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Articles by G.Y. Liu
Total Records ( 2 ) for G.Y. Liu
  G.Q. Liu , X.P. Jiang , J.Y. Wang , Z.Y. Wang , G.Y. Liu and Y.J. Mao
  Genetic variation at 20 microsatellite loci and genetic diversity were examined for Yangzhou Chicken. Observed and effective number of alleles across the microsatellite loci varied from 2-6 with an overall mean of 3.778 and 2.404, respectively. Observed and effective heterozygosity varied from 0.129-0.755 with an average of 0.422 and 0.517, respectively. Average polymorphism information content was 0.464. The genetic structure indicated that Yangzhou Chickens have substantial genetic variation. Population showed fairly high level of inbreeding (Fis = 0.184) and global heterozygote deficit. The allele frequency distribution is displayed an L shape, suggesting that no recent bottleneck affecting the genetic variability occurred. The information generated in this study will greatly aid in the establishment of effective breeding strategies for Yangzhou Chicken and may further be utilized for studying differentiation and relationships among different chicken breeds.
  G.Y. Liu
  The complete CDS sequences of three goat genes-goat RAB14, RB11B and SOD3 were amplified using RT-PCR. Sequence analysis of these three genes revealed that the goat RAB14 gene encodes a protein of 215 amino acids and has high homology with the Ras-related protein Rab-14 (..RAB14) of seven species-rat and pig (100%) human, mouse, chicken and orangutan (99%), dictyostelium discoideum (71%). The goat RB1B gene encodes a protein of 218 amino acids and has high homology with the Ras-related protein Rab-11B (RB11B) of five species-human, rat and bovine (99%), mouse (98%), electric ray (97%). The goat SOD3 has high homology with the superoxide dismutase 3, extracellular (SOD3) of four species-rabbit (74%), human (74%), rat (61%) and mouse (60%). The phylogenetic tree analysis revealed that the goat RAB14 gene has a closer genetic relationship with the RAB14 gene of rat and pig and the goat RB1B gene has a closer genetic relationship with the RB11B of human, rat and bovine. The goat SOD3 gene has a closer genetic relationship with the SOD3 of rabbit. The gene expression profile analysis indicated that the goat RAB14, RB11B and SOD3 genes were differentially expressed in tissues including ovary, pituitary, muscle, kidney, heart, lung, liver and spleen. The experiment established the primary foundation for further research on these three goat genes.
 
 
 
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