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Articles by G.A. Brockmann
Total Records ( 2 ) for G.A. Brockmann
  S.M. Abu Sara , A. Said Ahmed , M.K.A. Ahmed , M. Reissmann , G.A. Brockmann and S.A. Rahmatalla
  The aim of the study was the characterization of DGAT1 variants in Sudanese dairy cattle breeds. In this study, we examined 94 Kenana and 91 Butana dairy cattle from two regions of Sudan. We genotyped the DGAT1 sequence variant AJ318490.1:g.10433/10434 AA>GC that leads to the Lysine-Alanine substitution at position 232 (K232A) in the protein and the VNTR polymorphism in the promoter region. Genotyping was performed by allele specific PCR and PCR fragment lengths determination, respectively. In both breeds, the DGAT1 Lysine variant (232K) that is associated with high fat and protein content as well as high fat yield in other breeds is the high frequent allele. The frequencies of the 232K allele were 96.3 and 84.6% in Kenana and Butana breeds, respectively. At the DGAT1 promoter VNTR locus, four alleles containing four to seven repeats of the 18 bp motif were found in both breeds. The highest frequent allele was the VNTR allele 3 containing five repeats with 60.4 and 57.5% in Kenana and Butana breeds, respectively. In conclusion, the two examined Sudanese dairy cattle breeds do not differ in allele frequencies at the DGAT1 locus.
  S.A. Rahmatalla , M. ReiBmann , U. Mueller and G.A. Brockmann
  The aim of study was to investigate the genetic effects of candidate genes for milk production (Diacylglycerol Acyltransferase 1 (DGAT1), Leptin (LEP), kappa casein (CSN3), Growth Hormone Receptor (GHR), Osteopontin (OPN/SPP1) and ATP Binding Cassette subfamily G member 2 (ABCG2)) in Sudanese dairy cattle breeds. We examined forty Butana and twenty Kenana cows for which milk production data were available from two Sudanese research stations. In the DGAT1 K232A, allele 232 K was most frequent with frequencies of 95.31 and 79.17% in Butana and Kenana breeds, respectively. The DGAT1 K232A genotypes showed significant effects on milk yield (p = 0.0124), fat content (p<0.0001) and protein yield (p = 0.0361). The allele substitution effect of the Alanine variant (232A) was significantly higher milk yield (2.45±0.20 kg, p<0.0001) and protein yield (0.074±0.01 kg, p<0.0001) compared to lysine variant. For the DGAT1 promoter VNTR, three different alleles (3, 4 and 5) were found in Butana cattle and two (3 and 4) in Kenana cattle. Allele 3 was the highest frequent allele of 70.5 and 81.3% in Butana and Kenana breeds, respectively. This allele 3 increased both fat (0.84±0.22%, p = 0.0013) and protein (0.19±0.08%, p = 0.0333) contents. The same trend was observed in Kenana cattle. For the Mbo1-RFLP in the LEP gene, the allele A was almost fixed with frequencies of 97.50 and 97.06% in Butana and Kenana cows, respectively. The AB carriers had higher milk, protein and fat yields when compared with homozygous AA cows in Butana and Kenana cattle. With respect to CSN3, allele A was the major allele with a frequency of 86.25 and 89.29% for Butana and Kenana cows, respectively. The CSN3 polymorphism was not significantly associated with milk yield and composition. No variation was found for the examined SNPs with GHR, OPN and ABCG2 genes. The results of the present study provide evidence that polymorphisms in the DGAT1, Lep and CSN3 genes that segregate in Holstein Friesian dairy cattle also segregate in Butana and Kenana cattle and the direction of effect is the same as in Holstein Friesian cattle. These results provide a potential for genetic selection of animals with a predisposition for high milk yield, fat and protein yield in Sudanese cattle. The improve productivity (milk yield) in Sudanese cattle; selection of the minor allele could be enhanced to increased the milk production for these breeds.
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