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Articles by G.M. Nava
Total Records ( 2 ) for G.M. Nava
  R.L. Jarquin , G.M. Nava , A.D. Wolfenden , A.M. Donoghue , I. Hanning , S.E. Higgins and B.M. Hargis
  An Organic Acid Mixture (OAM) was evaluated for efficacy against Salmonella enteritidis (SE) horizontal transmission and crop colonization in broiler chickens. The biocidal efficacy of the OAM (0.024% tannic, 0.042% lactic, 0.048% butyric and 0.048% acetic), was initially determined in vitro by treating a feed suspension inoculated with SE. The OAM was effective at reducing SE by at least 1 log unit in the feed suspension (P = 0.05). Treatment with the OAM was also assessed in combination with a probiotic treatment to evaluate effectiveness against horizontal transmission. One hundred newly hatched chicks were administered 3 treatments of OAM or probiotic, or 3 treatments of both OAM and probiotic over a 10 d period. Chicks (n = 20/group) were orally challenged with a 105 cfu mL-L culture of SE prior to treatment to act as sources for horizontal transmission. Ceca were collected and cultured for SE after 10 or 20 d. Horizontal transmission was reduced with OAM treatment solely (P = 0.05) or in combination with probiotic (P = 0.05). However, no apparent advantage to using both treatments was observed. The ability of the OAM to reduce SE crop contamination was determined by challenging market aged broilers with a 109 cfu mL-L culture of SE and administering the OAM in drinking water. Broilers were challenged with SE and immediately given the OAM or given the OAM 2 d prior to SE challenge. OAM was valuable in preventing horizontal transmission but did not eliminate crop colonization. The emergence of SE strains resistant to multiple antibiotics presents the need for alternative treatments and the results of these experiments show that an OAM may be a cost efficient and effective tool however the method of application of the OAM may alter effectiveness.
  G. Tellez , G.M. Nava , J.L. Vicente , M. De Franceschi , E.J. Morales , O. Prado , J.C. Terraes and B.M. Hargis
  A trial was conducted to evaluate the influence of dietary Aspergillus Meal (AM) prebiotic on intestinal development, morphology in turkey poults. Day-old Nicholas poults (n = 100) were randomly assigned to two groups. Poults in each group were divided and placed in floor pens with 50 poults each. One group served as a control and received a ration containing no added AM prebiotic. Poults in the treatment group were provided a ration supplemented with 0.2% AM prebiotic. Each dietary regimen and water were provided ad libitum to 30 days of age. Poults were humanely killed by CO2 inhalation and specimens of duodenum and distal ileum were collected at 10, 20 and 30 days. Histology sections were cut (5 μm) and stained with haematoxylin and eosin and combinations of either of periodic acid-Schiff with alcian blue, or high iron diamine with alcian blue stains to evaluate 20 intact villi on each section. The morphometric variables analyzed included villi height, villi surface area and crypt depth. In addition, goblet cells, classified as neutral, acidic or sulpho mucin cells, respectively, were quantitative for each treatment group. At all times of evaluation, AM prebiotic significantly increased the number of acid mucin cells in the duodenum, neutral mucin cells in the ileum and sulpho mucin cells in the duodenum and ileum. Villi height and villi surface area of both duodenum and ileum were significantly increased at days 10 and 30 compared to control. The present study suggest that AM prebiotic has an impact on the mucosal architecture and goblet cells proliferation in the duodenum and ileum of neonate poults.
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