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Articles by G. Saki
Total Records ( 15 ) for G. Saki
  G. Saki , M. Hashemitabar , M. Abdollahi and S.H. Razie
  The aim of this study was to evaluate the effects of Vero cells on early embryonic cleavage rate and overcome cellblock of mice embryos in vitro. Female mice were super-ovulated by Intra-peritoneal injection of 5 IU Pregnant mare serum gonadotropine (PMSG) and 5 IU Human Chorionic Gonadotropine (HCG) 48 h later. The super-ovulated female NMRI mice placed individually with NMRI (Noda Medical Research Institute) males of proved fertility. The following morning, the females with positive vaginal plug were killed and cumulus-enclosed single cell embryos (2PN) were recovered. Two pronuclear (n=170) embryos were divided randomly into 2 groups: (1) co culture with vero cells (2) culture in simple culture medium. The rate of the development and the morphological appearance of mouse embryos in two groups were recorded daily for 120 h after retrieval in each system using inverted microscope. On day 5 of development the results showed that embryos cultured on vero cells had a significantly higher blastocyst and hatching formation rate than those in simple culture medium alone (p<0.05). It is concluded that Vero cells may improve mouse embryo development partly by increasing blastocyst formation, hatching blastocyst rate.
  F. Rahim and G. Saki
  This study taking to account the limitations of studies in this area, aimed to compare the survival rate, fertilization capacity and developmental capacity of oocyte that cryo-preserved in open pulled and conventional straw. Total 90 females NMRI mice were used in this research. First the male mice were scarified then the caudal part of epididymis was isolated. By creating cuts in caudal part of epididymis, sperms were isolated and put in T6 medium + 5 mg mL-1 BSA. Later on the sperms were incubated under 37°C and 5% CO2 for 2 h. Then sperm were added to living control, cryo-preserved in conventional and open pulled straws oocytes. After 4-6 h changing medium, two nuclear and two-cell embryos were evaluated 8 and 24 h after addition of the sperms, respectively. Comparing the two groups shows that the vitrified oocyte of open pulled straw method had better and significant different survival rate than conventional method (p = 0.001). Furthermore, between open pulled group and conventional group also there was significant different in term of fertilization rate (p = 0.001). The developmental capacity of open pulled straw group was significantly higher that conventional straw group (p = 0.002). Oocyte cryo-preservation, if consistent and successful, offers a way to avoid the complications of routine IVF therapy. Oocytes may need to be cryo-stored in the event of unforeseen non-production of sperm during IVF therapy, allowing a more measured consideration of donor sperm use or other means of sperm retrieval.
  S. Nikakhlagh , F. Rahim , G. Saki , A. Khosravi , H. Rekabi and N. Saki
  The aim of this retrospective study was investigating the anatomical position and causes of deep neck infections as well as detection of the relation of these variables with age and gender of the patients. In this study, patient with primary deep neck infections referred to ear, nose and throat ward during 1998 to 2009, were studied. After definite diagnosis of infection in head and neck spaces, the exact infection-causing agent and its complications were reviewed. Furthermore, age and sex of patients, co-existing symptoms, bacterial culture results causing the infection, disease background, presence or absence of addiction reviewed and information obtained in the questionnaire were recorded. For comparison Chi-square test was used. The most common cause of deep neck infection, was dental infection in 130 patients [(49%); 78, male; 52, female] followed by tonsillar infection in 44 patients (16.6%). The deep neck infection space types: simultaneous spaces includes sub-mandibular, sub-mental, sub-lingual (Ludwig's angina) in 59 patients (45.4%), then sub-mandibular space in 54 patients (41.5 %) and para-pharyngeal space in 32 patients (24.6%) were more common. Bacterial culture were done only in 50 patients, of which, 28 cases of bacterial growth occurred. The most common organism was streptococcus viridians in 18 patients. The deep neck infections are dangerous and life-threatening that often occurs due to dental infections. Therefore, it seems that promote public knowledge about oral health, to increase the need of dental and treatment centers, timely cure and manage the infections, which play an important role in reducing the deep neck infections.
