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Articles by G. Rahimi
Total Records ( 8 ) for G. Rahimi
  M. Sadeghi , M. Moradi Shahr-e-Babak , G. Rahimi and A. Nejati Javaremi
  The objective of this study was to obtain genetic marker information in the Iranian Holstein bulls by analyzing bGH gene polymorphism and to verify its association with milk production traits. One sample including 134 Iranian Holstein bulls was genotyped at bGH gene restriction sites (bGH-AluI). Gene frequencies of L and V alleles were 0.936 and 0.064, respectively. Genotypes are distributed according to the Hardy-Wienberg equilibrium. Differences in the milk and fat yield between the two genotypes approached significance (p≤0.10). Bulls with LL genotype had higher milk, fat yield compared to LV genotypes. Present study showed that the L allele was associated with protein yield (p≤0.021). No associations (p>0.1) were found between the genotypes and fat and protein percent. The dominant L allele at the bGH locus favours higher production of milk protein. It can be concluded that this locus is a QTL or is in disequilibrium linkage with one or more tightly linked QTL. Thus it could be a useful DNA marker for milk production traits in dairy cattle breeding.
  M. Sadeghi , M. Moradi Shahr-e-Babak , G. Rahimi and A. Nejati Javaremi
  G. Rahimi and M. Hassanzadeh
  The present study was carried out to investigate the influence of energy and protein contents of the diet on growth performance and to evaluate further interactions of dietary manipulation and genotype on endocrine parameters in broiler chickens. 1200 day-old male and female broiler chicks from 2 commercial broiler strains (Hybro and Ross) were fed ad libitum from day 1 onwards by 4 different diets either with a high or low energy combined with a high or low crude protein content. Body weight and feed intake were measured weekly. Venous blood samples were taken (10 birds/sex /line/diet group) weekly from day 14 on, for plasma Growth Hormone (GH), Triiodotyronine (T3) and Thyroxin (T4) content. Relative growth rate was higher in broiler chickens which fed the low energy diet compared to high energy diet during the first week of age, while from week 1 on, a reverse effect of energy on relative growth rate was found. The effect of energy and protein on feed intake was significant (p<0.05), while no interaction between energy and protein was found. Within high energy diet, birds fed low protein diet showed a tendency for higher T3 levels, while on low energy diet, broiler chicks fed high protein level showed a tendency for higher plasma T3 concentrations, probably explaining the presence of a significant energyĂ—protein interaction. Protein content of the diet, significantly (p<0.05) affected plasma T4 levels while energy content had no effect. The higher GH as well as T3 levels in the younger chicks may be an indicator of higher requirement of these hormones for protein synthesis during the fast growth phase. It can be concluded that higher protein content of the feed could result in lower T3, higher T4 and higher plasma GH levels. Possible causal mechanisms underlying this phenomenon are discussed.
  A. Khan Ahmadi , G. Rahimi , A. Vafaei and H. Sayyazadeh
  In the present study the genetic structure of Pekin and Moscovy duck populations in north of Iran, Mazandaran province were analyzed using thirteen microsatellite markers. One hundred blood samples with equal number from each population were collected and DNA was extracted using modified salting out method. After Polymerase Chain Reaction (PCR), the PCR products were electrophoresed using 6% polyacrylamide gel. Four Out of 12 microsatellite markers were not amplified in any of both populations, three markers were monomorph and six markers generated polymorph bands. Some genetic parameters such as observed and effective allele number, mean of heterozygosity and genetic distance between two duck populations were estimated. The observed allele number in each locus was ranged from 1-4, effective allele number from 1-3.78, heterozygosity from 0-0.98 and the genetic distance between two populations was measured as 0.59 percentages. The low of genetic distance between two populations and the low level of mean heterozygosity index indicate that the genetic diversity is low in within and between populations. The low mean heterozygosity may be attributed to the low number of alleles present in the population, high level of inbreeding values because of small effective population size involved in studied duck breeding flocks. The analysis of Hardy-Weinberg equilibrium test showed that none of the microsattelite sites were at equilibrium. The obtained results at the present study indicated that characterization of genetic diversity by employing molecular tools is a prerequisite in developing strategies for conservation and utilization of duck genetic resources.
  S. Zakizadeh , M. Reissmann , G. Rahimi , A. Nejati Javaremi , P. Reinecke , S.R. Mirae-Ashtiani and M. Moradi Shahrbabak
  The aim of this study was to estimate the allele frequencies in polymorphic site of exon six of POU1F1 gene in three Iranian native and Holstein cattle. Genomic DNA was extracted from 3 Iranian native cattle breeds, including 97 Mazandarani, 87 Sarabi, 112 Golpaygani and also 110 Holstein cattle. A 451 bp fragment of intron 5 and exon 6 were amplified and digested with HinfI restriction enzyme. Frequencies of allele A were 0.37, 0.27, 0.34 and 0.21 for Mazandarani, Sarabi, Golpaygani and Holstein cattle, respectively. Significant differences in genotype frequencies were found between Mazandarani or Golpaygani and Holstein cattle. No significant differences in genotype frequencies were found between Sarabi and Holstein cattle. Transition A to G in nucleotide 1256 is responsible for HinfI(-) allele. No significant association was observed between POU1F1 polymorphism and milk production. Differences in allelic frequency between native Bos indicus breeds (Mazandarani, Golpaygani) and Holstein at the present study might be due to differences in origin breeds, low number of samples and/or as the effect of natural selection in native breeds.
