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Articles by G.H. Zhao
Total Records ( 3 ) for G.H. Zhao
  G.H. Zhao , W. Cheng , P.J. Zhang , Y.S. Han and D.K. Chen
  The present study analyzed genetic variation and genotypes of 17 strains of type 2 Porcine Circovirus (PCV2) from different epidemic regions in China in 2008 and 2009. All the genomic sequences were 1.767 bp in length. Sequence comparison of complete genomic sequences revealed 95.6-99.9% identity among 17 PCV2 strains and the most variable regions within 1.000-1.700 nt (located in the coding region of ORF2). Comparative analysis of amino acids of the two ORFs revealed that variation extend of ORF2 (93.1-100%) was greater than ORF1 (98.4-100%) and the third codon position showed much more variable than the first and second sites. Mutations in T and B lymphocyte epitopes were also detected by comparative analysis and it was found that T lymphocyte epitopes were more conserved than those of B lymphocytes. Phylogenetic analysis revealed 6 novel genotypes of PCV2 in addition to the 5 known geneotypes (PCV-2a, PCV-2b, PCV-2c, PCV-2d, PCV-2e) reported. Of these genotypes, the PCV-2b, PCV-2d and 3 unidentified genotypes were the most prevailing, within 13, 17 and 16 epidemic provinces, respectively. For the 17 Chinese PCV2 strains examined in this study, 5 strains represented PCV-2b genotype, 6 strains were PCV-2d, 1strain was PCV-2e and other 5 strains were novel genotypes while no strains were PCV-2a and PCV-2c genotype. These findings demonstrated the usefulness and attributes of complete genomic sequences for genetic variation and genotyping of PCV2 and have implications for the studies of population biology, molecular epidemiology and genetic structure of PCV2 and for the effective control of PMWS as well.
  C.Y. Wu , G.H. Zhao , Y.Q. Zhao , H. Liu , P.J. Zhang and D.K. Chen
  CD4+CD25+ T cells played a critical role in the establishment and maintenance of peripheral tolerance via adoptive transfer. However, whether one or more molecules in CD4+CD25+ T cells that could independently mediate peripheral tolerance was disputed by worldwide researchers. In the present study, one soluble antigen-specific factor was extracted from splenic lymphocytes lysates of OVA-tolerant mice (named OVA Immune Tolerance Factor, OVA-ITF) with molecular mass <3 ku which could establish OVA-specific immune tolerance in recipient mice via transfer treated and induce the same effect of peripheral tolerance as those of splenic lymphocytes from OVA-tolerant mice. Treated with OVA-ITF to naive BALB/c mice resulted in significant suppression of DTH reaction and T cell proliferation in an antigen-specific manner as well as a significant increase in the percentage of CD4+CD25+ T cells within the CD4+ T cell subset in peripheral blood. Present study showed that OVA-TIF was produced by CD4+CD25+ T cell subset and could induce OVA-specific peripheral tolerance independently in vivo with TGF-β1 as its main suppressive cytokine in recipient mice. These results suggested that OVA-TIF is a novel, low MW factor and totally different from other suppressive components reported previously which have important implications for expanding new potential therapeutic routes of prevention and control of graft rejection, autoimmune and related diseases.
  F. Chen , J. Li , H. Sugiyama , Y.B. Weng , F.C. Zou , R.Q. Lin , Z.G. Yuan , H.Q. Song , X.Q. Zhu and G.H. Zhao
  In the present study, a portion of the 18S and 28S ribosomal DNA (rDNA) sequences of 35 Schistosoma japonicum isolates representing three geographical strains from mainland China, the Philippines and Japan were amplified and compared and phylogenetic relationships were also reconstructed by Unweighted Pair-Group Method with Arithmetic averages (UPGMA) using combined 18S and 28S rDNA sequences as well as the corresponding sequences of other species belonging to the Schistosoma genus available in the public database. The results indicated that the partial 18S and 28S rDNA sequences of all S. japonicum isolates were 745 and 618 bp, respectively and displayed low genetic variation among S. japonicum strains and isolates. Phylogenetic analysis revealed that the combined 18S and 28S rDNA sequences were not able to distinguish S. japonicum isolates from three geographical origins but provided an effective molecular marker for the inter-species phylogenetic analysis and differential identification of different Schistosoma species.
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