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Articles by G. Seshagiri Rao
Total Records ( 2 ) for G. Seshagiri Rao
  P.V. Joshua , D.R. Salomi Suneetha , A. Arundhati and G. Seshagiri Rao
  Present study tends to investigate the existence of the bacterial oncogenes- rol genes of Agrobacterium rhizogenes Riker., in Nicotiana rustica, N. plumbaginifolia and their hybrid. These Nicotiana species and hybrid were normal morphologically under field conditions without any neoplastic growth. So, the habituation studies were carried out to identify the presence of rol genes by culturing the leaf discs on hormone supplemented Murashige and Skoog medium and then shifting to MS basal medium. Habituation showed phytohormone independent growth response even after shifting to MS basal medium indicating the presence of rol genes. Then, Polymerase Chain Reaction analysis showed amplification of rol A, B and C genes in N. rustica, rol A and C in N. plumbaginifolia and the hybrid. The amplified genes were sequenced and deposited in GenBank. The homology between the novel genes was compared with the rol genes of A. rhizogenes as well as the other plants species containing rol gene homologues using Pairwise sequence alignment search tool. Results were discussed in terms of the occurrence of bacterial genes in plants, the extent of their expression and the probable reasons for silencing of the foreign genes.
  Ch. Surekha , A. Arundhati and G. Seshagiri Rao
  Stable transformation followed by regeneration and subsequent expression of genes is important in the production of transgenic plants. Plumule and nodal segments of embryo, cotyledons and nodes from 10-12 day old seedlings of pigeonpea genotypes were cocultivated with different strains of Agrobacterium tumefaciens containing Ti plasmid and gus reporter gene. Transformation frequencies measured as number of explants showing gus expression was studied. It was observed that the transformation frequency is explant, genotype and Agrobacterium strain dependent. The transformation frequency was higher in ICPL87 and nodal explant in all genotypes studied. Agrobacterium strain LBA4404 was more compatible in giving higher frequency of transformants with all explants of all genotypes.
 
 
 
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