  S. Razie , M. Panahi , A. Ahangarpor , F. Rahim and G. Saki
  This study was designed to evaluate the ultrastructure of contra lateral testis tissue and measurement of Serum inhibin B following unilateral blunt testis trauma. Twenty pre-pubertal male wistar albino rats aged 3 weeks were divided into 4 equal groups that each containing five rats. Group I was the control group. Group II was used as a Sham group. Group III had right orchiectomy initially. Group IV was the trauma group in which the right testis was placed on a firm sterile surface and the metal rod weighting 100 g was drooped on to the testis from a height of 5.5 cm. Seven weeks after initial operation 3 mL blood samples were obtained from each rat to determine inhibin B levels and contra lateral orchiectomies were performed in all groups to microscopically investigate electron. Inhibin B levels decreased in groups 3 and 4. The difference between group 3 with groups 1 and 2 was significant (p = 0.003 and 0.02). Also the difference between group 4 with groups 1 and 2 was significant (p = 0.006 and 0.002) but the difference between group 3 and 4 was not significant (p = 0.08). In group III (orchiectomy) TEM showed a normal sperm morphology and normal disruption of different stages of the spermatogonial maturation. Debris and vacuolar changes were seen in sertoli cells. Morphology of leydig cells slightly modified and the dilated cisternae of the Smooth Endoplasmic Reticulum (SER) were observed in group IV( trauma) mitochondria with degenerated cristae and enlarged vacuole were observed.
  G. Saki , S. Razie and S. Amirpoor
  The present experimental study was designed to investigate to elucidative effect of forced swimming stress on female mice fertility with emphasis on oocyte number and their quality and ultimately their in vitro fertilization capacity. A total 30 adult NMRI (Noda Medical Research Institute) mice randomly divided into two equal groups (n = 15); 1) control and 2) experimental groups. The control group remained in their cages, but experimental group was submitted to forced swimming for 3 min in water at 32°C daily for 50 days. The NMRI female mice in two group of study who mention above were administered intra-peritoneally with 5 IU pregnant mare serum gonadotropin (PMG) for superovulation. This was followed 46-48 h later by the intraperitoneal administration of 5 IU Human Chorionic Gonadotropin (hCG). Mice were euthanized 12-14 h after HCG injection by cervical dislocation method and oocytes collected from fallopian tube. Then count and quality of oocyte were assessed. In order to evaluated of fertilization capacity, a pre-incubated capacitated sperm was gently added to the freshly collected ova of two groups of study and two-cell embryos was counted 24-26 h after completion of fertilization in vitro. The fertilization capacity of oocyte of exposed to forced swimming stress was significantly lower than control groups (p<0.05). The plasma level of FSH and LH was significantly increased in stress mice group (p<0.05). The data of this present study clearly showed that forced swimming stress do not alter number of oocyte extraction but quality of extracted oocyte significantly decrease in mice exposed to forced swimming stress.
  F. Rahim , G. Saki and M. Bazrafkan
  This study is an attempt to elucidate the effect of alcoholic extract of the Ruta graveolens L. on the sperm count, motility and in vitro fertilization capacity of Wistar rats. Total 24 adult male Wister rats, 90- day-old and weighing 210±10.6 g were used in this study. All animals were housed individually per cage under a 12 h light/dark cycle, 20±2°C temperature and 60-65% humidity-controlled room with food and water ad libitum. All counts were performed at 37°C in T6 media. The sperm motility was assessed and classified as progressive, no progressive. Initial sperm motility was manually assessed by a single individual in duplicate for each sample by evaluating 100 sperms. In every group of this study 8 adult male rats were used. The sperm count was 2798.5±192.40 in group 1, 2801.8±418.67 in group 2 and 1017.4±820.69 in group 3. Therefore, group 3 has a significant lower sperm count in comparison with other groups. Progressive sperm motility was 57.20±2.81 in group 1, 55.25±1.82 in group 2 and 19.26±3.17 in group 3. The analysis shows that rats in group 3 have significant lower sperm motility in comparison with other groups. The fertilization capacity of sperm of rats in group 3 was significantly lower than other groups. As a conclusion, the alcohol extract of Ruta graveolens L. can be suggested as agent against male fertility but the exact mechanism of action is not understood yet so more experimental shall be done to reveal its effect as a contraceptive plant.
