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Articles by G. Lokesh
Total Records ( 3 ) for G. Lokesh
  G. Lokesh , Putkho Paul Pao , K.N. Madhusudhan , P.K. Kar , A.K. Srivastava , M.K. Sinha , R. Manohar Reddy , P.M. Muniswamy Reddy and B.C. Prasad
  The wild silkworm, tropical tasar Antheraea mylitta Drury being mainly culturing in central and eastern parts of India and produces famous tasar silk. This insect species expresses divergent phenotypic characters in response to varying ecological and climatic conditions thus exists as ecoraces. Hence, in the present study, three ecoraces viz., Daba, Sukinda (Semi-domesticated) and Sarihan (wild) have been selected for the assessment of the variability in silk gland related traits. The parameters of the silk glands studied like comparative length, weight and silk gland to larval body mass index. Besides, some biochemical contents of the silk gland tissue were also studied in three ecoraces. The results showed significant variations at p<5% in all the parameters among the three ecoraces studied, also showed the sexual dimorphism in expression of silk gland features and economic traits. The semi-domesticated Daba ecorace showed higher performance in most of the parameters with comparatively greater in silk gland weight (22%) and in body mass index (>16%). Apart from this, wild ecorace Sarihan cocoons showed higher shell ratio (>10% over the value of other ecoraces). The study infers that, there is wide variability in the expression of phenotype with respect to silk gland related traits among the different ecoraces of tropical tasar silkworm A. mylitta. Since the silk glands are site of biosynthesis of silk proteins, these may be considered for the assessment of diversity or characterization of different ecoraces of tropical tasar silkworm.
  G. Lokesh , S.R. Ananthanarayana and V.N. Yogananda Murthy
  The silkworm Bombyx mori L. is a economic sericigenous insect show a high degree of sensitivity towards chemical mutagens and have drastic physiological and biochemical effects during its larval stages. In the present study a bivoltine race of silkworm NB4D2 was treated with chemical Mutagen Diethyl Sulfate (DES). The larvae were used for the treatment with different concentrations of DES orally as well as injected through body wall. In F1 generation, the larvae of 4th and 5th instar were used to study the activity of prominent digestive enzymes amylase and protease for evaluation of the mutagenic response in haemolymph and midgut tissues. The enzyme activity was found to be higher in treated sets, particularly in 8 mM injection sets. Further, maximum enzyme activity was noticed during middle part of both the instars with a few variations among the treated sets. The data were statistically analysed using One-way ANOVA and discussed.
  G. Lokesh , M. Maheswari , Ritwika Sur Chaudhuri , D.S. Somaprakash , S. Sekar and R.K. Mishra
  Background and Objective: Genetic diversity and variability in the population are pre-requisite for the crop improvement programme. Collection and maintenance of genetic diversity is a fundamental component in long-term management strategies for genetic improvement of silkworm. The silkworms mutant were evaluated for morphological characters and rearing traits during two crop seasons i.e., winter and summer to analyze the performance and to identify better mutant accessions. Materials and Methods: Total 23 mutant silkworm accessions were considered for the study, the important morphological characters during egg, larva and cocoon stages were recorded and compared with the catalogue data. Total 12 important silkworm quantitative traits were studied and analysed using multi trait analysis package to identify better accessions. Results: Significant amount of variations were observed among different accessions for different traits. Among 23 mutant silkworms 08 were qualified in the cumulative Evaluation Index (EI) (>50). The cluster analysis showed heterogeneity among the silkworm accessions based on the grouping. The Principal Component Analysis (PCA) indicated the grouping of 06 mutants along with commercially important silkworm races Multivoltine Pure Mysore (PM) and bivoltine (CSR-2). Conclusion: Since these genotypes were considered most suitable for basic genetic studies rather its usefulness in silk production. The correlation studies using PCA revealed that some of the mutant silkworms conserved in the germplasm showed on par with commercially important silkworm races and can be used to explore the combining ability studies for further commercial exploitation.
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