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| Articles
by
G. Balasubramanian |
Total Records (
3 ) for
G. Balasubramanian |
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P.C. Prabu
,
C. Udayasoorian
and
G. Balasubramanian
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A new lignin degrading basidiomycete, Phanerochaete chrysosporium (TL 1) was isolated from pulp and paper mill effluent enriched soil samples can be induced to produce high level laccases when grown on a cellobiose-asparagine liquid medium with 150 μM CuSO4. The fungus grown under static conditions had 70% of total extra cellular laccase protein and about 2.5 fold purification with a final yield of 13.2% of protein purification by Sephadex G-100 column and FPLC. The resultant enzyme pool of the purification process is found to contain a single polypeptide, which produced a single band on an SDS-PAGE. The purified protein showed a specific activity of 106 U mg-1 and the molecular mass (Mr) of native laccase was 65 kDa. The purified laccase has an isoelctric point of 4.0, it is stable in pH range from 4.0 to 6.0 and its optimum pH is 4.5. The optimal reaction temperature is 60°C and stable at 70°C for more than 1 h. Degenerative primers corresponding to the consensus sequences of the copper binding regions in the N-terminal domains of known basidiomycete laccase were used to isolate laccase gene specific sequences from this strain and the laccase gene gave PCR product of about 150 bp and cloned product gave 85% similarity with laccase from T. villosa LCC 2 (L49377). |
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C. Udayasoorian
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P.C. Prabu
and
G. Balasubramanian
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The white rot fungus isolated from continuous pulp and paper mill effluent irrigated soil and identified as Phanerochaete chrysosporium (TL 1) was capable of degrading pentachlorophenol. 14C synthetic lignin mineralization assays showed that the fungus assimilated 33.6% of the total label. Removal and degradation of pentachlorophenol (PCP) by the organism in static flask cultures was studied using ammonium lignosulphonate (LS), a waste product of paper mill industry, as a carbon and nitrogen source. After eight days, Phanerochaete chrysosporium (TL 1) grown in 2% LS (nitrogen sufficient) medium removed 85% of PCP which was comparable that of degradation in 2% glucose medium (93%). The presence of laccase (EC.1.10.3.2) extracellular activity suggested that the fungus secrete the enzyme into the extracellular medium, which was responsible for the degradation on PCP. The extracellular laccase activity was assayed on agarose plates containing ABTS. |
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M. Sainabha
,
N. Muthukumar
,
K. Ramkumar
and
G. Balasubramanian
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The role of advanced control techniques for an energy intensive
chemical process like paper industries is indispensable. The quality of the
end product depends entirely on the efficiency and disturbance managing capacity
of the control techniques. In this background the role of Model predictive control
as a constraint handling as well as quality assuring tool has to be studied.
In this study, two different model predictive controller techniques namely Dynamic
Matrix Control (DMC) and Generalized Predictive Controller (GPC) is considered.
The controllers are suitably designed for controlling the moisture level for
a bench marked paper machine model. The servo performance of the DMC and GPC
controllers under unconstrained environment is studied for and the results are
tabulated in this study. The study reveals that the Dynamic matrix controller
(DMC) provides better performance than the Generalized Predictive Controller
(GPC). |
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