  F. Rahim , G. Saki , B. Ghavamizadeh , A. Jafaee and M. Kadkhodaee
  This study was conducted in vitro to show the effect of oxamate on motility and fertility of the mouse. The spermatozoa were extracted from the caudal part of epididymis. The study animals divided into four groups: (1) control group in TYH medium+5 mg mL-1 BSA ; (2) test groups in TYH medium in which contain 10 Mm oxamate; (3) test groups in TYH medium in which contain 20 Mm oxamate and (4) test groups in TYH medium in which contain 30 Mm oxamate. All four groups were incubated for 90 min to obtain capacitation. Further their motility was checked after incubation time. The mice were super ovulated with PMSG and HCG hormones to obtain oocytes. Total of 600 oocytes were collected and cultured in drops of KSOM medium+5 mg mL-1 BSA, then for fertilization process received spermatozoa from different groups of mentioned above. After 24-26 h, the rate of fertilization was checked. The results of this research indicated that oxamate at the concentration of 20 and 30 Mm significantly reduce (p<0.05) the progress of motility and fertility. Statistical analysis showed that percentage of the sperm progress motility in both concentration of 20 and 30 Mm was significantly differ (p<0.05) in compare with control group and in concentration of 10 Mm, respectively. The same results were obtained in the case of fertility. These findings suggested that oxamate has an inhibitory role on motility and fertility of mouse sperm.
  M. Bazrafkan , M. Panahi , G. Saki , A. Ahangarpour and N. Zaeimzadeh
  In this study the effect of aqueous extract of RG on spermatogenesis of rat has been investigated. Animals were allocated into three groups as: (1) control which did not receive anything, (2) vehicle which received only normal saline (the same volume as 3rd group according to the weight) and (3) experiment which received ruta extract (300 mg kg-1 intraperitoneally once a day for 50 days). A day after last injection the animals were deeply anesthetized and dissected. The right testes were extruded and fixed for histological studies. For statistical analysis ANOVA and Tukey as a post hoc. test were used. There was a significant decrease in the number of spermatogonia (p<0.01), primary spermatocyte (p<0.05), spermatid (p<0.05) and lydig cells (p<0.01) in experimental group as compared to control and vehicle. Also there were a significant increase in thickness of tunica albugina (p<0.01) and decrease in seminiferous tubule diameter (p<0.05) in experimental group compared to control and vehicle. So, it is concluded that the aqueous extract of Ruta graveolens diminishes the reproductive system activity and might be a useful substance for birth control process.
  M. Jasemi , G. Saki and F. Rahim
  To evaluate the effect of varicocele repair on sperm motility, sperm condensation and pregnancy rate in varicocele patients who referred to Department of Urology of Golestan and Apadana hospitals. The records were retrospectively evaluated for infertile patients with palpable varicocele who underwent varicocelectomy at Department of Urology of Golestan and Apadana Hospitals, Ahwaz, Iran from December 1990 to September 2008. The semen analysis of patient at pre-operatives and 3, 6 and 12 months post-operative and also pregnancy rate was calculated at 3, 6 and 12 months of follow-up. Before operation, no significant differences were seen among patients with right and left varicocele in sperm motility and condensation (p>0.05). Significant differences were seen in sperm motility and condensation of patients with bilateral varicocele compared to another patients (p<0.05). After surgery in three groups of patients with varicocele, the sperm motility and condensation significantly increased (p<0.05). Percentage of spontaneous pregnancy in 1 year after varicocele treatment is 31 to 40% of cases. The results of present study have shown that repair of varicocele by surgery significantly improved sperm motility, condensation and also 31-40% of infertile patients achieved pregnancy spontaneously.
