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Articles by G. Krishnappa
Total Records ( 5 ) for G. Krishnappa
  S.J. Arun and G. Krishnappa
  Outer Membrane Protein (OMP) - enriched extracts of Pasteurella multocida A:1 (`A`) and local isolate (`L`) obtained from Nutrient rich free cell (FC), Nutrient deficient free cell (DC) and Biofilm cell (BC) growth were analyzed by SDS-PAGE. In general, the OMP profile in all the three growth conditions of `A` was different from that of `L` suggesting that both could be of different serotype. The FC, DC and BC derived OMPs of both `A` and `L` showed a significant variation suggesting that BC are demonstrably and profoundly different from their free cell counterparts (FC and DC). More than four unique proteins were observed in the OMPs of BC when compared to FC and DC. The western blot using Anti-BC hyperimmune serum, elicited maximum number of immunogens that were common to both `A` and `L`, including immunogens that were detected by Anti-DC and Anti-FC. Some unique proteins of biofilm cells were also found to be highly immunogenic. Since, fowl cholera is a biofilm associated disease the use of biofilm form of pathogen could be an alternative strategy for evolving effective immunoprophylaxis against fowl cholera.
  B. Prakash , T. Suryanarayana , L. Muniyappa and G. Krishnappa
  Indirect enzyme linked immunosorbent assay was developed to measure specific antibody activity to outer membrane proteins in sera and egg yolk of chickens exposed to Salmonella Gallinarum infection and also following vaccination. The observed end point titers were used to formulate regression equation and the absorbance data obtained at a single serum dilution were converted directly to antibody titer by subtraction method. The assay was carried out at a single serum dilution of 1:320 standardized and the antibody titers of the Salmonella Gallinarum infected flocks were found to be of higher magnitude when compared to vaccinated flocks. The growth-decline trend of the egg yolk antibody levels was found to be similar to that of the serum antibody levels.
  Amitha R. Gomes , L. Muniyappa , G. Krishnappa , V.V.S. Suryanarayana , S. Isloor , B. Prakash and P.G. Hugar
  Fifteen serotypes of E. coli isolated from poultry were subjected to RAPD-PCR using eight oligonucleotide random primers. Amplification with each of the primers resulted in generation of different DNA fingerprinting profile with varied number of bands. Dendrogram based generation of clustering of E. coli serotypes showed two major clusters. The polymorphic amplicons served as markers for E. coli species where as the unique amplicons served as markers for E. coli serotypes. RAPD based fingerprinting provided a rapid means of discriminating E. coli serotypes and considered a valuable tool for molecular typing.
  B. Prakash , G. Krishnappa , L. Muniyappa and B. Santhosh Kumar
  The study was undertaken to elucidate the recent epidemiological status of salmonellosis in India. A total of 23 Salmonella isolates were recovered from different disease outbreaks in different geographical locations. The phenotypic analysis by serotyping, transmission electron microscopy and antibiogram profiles were able to classify the Salmonella isolates based on different serovars. The S. Gallinarum isolates were also classified into different strains based on the resistance pattern to different antimicrobials used. The plasmid profiles of the Salmonella isolates were also found to be serovar specific and the S. Gallinarum isolates were subdivided into different strains based on the geographical origin. There was a positive correlation between the antibiotic resistance pattern and the presence of plasmid within different serovars.
  B.M. Chandra Naik , S.R. Santhosh , B.Y. Sridhar , Asha Mayanna , Amitha R. Gomes , B.R. Harish , G.S. Mamatha , S.R. Jayakumar and G. Krishnappa
  Trachea and lung tissue samples collected from a disease outbreak at Regional Poultry Farm, Kerala, India were submitted for virological investigation at Institute of Animal Health and Veterinary Biologicals (IAH & VB), Bangalore. The outbreak had taken a toll of 385 layer parent stock birds. The main symptoms and lesions were air saculitis, tracheal rales, oopheritis and salphingitis. The samples were processed and inoculated to 10th day old chicken embryos. Lesions of dwarfing and curling characteristic of Infectious bronchitis were noticed in inoculated embryos. The virus was confirmed by a modified Haemagglutination (HA) test, wherein the allontoic fluid was pretreated with crude filtrate of Clostridium perfringens before carrying out HA.
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