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Articles by Fuying Zheng
Total Records ( 4 ) for Fuying Zheng
  Fuying Zheng , Guozhen Lin , Changqing Qiu , Jizhang Zhou , Xiaoan Cao and Xiaowei Gong
  The infected blood was collected from dairy cattle showing similar Bovine Ephemera Fever (BEF) clinical signs in Taizhou area of Zhejiang province. The blood frozen-thawed 3 times was concentrated decuple by centrifugation, then the virus had 7 blind passages in suckling mice by an intracerebral inoculation route and 10 passages in BHK21 cells. The suckling mice died regularly 3-4 days after inoculation from the 3rd passage with 100% mortality rate. In BHK21 cells, the CPE occurred from the secondary passage and 80% cells approximately emerged pathological changes post-infection from the 4th passage. The virus isolated was proved to be Bovine Ephemera Fever Virus (BEFV) by RT-PCR, Transmission Electron Microscopy (TEM), Virus Neutralization (VN) test and IFA test. The virus isolated was designated JT02L. The homologies of amino acid sequence of the G gene compared with JB76H (Beijing strain), Taiwan strain and BB7721 (Australia’s strain) all were above 90%.
  Fuying Zheng , Qiwei Chen , Xiaowei Gong and Yongsheng Liu
  Class 1-3 integrons from 87 strains of Escherichia coli, Salmonella, Riemerella anatipestifer and staphylococci isolated from pigs and ducks were screened and the variable regions of the class 1 and 2 integrons were amplified. No class 3 integrase gene fragments were amplified from any of the 87 strains; class 2 integrase gene fragments were amplified from only seven strains and no class 2 integron variable region gene cassettes were amplified. Class 1 integrase gene fragments were detected in 65 strains and variable region gene cassettes were amplified from 26 strains which were confirmed by sequencing to be the aadA2, dfrA12-orfF-aadA2, dfrA17-aadA5, aacA4-catB8-aadA1 and dfrA32-ereA-aadA2 gene cassettes. The 87 strains were tested for drug susceptibility to 10 kinds of antibiotics using the Broth Microdilution Method and minimum inhibitory concentrations. There was no significant difference in the resistance phenotypes of strains carrying gene cassettes and those not carrying gene cassettes and there was no correlation between the antibiotic resistance of a strain and whether it carried a resistance-gene cassette. The integron-mediated horizontal transfer of resistance genes might be just one resistance mechanism acquired by bacteria during their evolution and may not play a major role under the selection pressure exerted by the wide variety and high concentrations of antibiotics used at present, whereas it may play a synergistic role.
  Fuying Zheng , Qiwei Chen , Xiaowei Gong and Yongsheng Liu
  Class 1 integron is a genetic element with the ability to capture and express of the exogenous drug-resistance genes embedded in gene cassettes, offering bacteria with multi-drug resistance. The structures of the integron itself can regulate the transcription and express levels of the cassette genes. In this study, the effects of the attI sites on the transcription and express of the cassette and integrase genes were reported. pACYC184 plasmids, containing cloned integron fragments which differed only with respect to the lengths of attI sites were transferred into E. coli JM109. Then, the transcription and expression levels of the integrase and the first cassette gene after Pc promoter were detected in the corresponding recombinant strains. The results showed that the lengths of attI sites had no effect on the transcription and expression of the gene cassettes and integrase genes. This research was reported for the first time.
  Jizhang Zhou , Changqing Qiu , Guozhen Lin , Xiaoan Cao , Fuying Zheng , Xiaowei Gong and Guanghua Wang
  Twenty six hens which were suspected to suffer from chlamydiosis were detected with Indirect Hemagglutination test (IHA) and twelve sera samples of them were positive. The C. psittaci was isolated from yolk sac of embryonated eggs. The MOMP gene of C. psittaci was amplified with PCR and a strand of 1170 bp was got.
 
 
 
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