  F. Rahim , A. Ebadi , G. Saki and A. Remazani
  This research was performed to study the prevalence of Congenital Heart Diseases (CHDs) in Iranian population. Data on the prevalence of CHDs were collected and analyzed from the major hospitals of Ahwaz, Khuzestan Province, Iran from the year 1998 to 2007. The present investigation in Ahwaz, from 1998 to 2007, revealed a mean prevalence of 12.30 per 1000 live births of the total 3061 inpatients and live births with a yearly prevalence varying from 7.93 to 17.51 per 1000 live births. The most frequent type of CHD was found to be Atrial Septal Defect (ASD) (19.54%) followed by Tetralogy of Fallot (TOF) (16.99%), Patent Ductus Arteriosus (PDA) (17.97%), Shunt (11.47%) and Ventricular Septal Defect (VSD) (11.07%). The prevalence of CHDs in Ahwaz is increasing from 1998 to 2007 which might be due to the improvement of diagnosis, attention or awareness among the medical authorities on the disease. The prevalence of CHDs in Ahwaz is not very high, however; it is an important disease which needs an immediate medical attention.
  N. Saki , F. Rahim , S. Nickakhlagh , H. Dehdashti and G. Saki
  This retrospective study was conducted to define the relationship of between existences of the bronchial cysts and fistulas with gender of patients as well as review of literature. Data were collected from the records of total 149 patient files (63 male and 86 female) with including history of having branchial cleft anomalies (cysts and fistulas), that admitted in ENT Department of Imam Khomeini and Apadana Private Hospitals between 1998 and 2008. The analyzed characteristics include gender of patient, anatomical location and type the anomaly. The most frequent type of branchial cleft anomalies in cyst and fistula disorders was second branchial with the values of 85.71% in 21 male patients, 37.5% in total 48 patients; 76.19% in 42 male patients, 31.68% in total 101 patients and values of 62.97% in 27 patients, 35.41% in total 48 cases; 76.27% in 59 female patients, 44.55% in total 101 cases, respectively. There was no significant difference in frequency (p>0.05) between male and female patients as well as the locations of anomalies in neck. The overall frequency of the second branchial cleft in different types of disorders such as cysts and fistulas in Southwest region of Iran is the highest, which was similar to international findings. In summary, anatomical location and type of congenital neck masses help narrow the differential diagnosis.
  S. Movassaghi , G. Saki , F. Javadnia , M. Panahi , M. Mahmoudi and F. Rhim
  MBCD and Cholesterol-Loaded-Cyclodextrin (CLC) were examined for their abilities to increase the cryosurvival of C57BL/6 mouse sperm, the main strain of genetically engineered mice. The intactness of acrosome and motility of frozen/thawed spermatozoa were used to monitor cryosurvival. In this experimental study, male mice were randomly divided in 6 groups: control 1, experimental 1, experimental 2, control 2, experimental 3 and experimental 4. In experimental groups 1 and 2 spermatozoa were exposed to 0.75 and 1 mM MBCD and in experimental groups 3 and 4 were exposed to two different concentrations of CLC (1 and 2 mg mL-1) over a period of 1 h and were subsequently cryopreserved. Spermatozoa in control 1 group were frozen without any exposure to CLC or MBCD and in control 2 (vehicle), sperms were incubated with 4 mM MBCD. The post-thaw sperms were evaluated for their motility and acrosomal status. The values of the intact acrosome and motility increased significantly with concentration of CLC compared to controls and MBCD experimental groups (p<0.05). These results indicate that cryosurvival of C57BL/6 mouse spermatozoa is enhanced by exposure to MBCD which loaded with cholesterol (CLC) before freezing and MBCD alone can not protect sperm from freeze-thaw damage efficiently compare to CLC.
  M. Davoodi , N. Saki , G. Saki and F. Rahim
  The aim of this research was to study of the relationship between anatomical variations of neurovascular structures adjacent sphenoid sinus with sex and position of appearance by using CT scan. In this retrospective study paranasal sinuses CT scan has been taken from 399 patients (210 male, 189 female) that referred to Imam Khomeini and Apadana Hospitals, Ahwaz, Iran. Furthermore, protrusion and dehiscence of Internal Carotid Artery (ICA), Maxillary Nerve (MN), Vidian Nerve (VN) and Optic Nerve (ON) into the sphenoid sinuses cavity have been investigated by using CT scan results. In 210 male patients the protrusion of interested variables were noticed as: ICA in 102 (48.5%) cases, ON in 80(38%) cases, MN in 74 (35.5%) cases, and VN in 60 (28.5%) cases, respectively. Also in 189 female patients group the protrusion of ICA, ON, MN, VN were noticed in 65 (34.3%), 66 (34.9%), 62 (32.8%) and 43 (22.7%) cases, respectively. The statistical analysis show significant difference (p = 0.001) of protrusion of ICA between male and female groups. In 210 male patients the dehiscence of ICA, ON, MN, VN were noticed in 82 (39%), 60 (28.5%), 60 (28.5%) and 66 (31.4%) cases, respectively. Also in 189 female patients the dehiscence of interested variables were noticed as: ICA in 85 (44.9 %), ON in 87 (46%), MN in 69 (36.5%), VN in 71 (37.5%) cases, respectively. The statistical analysis show significant difference (p = 0.03) of dehiscence of on variable in male and female groups. In order to increase the risk of intra-operative complications detailed preoperative investigation of neurovascular structures in sphenoid sinuses by use of CT scan images should be done properly.
