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Articles by Friedrich Herberg
Total Records ( 2 ) for Friedrich Herberg
  Jamsari , Lily Syukriani , Hashlin Pascananda Utami , Friedrich Herberg , Wolfgang Nellen and Istino Ferita
  Artificial inoculation is an important method for understanding the plant-virus interaction. Currently available techniques for virus artificial infection are whitefly based inoculation, grafting, mechanical, agroinoculation and particle bombardment. In fact, almost all the available inoculation methods do not result in ideal efficiency nor practicality. The purpose of this study is to establish an injection based technique for routine artificial inoculation of Geminivirus particle into chilli pepper plant. The following treatments were applied in this study: Different ratios of extract-buffer (0, 20 and 60% w/v) and injection at four different positions (no inoculation, shoot, middle of main vein and petiol). The presence of Geminivirus particles in plants whether for inoculum source and infection effectivity was confirmed using the polymerase chain reaction. Some morphological observations were also applied in measurement of infection effectivity. Overall, the incidence of infection was 18% and among these, the average intensity of disease was 53% in the four week post inoculation. Geminivirus transmission by injection technique described in this study could be a simple and effective method for geminivirus artificial infection. However, accuracy, simplicity and uniformity have to be accommodated for further application of this technique.
  Jamsari , Istino Ferita , Lily Syukriani , Helni Lalan , Friedrich Herberg and Wolfgang Nellen
  Whiteflies have attracted intensive attention from many agriculturists due to their direct feeding and plant virus transmission. The economic losses depend on certain species population existing on the field during chilli-pepper cultivation. More over, biotype characteristic will determine the effectivity of applied pest management. Based on this, detailed information on whiteflies in chilli-pepper cultivation is very important. We applied mitochondria cytochrome oxidase unit I gene sequence for detecting species and biotype of whiteflies existing in West Sumatera. Two species were successfully identified i.e., Bemisia tabaci and Trialeourides vaporariorum that are dominant in this region. Distribution of both species are separated by altitude, where B. tabaci exist predominantly from low altitude to medium altitude, whereas in high altitude it is not common. On the other hand, T. vaporariorum distribute mainly in high altitude and not in medium altitude. Furthermore biotype determination on B. tabaci identified the existence of B biotype in all areas studied.
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