  S. Zakizadeh , A. Nejati-Javaremi , M. Reissmann , G. Rahimi and A. Jahan Bakhshi
  PCR-RFLP was used to genotype 87 Sarabi native cattle of north-western Iran for A and B alleles of kappa casein gene. A 350 bp length of exon 4 and intron 4 was amplified and digested with HinfI endonuclease. Samples were loaded on agarose gel (2%) and genotyped under UV light. Allele frequency of desirable B allele was 0.57. Stochastic simulation was used to generate milk yield trait for a population of 4950 females and 50 males for 15 overlapping generations. Population parameters included 1100 and 436 kg for average milk yield and phenotypic deviation, respectively; with heritability of 0.27. Additive and dominance effects of Kappa Casein gene were considered as 187.63 and 50.37 kg, respectively. Two methods were considered for selection of males based on the first phenotypic record of their dams (PAS) or molecular information of each male, individually (GAS). Females were always selected on their first phenotypic record. Although, there was a significant difference between polygenic and major gene genetic response between two methods after the 5th generations, but there was no significant difference for the sum of polygenic and major gene response. After 15 generations of selection there was no significant difference between inbreeding coefficient under two methods. Selection plan for males based on one single major gene had no advantage over the conventional selection based on dam record in native Sarabi breed.
  J. Fayazi , M. Moradi , G. Rahimi , R. Ashtyani and H. Galledari
  This study was conducted to evaluate the genetic structure of B. xanthopterus populations in Karoon, Krkhe and Jrahi rivers in southwest of Iran using Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) analysis of mitochondrial DNA (mtDNA). Amplified mtDNA (cytochrome b and part of tRNAGlu) genes were digested with nine restriction enzymes in order to check the presence of recognition sites. RFLP analysis revealed a total of nine composite haplotypes in 100 individuals. The estimated sequence divergence between all pairs of haplotypes ranged from 0.8 to 4.4%. The haplotype and nucleotide diversity within populations ranged from 0.3785 to 0.6456 and 0.0052 to 0.0125, respectively. The average nucleotide diversity (πxy) and the net nucleotide divergence (δ) among 3 populations ranged from 0.0108 to 0.0127 and 0.00 to 0.39%, respectively. The neighbour-joining dendrogram which was constructed based on the net nucleotide sequence divergence showed that B. xanthopterus populations separated in two groups. The B. xanthopterus populations in Karoon and Krkhe rivers are categorized in one group and Jrahi river in a second group. The obtained phylogenetic relationships was approved by Fitch-Margoliash method using Slatkin linearized FSTs. The obtained results at the present study showed a low genetic diversity between B. xanthopterus of Karoon, Krkhe and Jrahi rivers in southwest of Iran. The results also suggested that for maintaining of genetic diversity of B. xanthopterus at appropriate levels, the exchange and transfer of Barbus stocking between Jrahi and other river basin should be avoided.
  G. Rahimi , A. Nejati-Javaremi and K. Olek
  The objective of the present study was to estimate the allele and genotype frequencies of the BLAD, DUMPS and κ-CSN genes in Holstein young bulls of the national AI station of Iran. It is shown that the genetic mutation responsible for BLAD in Holstein cattle was absent in all young bulls entering to the national dairy cattle progeny test. Of the 37 young bulls genotyped for CD18/HaeIII gene mutation, 36 were normal homozygote (AA), 1 was heterozygote for the disease (AB) and no affected homozygote genotype (BB) were found. The frequency of the normal and BLAD mutant allele were 0.99 and 0.01, respectively. The analyzed results showed that the BLAD carrier prevalence in Iranian Holstein young bulls was approximately 2.8%. The genotype frequencies in κ-CSN/HinfI locus were 0.70 for homozygote (AA) and 0.30 for heterozygote (AB) animals while the frequency of the A allele was 0.85. In the present study it is shown that the genetic mutation responsible for DUMPS was absent in all of the young bulls analyzed from national AI station. It can be concluded that due to negative economical impact of the DUMPS and BLAD mutation on dairy cattle industry and their recessive mode of inheritance, attention has to be paid to any case of a young bulls entering to the testing program having in his origin any known carriers. Such a bull should be tested and if positive eliminated from the active population. Young bulls (under progeny testing program) should also be screened for both mutated alleles if in their progeny a high incidence of mortality is observed. This strategy would prevent the introduction of these mutated alleles in the Iranian endogenous cattle breeds.
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