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Articles by Gautam Patra
Total Records ( 5 ) for Gautam Patra
  Gautam Patra , M. Ayub Ali , Kh. Victoria Chanu , L. Jonathan , L.K. Joy , M. Prava , R. Ravindran , G. Das and L. Inaotombi Devi
  The present investigation was undertaken to evaluate histo-pathological, haemotological and biochemical changes in broiler chicken naturally infected with Eimeria tenella. A Polymerase Chain Reaction (PCR) based assay was also done for the detection, identification and differentiation of pathogenic species of Eimeria in poultry. Post mortem examination revealed petechial haemorrhages, oedema, necrosis and sloughing of caecal epithelium. Histopathological evidence showed leakage of blood, oedema and necrosis. Haemotological studies revealed that coccidial infection caused significant decrease in the value of haemoglobin and Packed Cell Volume (PCV). The value of Mean Corpuscular Volume (MCV) and Mean Corpuscular Haemoglobin Concentration (MCHC) revealed that caecal coccidiosis resulted in macrocytic hypochromic anaemia. Biochemical serum analysis of coccidial infected chicken showed a significant increase in level of glucose, Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) while there was a significant decrease in the level of alkaline phosphatase (SAP) and cholesterol. In PCR using specific primers for E. tenella only the ITS 1 regions of ribosomal DNA (rDNA) could be amplified.
  Irungbam Karuna Devi , Y. Geeta Devi , Kh. Victoria Chanu , M. Ayub Ali , Jully Gogoi Tewari , M. Prava , David Malsawmkima and Gautam Patra
  The present work describes isolation, cell wall preparation, morphological as well as molecular detection of oocyst of Eimeria tenella. Based on transmission electron microscopy, the oocyst of this species showed the outer and inner wall without veil forming bodies; cross section of sporozoites with different organelles and macrogamete structure at ultrastructural level. Polymerase Chain Reaction (PCR) based on Internal Transcribed Spacer 1 (ITSI) regions of ribosomal DNA (rDNA) demonstrated 270 bp specific for E. tenella. Results of this study provide the basis for further ultrastructural studies of the organism. The present study also suggested that morphological characteristics along with molecular identification are prerequisite for validation and identification of Eimeria species.
  Gautam Patra , W.M. Lyngdoh , M. Ayub Ali , M. Prava , Kh. Victoria Chanu , T.C. Tolenkhomba , Gunjan Das , H. Prasad , L. Inaotombi Devi and Irungbam Karuna Devi
  A study was carried out in broiler chicken for comparative anthelmintic efficacy of neem and pineapple leave powder with piperazin hydrate for treatment of experimental Ascaridia galli infection. The affected birds had lower levels of Hb, PCV, Ca and P. The profile changed after treatment with neem and pineapple leave powder which was comparable to that observed after treatment with piperazine hydrate. Neem and pineapple leave powder treatment caused cent percent evacuation of the worms on 28th and 56th day post treatment respectively.
  H. Lalliankimi , Gautam Patra , Lalnuntluangi Hmar , Zosangpuii and Rody L. Fanai
  Background and Objective: Zinc (An essential trace element) has an important function in metabolic activities such as protein synthesis, carbohydrate metabolism, reproduction, growth and a variety of biochemical reactions. The zinc ion is a co-factor of many enzymes and a component of metalloenzymes that participates in a number of metabolic processes. In quails, the deficiency of zinc is characterized by slow growth, abnormal feathering, labored respiration, in-coordinated gait, loss of appetite, decrease in egg production and reproductive performance, problems in bone and skin development and also increased mortality. The aim of this study was to determine the growth performance and egg quality of Japanese quails under different levels of zinc supplementation. Methodology: In order to carry out the experiment, a total of 400 days-old quail chicks were randomly selected and sub-divided into four groups i.e., C, T1, T2 and T3 having hundred quails with 10 replicates having 10 quail chicks in each which were supplemented with zinc oxide powder at the rate of 0, 25, 50 and 75 mg kg–1 of diet respectively. The quails were reared under deep litter system using paddy husk as litter material. The birds were fed twice daily at 6:00 am in the morning and 4:30 pm in the afternoon with basal diet of broiler pre-starter and starter rations containing 22 and 18 mg of zinc, respectively. Small portions of the basal diet were first mixed with the respective amount of zinc oxide powder; this small amount was then mixed with a larger quantity of the basal diet until the total quantity was homogenous. Results: The inclusion of zinc in the diet had no significant effect on growth performance in terms of day old body weight, 5th week body weight and daily gain. Zinc supplementation was found to have significant effect in T1, T2 and T3 on albumen index but no significant effects were observed among treatment groups with respect to egg weight, shape index, yolk index, yolk %, albumen %, shell weight % and Haugh unit. Conclusion: The results showed that zinc supplementation at the rate of 50 mg kg–1 of feed and 75 mg kg–1 of feed have significant effect on the albumen index but not on other quality parameters or on body weight gain in terms of day old body weight, 5th week body weight as daily gain.
  Gautam Patra , M. Ayub Ali , Kh. Victoria Chanu , Jonathan Lalsiamthara , J.L. Kataria , Snigdha Hazarika , David Malsawmkima , R. Ravindran and L. Inaotombi Devi
  An outbreak of coccidiosis in laboratory rats has been described in this study. Clinically, out of 50 rats, 30 were died after showing symptoms of anorexia, emaciation and diarrhea. Post mortem examination showed the large and small intestine distended with necrotic and sloughed off mucosal epithelial cells with intestinal content. The examination of the gut contents revealed numerous Eimeria oocysts. After sporulation, the oocysts were identified as those of Eimeria nieschulzi. The infection due to Eimeria nieschulzi is confirmed by Polymerase Chain Reaction (PCR) after amplifying partial (~1.6 kb) nuclear 18S rDNA from DNA of E. nieschulzi. The histopathological examination showed severe damage of the lamina propria of the intestinal mucosa with numerous coccidian developmental stages in the epithelium of small intestine. Biochemical analysis of serum from infested rats presented a significant increase in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), glucose, total proteins, globulin and inorganic phosphorous while a decrease in alkaline phosphatase and blood urea nitrogen with insignificant changes in potassium, sodium, magnesium and chloride.
 
 
 
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