  N. Saki , F. Rahim , S. Nikaghlagh and G. Saki
  Foreign body in the esophagus is a common emergency presentation. Foreign body aspirations comprise the majority of accidental deaths in childhood. Conventional x-ray imaging is usually obtained to aid the diagnosis during the initial evaluation. The decision for surgical intervention is usually based on a suspicious history, physical examination and radiologic findings. Rigid bronchoscopy is very effective procedure for inhaled foreign body removal with fewer complications. Proper use of diagnostic techniques provides a high degree of success and the treatment modality to be used depending on the type of the foreign body is mostly satisfactory. Live foreign body is a rare entity but common emergency presentation. The approach towards a patient with leech infestation comprises a thorough history and systematic examination followed by relevant investigations. However, there is considerable debate over the most appropriate treatment option for such patients. A living foreign body, or parasite, in the oro-or naso-pharynx is rare in Western countries, but in other parts of the world is a fairly common cause of problems. In the past 10 years 28 cases of foreign bodies due to infestation with leeches were treated in our departments. In all patients, 2-70 years old, the complaint was of recurrent episodes of epistaxis, blood-spitting, odynophagia, dysphagia, dyspnea and hemoptysis several days before admission. Examination showed a green-brown mass protruding from different naso- and oro-pharengeal areas, which is in every case, was a blood-engorged leech. Treatment consisted of removing the leech by applying a forceps to the middle of the leech's body and giving a quick pull. Bleeding ceased immediately after removal of the leech. This review aims to develop a comprehensive approach towards patients presenting with foreign body ingestion by developing clinical practice guidelines. These guidelines address not only the initial evaluation of the patient but also the various management alternatives and their advantages, limitations and applicability in various scenarios, based upon a review of the literature.
  A. Sarkaki , H. Fathimoghaddam , S.M.T. Mansouri , M. Shahrani Korrani , G. Saki and Y. Farbood
  Cerebral Hypoperfusion Ischemia (CHI) has important role in neuronal damage and behavioral deficits, including memory and Long-term Potentiation (LTP) impairment. Protective effects of Gallic Acid (GA) on memory, hippocampus LTP and cell viability were examined in permanent bilateral common carotid artery occlusion in rats. Animals were divided into 9 groups: Control (Cont); sham operated (Sho); Cerebral Hypoperfusion Ischemia (CHI); CHI received normal saline (CHI +Veh); CHI treated with different doses gallic acid (50, 100, 200 mg kg-1 for 5 days before and 5 days after CHI induction, orally); CHI treated with phenytoin (50 mg kg-1, ip) (CHI+Phe); and sham operated received 100 mg kg-1, orally (Sho+GA100). CHI was induced by bilateral common carotid artery occlusion (2VO). Behavioral, electrophysiological and histological evaluations were performed. Data were analyzed by one-way and repeated measures ANOVA followed by tukey’s post-hoc test. GA improved passive avoidance memory, hippocampal LTP and cell viability in hippocampus and cortex of ischemic rats significantly (p<0.01). The results suggest that gallic acid via its antioxidative and free radicals scavenging properties attenuates CHI induced behavioral and electrophysiological deficits and has significant protective effect on brain cell viability. Dose of 100 mg kg-1 GA has affected the ischemic but not intact rats and its effect was more potent significantly than phenytoin, a routine drug for ischemic subjects.
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