada , Ichiro Yamamoto , Toshiro Arai and Nobuko Mori
  The objective of this study was to determine whether Peripheral Blood Leukocytes (PBL) are sensitive enough to detect early signs of diet induced obesity related changes occurring in insulin sensitive tissues, such as abdominal omental and subcutaneous adipose, liver and skeletal muscle, by comparing transcriptome profiles of insulin signaling (IRS-1, IRS-2 and PI3-K p85 α), adiponectin signaling (AdipoR1 and AdipoR2), energy homoeostasis (G6PDH and MDH) and sterol metabolism (FASN) genes as determined by RT-PCR in cats fed on High Fat (HF) diet. Regarding PBL concordance, using a HF diet induced obesity cat model, out of seven genes examined, concordance was observed with ~60% (5 out of 8) of them (IRS-1, IRS-2, Adipo-R1, Adipo-R2 and MDH) between PBL and tissue transcriptomes. HF diet cat PBL IRS-1 and IRS-2 mRNA expression were both reduced, when compared to control diet which was in concordance with reduced IRS-1 and IRS-2 mRNA expression in both abdominal and subcutaneous adipose of HF diet cats. Similar to IRS-1 and IRS-2, AdipoR1 and AdipoR2 mRNA expression in HF diet cats was also reduced, when compared to control diet which was in concordance with reduced AdipoR1 and AdipoR2 in liver and skeletal muscle, respectively of HF diet cats. Lastly, PBL MDH mRNA expression was reduced and was concordant with reduced mRNA expression in liver and skeletal muscle. Overall, our results demonstrate that PBL are sufficiently sensitive to high fat diet induced alterations to transcriptomes of insulin sensitive tissues and can serve as surrogate tissue for various insulin sensitive tissues.
  Gebin Li , Peter Lee , Ichiro Yamamoto , Nobuko Mori and Toshiro Arai
  Peripheral Blood Leukocytes (PBL) continually interact with virtually every organ and tissue in the whole body. A remarkable concordance (80%) of gene expression profiles between peripheral blood mononuclear cells and different tissues has been previously demonstrated in humans. As such, gene expression responses of circulating PBL can therefore, potentially provide early warning of any abnormalities they discover. Weight alterations (increase or decrease) when associated with obesity has been reported to lead to alterations to PBL gene expression, especially those related to insulin and adiponectin signaling genes and even genes involved with energy homeostasis. As such, a pilot study involving PBL profiles of the following genes involved in energy homoestasis (5’ Adenosine Monophosphate-Activated Protein Kinase (AMPK)-α1 and 2, -β1 and 2 and -γ1 and 2); Glucose-6-Phosphate Dehydrogenase (G6PDH) and Malate Dehydrogenase (MDH), lipogenesis (Fatty Acid Synthase (FAS) and insulin signaling Adiponectin Receptor (ADIPOR) (-1 and 2); Insulin Receptor Substrates (IRS) (-1 and 2); Phosphatidylinositol-3 Kinase (PI3-K) were evaluated between lean and overweight dogs in an attempt to identify possible PBL biomarkers for assessing obesity in dogs. As compared to lean dogs, overweight dog PBL demonstrated reduced mRNA expression of IRS-1, IRS-2, FAS, G6PDH and AMPK β1 genes. Overall, these findings suggest that dysregulation of energy metabolism, associated with obesity in overweight dogs may carry over with alterations in PBL gene expression of genes involved in energy homeostasis and sterol metabolism. As such, PBL gene expression profiles may aid in early detection of PBL biomarkers for assessing obesity in dogs.
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